Evaluation and comparison of physicochemical properties, cytotoxicity and the ability of miRNA loading of different  cationic liposomes for gene therapy application

Nikoonahad Lotfabadi, Narges; Mohseni Kouchesfehani, Homa; Sheikhha, Mohammad Hasan; Kalantar, Seyed Mehdi

Volume 25, Issue 6 (Aug 2017) JSSU 2017, 25(6): 444-456 | Back to browse issues page

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Nikoonahad Lotfabadi N, Mohseni Kouchesfehani H, Sheikhha M H, Kalantar S M. Evaluation and comparison of physicochemical properties, cytotoxicity and the ability of miRNA loading of different cationic liposomes for gene therapy application. JSSU 2017; 25 (6) :444-456
URL: http://jssu.ssu.ac.ir/article-1-4299-en.html

Evaluation and comparison of physicochemical properties, cytotoxicity and the ability of miRNA loading of different cationic liposomes for gene therapy application

Narges Nikoonahad Lotfabadi

, Homa Mohseni Kouchesfehani ^*

, Mohammad Hasan Sheikhha

, Seyed Mehdi Kalantar

Abstract: (4890 Views)

Introduction: In the present study, various formulations of cationic liposomes were designed and prepared using different cationic lipids. It was performed to assess the physicochemical properties, miRNA loading ability and cellular toxicity rates of liposomes in order to use in gene therapy.
Methods: Different cationic liposome formulations (F1-F4) containing various cationic lipids, DOTAP, DOTMA, DOAB and DDAB with DPPC, cholesterol and phospholipid DSPE-mPEG were synthesized. Prepared nanoparticles were evaluated in term of particle size, polydisparity index, surface charge and cytotoxicity for 48 and 72 h in two cell lines. By using gel electrophoresis, the ability of synthesized cationic liposomes to entrap miRNA was also compared.
Results: All formulations were mono-dispersed. The particle size in F1, which contained DOTAP was lower than others (F2-F4) and its surface charge was more than them. Cationic liposomes based on DOTAP had no significant cytotoxicity as compared to other formulations. Also, F1 formulation was more capable to entrap miRNA than other formulas.
Conclusion: DOTAP-based cationic liposomes can be used efficiently in the gene therapy process, especially for the transfer of miRNA as a new therapeutic agent in cancer therapy

Keywords: miRNA, liposome, cytotoxicity, gene therapy

Full-Text [PDF 840 kb] (1575 Downloads)

Type of Study: Original article | Subject: Biology
Received: 2017/08/21 | Accepted: 2017/09/16 | Published: 2017/10/25

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