Effect of endurance and high intensity interval swimming training on cardiac hypertrophy of male rats

Gharaat, Mohammad; Kashef, Majid; jameie, Behnam; Rajabi, Hamid

Volume 26, Issue 4 (Jun 2018) JSSU 2018, 26(4): 306-318 | Back to browse issues page

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Gharaat M, Kashef M, jameie B, Rajabi H. Effect of endurance and high intensity interval swimming training on cardiac hypertrophy of male rats. JSSU 2018; 26 (4) :306-318
URL: http://jssu.ssu.ac.ir/article-1-4493-en.html

Effect of endurance and high intensity interval swimming training on cardiac hypertrophy of male rats

Mohammad Gharaat ^*

, Majid Kashef

, Behnam Jameie

, Hamid Rajabi

Abstract: (4013 Views)

Introdution: Physical training causes functional and morphological changes in myocardium in response to regulatory adaptations same as Phosphatidilinositol-3-kinase (PI3K) changes. Present study aimed to experimentally investigate the effects of endurance and high intensity interval training on PI3K, structure and function of heart.
Methods: 24 adultmale rats (weight 221.6 ± 11.4 grams) divided randomly into control, sham, interval and endurance groups(n=6). The endurance group swam for 12 weeks/5 days per week. Also, the interval group swam 12 weeks/4 days per week. Twenty-four hours after the last exercise, echocardiography was done and 48h later, heart weight and left ventricle weight, and plasma for PI3K measurement (using ELAISA Reader) was taken. To assess the data, one-way ANOVA was utilized and Tukey-HSD was used to point out the place of significancy (α ≤ 0.05).
Results: Findings showed that heart (p= 0.03) and left ventricle weights (p= 0.01), stroke volume (0.01), Left ventricular end diastolic volume (0.04), and ejection fraction (p= 0.02) after endurance and high intensity interval training were significantly more than the sham and control groups (p< 0.05). In addition, PI3K significantly increased (p= 0.00) in the endurance (6.58 ng/ml) and interval (6.67) rather than the sham (5.37) and control groups (4.07).
Conclusion: It can be concluded that both the endurance and interval training schedules increase PI3K and they led to physiological hypertrophy of heart. Since the whole time of high intensity interval training is significantly shorter than that of the endurance training (~ 9%), it seems more beneficial to be executed.

Keywords: Physiological Hypertrophy, Endurance Training, Interval Training, PI3K, Wistar rat

Full-Text [PDF 1411 kb] (1453 Downloads)

Type of Study: Original article | Subject: Exercise Physiology
Received: 2018/03/12 | Accepted: 2018/05/10 | Published: 2018/09/15

References

1. McMullen JR, Shioi T, Zhang L, Tarnavski O, Sherwood MC, Kang PM, Izumo S. Phosphoinositide 3-kinase(p110alpha) plays a critical role for the induction of physiological, but not pathological, cardiac hypertrophy. Proc Natl Acad Sci U S A 2003; 100: 12355-60.

2. Pluim BM, Zwinderman AH, van-der-Laarse A, van-der-Wall EE. The athlete’s heart: a meta-analysis of cardiac structure and function. Circulation 2000; 101(3): 336-44.

3. McMullen J, Genings GL. Differences between pathological and physiological cardiac hypertrophy: novel therapeutic strategies to treat heart failure. Clin Exp Pharmacol Physiol 2007; 34(4): 255-62.

4. Venckunas T, Lionikas A, Marcinkeviciene JE, Raugaliene R, Alekrinskis A and Stasiulis A. Echocardiographic parameters in athletes of different sports. J Sports Sci Med 2008; 7(1): 151-56.

5. Pellicia A, Maron BJ, Spataro A, Proschan MA, Spirito P. The upper limit of physiologic hypertrophy in highly trained elite athletes. N Engl J Med 1991; 324(5): 295-301.

6. Wisloff U, Stoylen A, Loennechen JP, Bruvold M, Rognmo O, Haram PM, et al. Superior cardiovascular effect of aerobic interval training versus moderate continuous training in heart failure patients: A randomized study. Circulation 2007; 115(4): 3086-94.

7. Fathi M, Gharakanlou R, Abroun S, Mokhtari-Dizaji M and Rezaei R. The evaluation of cardiac changes following endurance training in male Wistar rats. Yafteh 2014; 15(5): 112-23. [Persian]

8. Kaplan ML, Cheslow Y, Vikstrom K, Malhotra A, Geenen DL, Nakouzi A, et al. Cardiac adaptations to chronic exercise in mice. Am J Physiol 1994; 267(3): H1167–H1173.

9. Gharaat MA, Kashef M, Jameie B, Rajabi H. Effect of endurance and high intensity interval swimming training on cardiac structure and Hand2 expression of rats. J Shahid Sadoughi Uni Med Sci 2017; 25(9): 748-58. [Persian]

10. Holloway TM, Bloemberg D, da Silva ML, Simpson JA, Quadrilatero J, Spriet LL. High intensity interval and endurance training have opposing effects on markers of heart failure and cardiac remodeling in hypertensive rats. PLoS ONE 2015; 10(3): 341-51.

11. Vanhaesebroeck B, Leevers SJ, Panayotou G, Waterfi eld MD. Phosphoinositide 3-kinases: a conserved family of signal transducers. Trends Biochem Sci 1997; 22: 267–72.

12. Luo J, McMullen JR, Sobkiw CL, Zhang L, Dorfman AL, Sherwood MC, Et al. Class IA phosphoinositide 3-kinase regulates heart size and physiological cardiac hypertrophy. Mol Cell Biol 2005; 25(21): 9491-502.

