Effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats

Dashti khavidaki, Mohammad Hassan; Azamian Jazi , Akbar; Banitalebi, Ebrahim; Faramarzi , Mohammad

Volume 26, Issue 1 (Apr 2018) JSSU 2018, 26(1): 85-96 | Back to browse issues page

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Dashti khavidaki M H, Azamian Jazi A, Banitalebi E, Faramarzi M. Effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats . JSSU 2018; 26 (1) :85-96
URL: http://jssu.ssu.ac.ir/article-1-4358-en.html

Effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats

Mohammad Hassan Dashti khavidaki ^*

, Akbar Azamian Jazi

, Ebrahim Banitalebi

, Mohammad Faramarzi

Abstract: (5215 Views)

IntrodutionThe purpose of this study was investigating the effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats.
Methods: 24 male Wistar rats were randomly assigned to three groups of eight, including diabetic group with endurance exercise (D-E), diabetic (D) and healthy control groups (Con). After induction of diabetic rats by injection streptozotocin was administered intraperitoneal , endurance exercise was applied for eight weeks, three sessions pre week in diabetic rats. Exercise intensity was equal to a speed of 21-25 m / min. The relative expression of CGI-58 and ATGL protein was measured with western blot technique and serum insulin and glucose levels were measured with a specialized kit. One-way ANOVA test was performed using SPSS-20 software and at a significance level less than 5%.
Results: Results showed that ATGL and CGI-58 values were significantly different between the three groups (p <0.001). ATGL difference between the groups of diabetic group with endurance exercise with control (p = 0.001) and diabetic (p = 0.001)was significant. CGI-58 difference between the groups of diabetic group with endurance exercise with control (p = 0.001) and diabetic (p = 0.002) was significant. In addition, serum glucose and insulin levels decreased significantly after eight weeks of training (p <0.05).
Conclusion: It seems that CGI-58 play a vital role in activating lipolysis by ATGL and increasing in CGI-58 leads to an increase in ATGL and ultimately leads to increased levels of intramuscular triglyceride oxidation and improved insulin resistance.

Keywords: CGI-58, ATGL, endurance exercise, insulin, glucose

Full-Text [PDF 1262 kb] (1490 Downloads)

Type of Study: Original article | Subject: Exercise Physiology
Received: 2017/10/16 | Accepted: 2017/12/30 | Published: 2018/05/29

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