Volume 24, Issue 11 (Feb 2017)                   JSSU 2017, 24(11): 924-937 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

torabi zarchi M, Mirhosseini M. Investigation of Combination Effect of Magnesium Oxide and Iron Oxide Nanoparticles on the Growth And Morphology of the Bacteria Staphylococcus Aureus and Escherichia Coli in Juice. JSSU. 2017; 24 (11) :924-937
URL: http://jssu.ssu.ac.ir/article-1-3861-en.html
Abstract:   (5973 Views)

Introduction: Nanoparticles (NPs) are one of the antibacterial substances, among them nanoparticles type MgO and Fe2O3 are less toxic to mammalian cells. So, the aim of this study was investigation of combination effects of iron oxide and magnesium oxide nanoparticles on the growth of Staphylococcus aureus and Escherichia coli (E.coli) to achieve the optimum combination of nanoparticles inhibit the growth of Staphylococcus aureus and Escherichia coli in food (juice).

Methods: In this experimental research, the effect of MgO and Fe2O3 Nanoparticles compound on Staphylococcus aureus and Escherichia coli bacteria in liquid environment was investigated, and then their effect was investigated separately in juices of carrot, pomegranate and apple via colony count approach. Also, scanning electron microscopy was used to characterize the morphological changes of Staphylococcus aureus and Escherichia coli after antimicrobial treatments. The results of the research were analyzed using one way ANNOVA.

Results: The results of the research indicated that in liquid medium, these nanoparticles lead to reduce the growth of both bacteria. compound of 1.5Mg+0.5Fe2O3 was introduced as the most appropriate antibacterial compounds; Staphylococcus aureus sensitivity to Escherichia coli was higher against nanoparticles. The findings of research about the juices revealed that the combined effect of nanoparticles reduced the growth of both bacteria. the combined effect of Fe2o3 and MgO nanoparticles treatments distorted and damaged the cell membrane, resulting in a leakage of intracellular contents and eventually the death of bacterial cells.

Conclusion: Nanoparticles in the allowed concentrations have significant effect on Staphylococcus aureus and Escherichia coli bacteria.

Full-Text [PDF 1033 kb]   (2761 Downloads)    
Type of Study: Original article | Subject: Microbiology
Received: 2016/08/28 | Accepted: 2016/12/10 | Published: 2017/04/24

