TGF-β1 Gene Polymorphism at Position -800G /A  and Systemic Lupus Erythematosus

Naeimi , S

Volume 24, Issue 3 (Jun 2016) JSSU 2016, 24(3): 251-260 | Back to browse issues page

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Naeimi S. TGF-β1 Gene Polymorphism at Position -800G /A and Systemic Lupus Erythematosus. JSSU 2016; 24 (3) :251-260
URL: http://jssu.ssu.ac.ir/article-1-3626-en.html

TGF-β1 Gene Polymorphism at Position -800G /A and Systemic Lupus Erythematosus

S Naeimi ^*

Abstract: (5996 Views)

Introduction: Systemic lupus erythematosus (SLE) is a chronic systemic inflammatory autoimmune disease characterized by a breakdown of self-tolerance. Transforming growth factor-β1 is a cytokine produced by both immune and non immune cells, and it has a wide operating range. human TGF-β1 gene is located on chromosome 19q13 . The aim of this study was investigating the TGF-β1 Gene Polymorphism at Position -800G /A and Systemic Lupus Erythematosus the possible difference in two promoter polymorphisms of the transforming growth factor-β1 (TGF-β1) gene (-800G / A, -509C / T).

Methods: In this case - control study, a total of 150 patients with SLE and 150 healthy subjects were examined. DNA was extracted by saluting out method and Single nucleotide Polymorphisms of the TGF-β1gene were analyzed by the PCR-RFLP method and the .Data were compared in both groups by using Pearson’s chi-square and Hardy-weinberg equilibrium test.

Results: There was a statistically significant difference in AA genotype and A allele frequency distributions between SLE patients and the control group for the -800G / A polymorphism of the TGF-β1 gene (P < 0.05). At position -509, there was no statically significant difference in genotype and allele frequency between the patients and the control subjects.

Conclusion : The results of our study indicate that TGF-β1 gene promoter polymorphisms at positions -800 G/A maybe discuss susceptibility to SLE in southern Iranian patients.

Keywords: SLE, TGF-β1, Genotype, Polymorphism

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Type of Study: Original article | Subject: Immunology
Received: 2016/02/20 | Accepted: 2016/06/5 | Published: 2016/08/9

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