Volume 33, Issue 10 (2-2026)
JSSU 2026, 33(10): 9509-9520 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rahmati Kukandeh M, Tangestanizadeh M, Arab H, Gharehkhani E, Shokrzadeh M. Investigating the Synergistic Effects of Aspartic Acid and Tocopherol on Cytotoxicity and Oxidative Stress Caused by Etoposide on Hepg2 Cell Line by MTT and ROS Methods. JSSU 2026; 33 (10) :9509-9520
URL: http://jssu.ssu.ac.ir/article-1-6382-en.html
URL: http://jssu.ssu.ac.ir/article-1-6382-en.html
Mahboube Rahmati Kukandeh 
, Maryam Tangestanizadeh , Hourolein Arab , Elahe Gharehkhani , Mohammad Shokrzadeh *
, Maryam Tangestanizadeh , Hourolein Arab , Elahe Gharehkhani , Mohammad Shokrzadeh *
Abstract: (4 Views)
Introduction: Liver cancer ranks among the deadliest cancers globally. Etoposide, a chemotherapeutic agent frequently utilized in treatment, inhibits topoisomerase II, leading to cell cycle arrest and apoptosis induction. Nonetheless, etoposide generates reactive oxygen species (ROS), resulting in oxidative stress and cellular damage. This study aimed to investigate the combined effects of two antioxidant compounds, alpha-tocopherol and aspartic acid, on cytotoxicity and oxidative stress by etoposide in the HepG2 cell line.
Methods: HepG2 cells were pre-exposed to various concentrations of alpha-tocopherol and aspartic acid (10, 25, 50, 100, 200, and 400 µM). Following pretreatment, cytotoxicity was induced using etoposide at its IC50 concentration (7.08 µM). Subsequently, lipid peroxidation, ROS concentrations, and cell viability parameters were measured.
Results: Based on cell viability measurements and oxidative stress tests, the combination of alpha-tocopherol and aspartic acid with etoposide enhanced cell survival across all tested concentrations. Additionally, they reduced MDA and ROS concentrations.
Conclusion: The findings of this study indicate that alpha-tocopherol and aspartic acid can mitigate the cytotoxic effects of etoposide and provide a protective role by enhancing the cellular antioxidant defense. These results highlight the potential use of this combination as a complementary therapeutic strategy in liver cancer treatment.
Methods: HepG2 cells were pre-exposed to various concentrations of alpha-tocopherol and aspartic acid (10, 25, 50, 100, 200, and 400 µM). Following pretreatment, cytotoxicity was induced using etoposide at its IC50 concentration (7.08 µM). Subsequently, lipid peroxidation, ROS concentrations, and cell viability parameters were measured.
Results: Based on cell viability measurements and oxidative stress tests, the combination of alpha-tocopherol and aspartic acid with etoposide enhanced cell survival across all tested concentrations. Additionally, they reduced MDA and ROS concentrations.
Conclusion: The findings of this study indicate that alpha-tocopherol and aspartic acid can mitigate the cytotoxic effects of etoposide and provide a protective role by enhancing the cellular antioxidant defense. These results highlight the potential use of this combination as a complementary therapeutic strategy in liver cancer treatment.
Type of Study: Original article |
Subject:
Biology
Received: 2025/02/7 | Accepted: 2025/05/6 | Published: 2026/02/4
Received: 2025/02/7 | Accepted: 2025/05/6 | Published: 2026/02/4
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