1. Yang W, Li H, Gong Y, Chen W, Gaidau C. Preparation of silver nanoparticles of enhanced antibacterial effect with benzalkonium bromide. Journal of Optoelectronics and Advanced Materials.2011;13(5):661
2. Hoseinzadeh E, Alikhani M, Samarghandy M. Evaluation of Synergistic Effect of Commercial Zinc Oxide and Copper Oxide Nanoparticles against Gram Positive and Gram Negative Bacteria by Fraction Inhibitory Concentration Index. ZUMS Journal.2012; 20(82):29-41
3. Hadi M, Shokoohi R, Ebrahimzadeh Namvar A, Karimi M, Solaimany Aminabad M. Antibiotic resistance of isolated bacteria from urban and hospital wastewaters in Hamadan City. Iranian Journal of Health and Environment. 2011; 4(1):105-14
4. Kalantar E, Maleki A, Khosravi M, Mahmodi S. Evaluation of UltrasoundWaves Effect on Antibiotic Resistance Pseudomonas Aeruginosa and Staphylococcus Aureus Isolated from Hospital and their Comparison with Standard Species. Iranian Journal of Health and Environment.2010; 3(3):319-26
5. Tawale JS, Dey KK, Pasricha R, Sood KN, Srivastava AK. Synthesis and characterization of ZnO tetrapods for optical and antibacterial applications. Thin Solid Films. 2010;519(3):1244-7.
6. Zhang L, Ding Y, Povey M, York D. ZnO nanofluids – A potential antibacterial agent. Progress in Natural Science.2008;18(8):939-44
7. Jones GL, Muller CT, O'Reilly M, Stickler DJ. Effect of triclosan on the development of bacterial biofilms by urinary tract pathogens on urinary catheters. The Journal of antimicrobial chemotherapy. 2006;57(2): 266-72
8. Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA, Syed AF. Metal-based nanoparticles and their toxicity assessment. Wiley interdisciplinary reviews Nanomedicine and nanobiotechnology 2010; 2(5):544-68.
9. Heinlaan M, Ivask A, Blinova I, Dubourguier HC, Kahru A. Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus. Chemosphere. 2008; 71(7):1308-16.
10. Jeng HA, Swanson J. Toxicity of Metal Oxide Nanoparticles in Mammalian Cells. Journal of Environmental Science and Health, Part A. 2006;41(12):2699-711.
11. Espitia P, Soares Nd, Teófilo R, Vitor D, Coimbra Jd, de Andrade N, et al. Optimized dispersion of ZnO nanoparticles and antimicrobial activity against foodborne pathogens and spoilage microorganisms. J Nanopart Res.2013;15(1): 1-16.
12. Tayel AA, El-Tras WF, Moussa S, El-Baz AF, Mahrous H, Salem MF, et al. Antibacterial action of zinc oxide nanoparticles against foodborne pathogens. Journal of Food Safety 2011;31(2):211-8.
13. Jin T, He Y. Antibacterial activities of magnesium oxide (MgO) nanoparticles against foodborne pathogens. J Nanopart Res.2011; 13(12):6877-85.
14. Shi L-E, Xing L, Hou B, Ge H, Guo X, Tang Z. Inorganic nano mental oxides used as anti-microorganism agents for pathogen control. Current Research, Technology and Education Topics in Applied Microbiology and Microbial.2010: 361-68.
15. Krishnamoorthy K, Manivannan G, Kim SJ, Jeyasubramanian K, Premanathan M. Antibacterial activity of MgO nanoparticles based on lipid peroxidation by oxygen vacancy. J Nanopart Res 2012; 14:1063.
16. Di D-R, He Z-Z, Sun Z-Q, Liu J. A new nano-cryosurgical modality for tumor treatment using biodegradable MgO nanoparticles. Nanomedicin 2012; 8(8): 1233-41.
17. Rezaei-Zarchi S, Javed A, Javeed Ghani M, Soufian S, Barzegari Firouzabadi F, Bayanduri Moghaddam A, et al. Comparative Study of Antimicrobial Activities of TiO2 and CdO Nanoparticles against the Pathogenic Strain of Escherichia coli. Iranian Journal of Pathology 2010;5(2):83-9.
18. Kawata K, Osawa M, Okabe S. In Vitro Toxicity of Silver Nanoparticles at Noncytotoxic Doses to HepG2 Human Hepatoma Cells. Environmental Science & Technology 2009;43(15):6046-51
19. Barati B., Saadati M., Bahmani M. Kh. Isolation and Detection of Enterotoxigenic Staphylococcus Aureus Type A by Multiplex PCR. Journal of Military Medicine 2006; 8(2):119-28.
20. Shirzad Siboni M, Samadi M, Yang J, Lee S. Photocatalytic reduction of Cr (VI) and Ni (II) in aqueous solution by synthesized nanoparticle ZnO under ultraviolet light irradiation: a kinetic study. Environmental technology 2011;32(14):1573-9