13. Shioi T, Kang PM, Douglas PS, Hampe J, Yballe CM, Lawitts J, et al. The conserved phosphoinositide 3-kinase pathway determines heart size in mice. EMBO 2000; 19(11): 2537–48.

14. Hildick-Smith DJ, Shapiro LM. Echocardiographic differentiation of pathological and physiological left ventricular hypertrophy. Heart 2001; 85(6): 615–19.

15. Sheykhlouvand M, Khalili E, Agha-Alinejad H, Gharaat MA. Hormonal and physiological adaptations to high-intensity interval training in professional male canoe polo athletes. J Str & Condit Res 2016; 30(3): 859–66.

16. Gillen JB, Gibala MJ. Is high-intensity interval training a time-efficient exercise strategy to improve health and fitness? Appl. physiol. nutria. Metabolism 2014; 39(3): 409-12.

17. Fathi M, Gharakhanlou R. Effect of Endurance training on Hand2 gene expression in left ventricle of male rats. Sport Physiology 2015; 7(25): 57-68. [Persian]

18. Terada S, Tabata I, Higuchi M. Effect of high intensity intermittent swimming training of fatty acid oxidation enzyme activity in rat skeletal muscle. Jpn J Physiol 2004; 54(1): 47-52.

19. De Rocha GL, Crisp AH, de Oliveira MRM, da Silva CA, Silva JO, Duarte AGO, et al. Effect of High Intensity Interval and Continuous Swimming Training on Body Mass Adiposity Level and Serum Parameters in High-Fat Diet Fed Rats. ScientificWorld J 2016; 2016: 1-8.

20. Bernardo BC, Weeks KL, Pretorius L, McMullen JR. Molecular distinction between physiological and pathological cardiac hypertrophy: Experimental findings and therapeutic strategies. Pharmacol & Therapeut 2010; 128: 191-227.

21. O’Neill BT, Kim J, Wende AR, Theobald HA, Tuinei J, Buchanan J. A Conserved Role for Phosphoinositide-3-Kinase but Not Akt Signaling in Mitochondrial Adaptations that Accompany Physiological Cardiac Hypertrophy. Cell Metab- 2007; 6(4): 294-306.

22. Medeiros A, Oliveira EM, Gianolla R, Casarini DE, Negrão CE, Brum PC. Swimming training increases cardiac vagal activity and induces cardiac hypertrophy in rats. Brazilian J Med Biol Res 2004; 37(12): 1909-17.

23. Da Silva JND, Fernandes T, Soci UPR, Monteiro AWA, Phillips MI, De Oliveira EM. Swimming training in rats increases cardiac MicroRNA-126 expression and angiogenesis. Med Sci Sports Exerc 2012; 44(8): 1453-62.

24. Kaplan ML, Cheslow Y, Vikstrom K, Malhotra A, Geenen DL, Nakouzi A. Cardiac adaptations to chronic exercise in mice. Am J Physiol 1994; 267(3): H1167-73.

25. Mihl C, Dassen WR, Kuipers H. Cardiac remodelling: concentric versus eccentric hypertrophy in strength and endurance athletes. Neth Heart J 2008; 16(4): 129-33.

26. Morganroth J, Maron BJ, Henry WL, Epstein SE. Comparative left ventricular dimensions in trained athletes. Ann Intern Med 1975; 82(4): 521-24.

27. Midgley AW, McNaughton LR, Carroll S. Physiological determinants of time to exhaustion during intermittent treadmill running at VO2max. Int J Sports Med 2007; 28(4): 273-80.

28. Kemi OJ, Haram PM, Loennechen JP. Moderate vs. high exercise intensity: differential effects on aerobic fitness, cardiomyocyte contractility, and endothelial function. Cardiovasc Res 2005; 67(1): 161-72.

29. Sheykhlouvand M, Gharaat MA, Khalili E, Agha-Alinejad H. The effect of high-intensity interval training on ventilatory threshold and aerobic power in well-trained canoe polo athletes. Sci & Sports 2016; 31(5): 283-89.

30. Hayward R, Lien CY. Echocardiographic Evaluation of Cardiac Structure and Function during Exercise Training in the Developing Sprague–Dawley Rat. J Am Associate for Lab Animal Sci 2011; 50(4): 454-61.

31. Groban L, Jobe H, Lin M, Houle T, Kitzman DA and Sonntag W. Effects of short-term treadmill exercise training or growth hormone supplementation on diastolic function and exercise tolerance in old rats. J Gerontol a Biol Sci Med Sci 2008; 63(9): 911–20.

32. Rottman JN, Ni G, Khoo M, Wang Z, Zhang W, Anderson ME. Temporal changes inventricular function assessed echocardiographically in conscious and anesthetized mice. J Am Soc Echocardio 2003; 16(11): 1150-57.

33. McMullen J, Shioi T, Huang W, Zhang L, Tarnavski O, Bisping E, et al. The Insulin-like Growth Factor 1 Receptor Induces Physiological Heart Growth via the Phosphoinositide 3-Kinase(p110α) Pathway. J of Biol Chem 2003 279: 4782-93.

34. Weeks KL, Gao X, Du XJ, Boey EJ, Matsumoto A, Bianca CB, et al. PI3K(p110α) Is a Master Regulator of Exercise-Induced Cardioprotection and PI3K Gene Therapy Rescues Cardiac Dysfunction. Circ Heart Fail 2012; 5(4): 523-34.