Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods

Geranmayeh, Arghavan; Hoseini, Farzaneh; Rafiei Tabatabaei, Robab

Volume 25, Issue 7 (oct 2017) JSSU 2017, 25(7): 564-571 | Back to browse issues page

Mendeley

Zotero

RefWorks

Geranmayeh A, Hoseini F, Rafiei Tabatabaei R. Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods . JSSU 2017; 25 (7) :564-571
URL: http://jssu.ssu.ac.ir/article-1-3765-en.html

Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods

Arghavan Geranmayeh

, Farzaneh Hoseini ^*

, Robab Rafiei Tabatabaei

Abstract: (4357 Views)

Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method.
Methods: In this cross-sectional study, 60 Salmonella enterica serovar Typhimurium were obtained from the human fecal samples. These strains were identified by standard microbiological and biochemical tests. Then, ERIC-PCR and BOX-PCR were carried out for determination of molecular relatedness of Salmonella enterica serovar Typhimurium using specific primers.
Results: The results showed that all 60 Salmonella enterica serovar Typhimurium were separable using ERIC 1, ERIC 2 and BOXAIR. In electrophoresis, 2-11 bands with 20-3200bp for ERIC-PCR and 2-10 bands with 200-1500bp for BOX-PCR were observed. Therefore, 13 different clusters (C1-C13) in ERIC-PCR and 21 different clusters (C1-C21) in BOX-PCR were identified.
Conclusion: The results showed that Salmonella enterica serovar Typhimurium strains were non-homolog. Therefore, ERIC-PCR and BOX-PCR methods are appropriate methods for molecular typing of Salmonella strains and determine the original infection source for the epidemiological survey as well as infection prevention program.

Keywords: Salmonella enterica serovar Typhimurium, BOX-PCR, ERIC-PCR.

Full-Text [PDF 914 kb] (1196 Downloads)

Type of Study: Original article | Subject: Microbiology
Received: 2016/06/10 | Accepted: 2016/12/24 | Published: 2017/11/15

References

1. Golberg D, Kroupitski Y, Belausov E, Pinto R, Sela S. Salmonella Typhimurium internalization is variable in leafy vegetables and fresh herbs. International j food microbiology 2011; 145(1): 250-7.

2. Williams C, McColl K. Review article: proton pump inhibitors and bacterial overgrowth. Alimentary pharmacology & therapeutics 2006; 23(1): 3-10.

3. Miao EA, Miller SI. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proceedings of the National Academy of Sci 2000; 97(13): 7539-44.

4. Woo Y, Lee S. Genetic diversity of multi-resistant Salmonella enterica serotype Typhimurium isolates from animals and humans. J microbiology-seoul 2006; 44(1): 106.

5. Nath G, Maurya P, Gulati AK. ERIC PCR and RAPD based fingerprinting of Salmonella Typhi strains isolated over a period of two decades. Infection, genetics and evolution 2010; 10(4): 530-6.

6. Anjali Tikoo†, A. K. Tripathi, S. C. Verma, N. Agrawal, Gopal Nat. Application of PCR fingerprinting techniques for identification and discrimination of Salmonella isolates. Current Sci 2001; 80(8): 1048-52.

7. Versalovic J, Koeuth T, Lupski R. Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomes. Nucleic acids research 1991; 19(24): 6823-31.

8. Albufera U, Bhugaloo-Vial P, Issack M, Jaufeerally-Fakim Y. Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis. Infection, genetics and evolution 2009; 9(3): 322-7.

9. Johnson JR, Clabots C. Improved Repetitive-Element PCR Fingerprinting ofSalmonella enterica with the Use of Extremely Elevated Annealing Temperatures. Clinical and diagnostic laboratory immunology 2000; 7(2): 258-64.

10. Fendri I, Hassena AB, Grosset N, Barkallah M, Khannous L, Chuat V, et al. Genetic diversity of food-isolated Salmonella strains through pulsed field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC-PCR). PloS one 2013; 8(12): e81315.

11. Karami A, Bagheri B, Ahmadi Z, Pourali F. Comparing Fluorescent Loop-Mediated Isothermal Amplification and PCR in Detecting Salmonella. J Mazandaran Uni of Medical Sci. [Research(Original)] 2012; 22(95): 48-55. [persion]

12. Chmielewski R, Wieliczko A, Kuczkowski M, Mazurkiewicz M, Ugorski M. Comparison of ITS Profiling, REP‐and ERIC‐PCR of Salmonella Enteritidis Isolates from Poland. J Veterinary Medicine, Series B 2002; 49(4): 163-8.

13. Rasschaert G, Houf K, Imberechts H, Grijspeerdt K, De Zutter L, Heyndrickx M. Comparison of five repetitive-sequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates. J clinical microbiology 2005; 43(8): 3615-23.

14. Saxena M, Singh V, Lakhcharua B, Taj G, Sharma B. Strain differentiation of Indian isolates of Salmonella by ERIC-PCR. Research in veterinary science 2002; 73(3): 313-4.

15. Van Belkum A, Sluijuter M, De Groot R, Verbrugh H, Hermans P. Novel BOX repeat PCR assay for high-resolution typing of Streptococcus pneumoniae strains. J clinical microbiology 1996; 34(5): 1176-9.

16. Tacão M, Alves A, Saavedra MJ, Correia A. BOX-PCR is an adequate tool for typing Aeromonas spp. Antonie van Leeuwenhoek 2005; 88(2): 173-9.

17. Lim H, Lee KH, Hong C-H, Bahk G-J, Choi WS. Comparison of four molecular typing methods for the differentiation of Salmonella spp. International journal of food microbiology 2005; 105(3): 411-8.

18. Oliveira SDd, Bessa MC, Santos LRd, Cardoso MRdI, Brandelli A, Canal CW. Phenotypic and genotypic characterization of Salmonella Enteritidis isolates. Brazilian j microbiology 2007; 38(4): 720-8.

19. Ashna J, Farook K, Ali A-Z. Molecular epidemiology analysis of Salmonella enterica serotype Typhi used Pulsed-Field Gel Electrophoresis (PFGE). J Biotechnology Research Center 2014; 8(2): 49-59.