21. Parente E, Brienza C, Moles M, Ricciardi A. A comparison of methods for the measurement of bacteriocin activity. Journal of Microbiological Methods 1995;22(1):95-108.
22. Mirhosseini M, Firouzabadi FB. Antibacterial activity of zinc oxide nanoparticle suspensions on food-borne pathogens. International Journal of Dairy Technology 2013; 66(2):291-5.
23. Lin D, Xing B. Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environmental Pollution 2007; 150(2):243-50.
24. Chung Y-C, Su Y-P, Chen C-C, Jia G, Wang H-l, Wu JG, et al. Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. Acta Pharmacologica Sinica 2004;6:932
25. Sinha R, Karan R, Sinha A, Khare SK. Interaction and nanotoxic effect of ZnO and Ag nanoparticles on mesophilic and halophilic bacterial cells. Bioresource Technology 2011; 102(2):1516-20.
26. Kalishwaralal K, BarathManiKanth S, Pandian SRK, Deepak V, Gurunathan S. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids and Surfaces B: Biointerfaces 2010;79(2):340-4.
27. Te Dorsthorst D, Verweij P, Meis J, Punt N, Mouton J. Comparison of fractional inhibitory concentration index with response surface modeling for characterization of in vitro interaction of antifungals against itraconazole-susceptible and-resistant Aspergillus fumigatus isolates. Antimicrobial agents and chemotherapy. 2002; 46(3):702-7.
28. Reddy KM, Feris K, Bell J, Wingett DG, Hanley C, Punnoose A. Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Applied physics letters 2007;90(21): 213902.
29. Ren G, Hu D, Cheng EWC, Vargas-Reus MA, Reip P, Allaker RP. Characterisation of copper oxide nanoparticles for antimicrobial applications. International Journal of Antimicrobial Agents 2009;33(6):587-90.
30. Mandal S, Pal NK, Chowdhury IH, Debmandal M. Antibacterial Activity of Ciprofloxacin and Trimethoprim, Alone and in Combination, Against Vibrio cholerae O 1 Biotype El Tor Serotype Ogawa Isolates. Polish Journal of Microbiology 2009;58(1):57-60.
31. Jin T, Sun D, Su JY, Zhang H, Sue HJ. Antimicrobial Efficacy of Zinc Oxide Quantum Dots against Listeria monocytogenes, Salmonella Enteritidis, and Escherichia coli O157:H7. Journal of Food Science. 2009;74(1):M46-52.
32. Sekhon BS. Food nanotechnology–an overview. Nanotechnology, science and applications 2010;3: 1-15.
33. Feng Q, Wu J, Chen G, Cui F, Kim T, Kim J. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of biomedical materials research 2000;52(4):662-8.
34. Zhu M-T, Feng W-Y, Wang B, Wang T-C, Gu Y-Q, Wang M, et al. Comparative study of pulmonary responses to nano-and submicron-sized ferric oxide in rats. Toxicology 2008;247(2):102-11.
35. Singh M, Singh S, Prasad S, Gambhir I. Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Journal of Nanomaterials and Biostructures 2008;3(3):115-22.
36. Allahverdiyev AM, Kon KV, Abamor ES, Bagirova M, Rafailovich M. Coping with antibiotic resistance: combining nanoparticles with antibiotics and other antimicrobial agents. Expert Rev Anti Infect Ther 2011; 9(11): 1035-52
37. Chaudhry Q, Scotter M, Blackburn J, Ross B, Boxall A, Castle L, et al. Applications and implications of nanotechnologies for the food sector. Food Additives & Contaminants: Part A 2008;25(3):241-58.
38. Bouwmeester H, Dekkers S, Noordam MY, Hagens WI, Bulder AS, de Heer C, et al. Review of health safety aspects of nanotechnologies in food production. Regulatory Toxicology and Pharmacology 2009; 53(1):52-62.
39. Mirhosseini M, Afzali M. Investigation into the antibacterial behavior of suspensions of magnesium oxide nanoparticles in combination with nisin and heat against Escherichia coli and Staphylococcus aureus in milk. Food Control 2016; 15(68):208.
40. Trumbo P, Yates AA, Schlicker S, Poos M. Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. Journal of the American Dietetic Association 2001;101(3): 294-301.

Add your comments about this article : Your username or Email:

Send email to the article author

© 2021 CC BY-NC 4.0 | SSU_Journals

Designed & Developed by : Yektaweb