Volume 33, Issue 9 (12-2025)
JSSU 2025, 33(9): 9386-9400 |
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Ethics code: IR.ARAKU.REC.1401.128
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Shariat Zadeh S M A, Emadi M, Moghadasi S. Study of the Stereological and Biochemical Effects of L-Carnitine on Testicular Tissue after Toxicity Induction by Silver Nanoparticles in Mice. JSSU 2025; 33 (9) :9386-9400
URL: http://jssu.ssu.ac.ir/article-1-6371-en.html
URL: http://jssu.ssu.ac.ir/article-1-6371-en.html
Abstract: (27 Views)
Introduction: This study aimed to examine how L-carnitine's antioxidant properties could counteract the oxidative stress caused by silver nanoparticles, specifically looking at their harmful effects on rat testicular tissue.
Methods: In this experimental study involved 24 adult male NMRI mice that weighed 32±2 grams each. They were divided into 4 groups of 6 mice in each group. The groups were as followed: a control group, silver nanoparticles group (500 mg/body weight/day), L-carnitine group (100 mg/kg body weight/day), and silver nanoparticles and L-carnitine treatment group. Following 35 days of treatment administered via gavage, the mice were dissected, serum and sperm samples were prepared. Body and testicular weight, motility, sperm count, sperm viability and morphology, testosterone levels, and total antioxidant capacity and malondialdehyde levels were evaluated. Histopathological examination of the testicles was performed using hematoxylin-eosin staining. The number of spermatogonia, spermatocytes, spermatids and Sertoli cells was also reported. Finally, the significance level was considered p<0.05 and analyzed using one-way SPSS-ANoVA and Tukey statistical tests.
Results: Silver nanoparticles had no effect on the weight of the mice and exhibited no significant change (p>0.05). The L-carnitine-treated group showed a notable decrease in weight compared to the control group (p<0.05). Sperm parameters in the silver nanoparticles group showed a significant decrease compared to the control group (p<0.05). The total testicular volume and seminiferous tubule volume did not show significant differences in the experimental groups (p>0.05). The volume of interstitial tissue in the silver nanoparticles group significantly increased compared to the control group, while a significant decrease was observed in the silver nanoparticles and L-carnitine simultaneous treatment group compared to the silver nanoparticles group (p<0.05).
Conclusion: L-carnitine, as a natural antioxidant, prevented damage to the male reproductive system and testicular tissue induced by silver nanoparticles by reducing oxidative stress.
Methods: In this experimental study involved 24 adult male NMRI mice that weighed 32±2 grams each. They were divided into 4 groups of 6 mice in each group. The groups were as followed: a control group, silver nanoparticles group (500 mg/body weight/day), L-carnitine group (100 mg/kg body weight/day), and silver nanoparticles and L-carnitine treatment group. Following 35 days of treatment administered via gavage, the mice were dissected, serum and sperm samples were prepared. Body and testicular weight, motility, sperm count, sperm viability and morphology, testosterone levels, and total antioxidant capacity and malondialdehyde levels were evaluated. Histopathological examination of the testicles was performed using hematoxylin-eosin staining. The number of spermatogonia, spermatocytes, spermatids and Sertoli cells was also reported. Finally, the significance level was considered p<0.05 and analyzed using one-way SPSS-ANoVA and Tukey statistical tests.
Results: Silver nanoparticles had no effect on the weight of the mice and exhibited no significant change (p>0.05). The L-carnitine-treated group showed a notable decrease in weight compared to the control group (p<0.05). Sperm parameters in the silver nanoparticles group showed a significant decrease compared to the control group (p<0.05). The total testicular volume and seminiferous tubule volume did not show significant differences in the experimental groups (p>0.05). The volume of interstitial tissue in the silver nanoparticles group significantly increased compared to the control group, while a significant decrease was observed in the silver nanoparticles and L-carnitine simultaneous treatment group compared to the silver nanoparticles group (p<0.05).
Conclusion: L-carnitine, as a natural antioxidant, prevented damage to the male reproductive system and testicular tissue induced by silver nanoparticles by reducing oxidative stress.
Type of Study: Original article |
Subject:
Biology
Received: 2025/01/23 | Accepted: 2025/07/7 | Published: 2025/12/6
Received: 2025/01/23 | Accepted: 2025/07/7 | Published: 2025/12/6
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