Volume 32, Issue 6 (9-2024)                   JSSU 2024, 32(6): 7922-7932 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nasratipour R, Mirzaei M, Mehrabi M R. Examining the Frequency of Selected Genes Involved in Biofilm Formation in Clinical Isolates of Acinetobacter Baumannii. JSSU 2024; 32 (6) :7922-7932
URL: http://jssu.ssu.ac.ir/article-1-6178-en.html
Abstract:   (143 Views)
Introduction: Acinetobacter baumannii, a non-fermenting Gram-negative coccobacillus, exhibits high resistance to antimicrobial compounds. Biofilm formation is a crucial feature in many Acinetobacter species, contributing to their antibiotic resistance. The present study aimed to investigate the prevalence of ompA, csuA, bap and pgaB genes in clinical isolates of Acinetobacter baumannii with biofilm formation ability.
Methods: In this cross-sectional study, 49 isolates of Acinetobacter baumannii were collected from patients hospitalized in the health centers of Borujerd City. Acinetobacter baumannii isolates were confirmed by biochemical tests. In these isolates, the biofilm production ability was investigated by microtitreplate method. Then, using PCR method and specific primers, ompA, csuA, bap and pgaB genes were identified.The collected data were analyzed descriptively and analytically using SPSS version 16 software. Data analysis was done with Chi-square test, Fisher's exact test, and P<0.05 was considered as the basis of significance.
Results: The presence of ompA, csuA, bap, and pgaB genes was detected in 87%, 92%, 98%, and 100% of the isolates, respectively. Additionally, the microtitreplate method revealed that biofilm formation was strong in 3 isolates (6%), moderate in 17 isolates (35%), and weak in 29 isolates (59%).
Conclusion: The prevalence of genes associated with biofilm formation in Acinetobacter baumannii isolates was high. This suggests that the studied isolates possess a significant ability to form biofilm structures.
 
Full-Text [PDF 861 kb]   (82 Downloads)    
Type of Study: Original article | Subject: Biology
Received: 2024/02/21 | Accepted: 2024/05/29 | Published: 2024/09/5

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | SSU_Journals

Designed & Developed by : Yektaweb