Volume 25, Issue 7 (oct 2017)                   JSSU 2017, 25(7): 526-536 | Back to browse issues page

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Ahmadirad H, Hajizadeh M R H, Mahmoodi M, Mirzaee M, Shahrbabaki F M, Soltaninejad M. Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453). JSSU 2017; 25 (7) :526-536
URL: http://jssu.ssu.ac.ir/article-1-3717-en.html
Abstract:   (4542 Views)

Background: Breast cancer is one of the most common cancers among women.  Incorrect pattern of gene expression involved in epigenetic  Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.
  resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined  effect of  resveratrol on the expression of genes referred to the two classes of  breast cancer cell lines

Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression.

Results: The results showed that resveratrol decrease in gene expression APOBEC3B and  DNMT-1 And increased expression of  TET_1 in both of cell lines.  .

Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects  through  epigenetic mechanisms applied.  However, the final decision needs to be further investigation.

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Type of Study: Original article | Subject: Biochemistry
Received: 2016/05/9 | Accepted: 2017/03/11 | Published: 2017/11/13

1. Rajaee fard A, Tabatabaee H, Moghimi B, Zeighami B, Safaeii A, Tabeii Z. Checking Cases of esophageal cancer in Fars Province (2001-2006). J Kermanshah Medical Sciences university 2009; 13(1): 65-73. [persian]
2. Holt CL, Kyles A, Wiehagen T, Casey C. Development of a spiritually based breast Cancer educational booklet for African-American women. Cancer Control 2003; 10(5): 37-44.
3. Howell A , Sims AH, Ong KR, Harvie MN, Evans DG, Clarke RB, A et al. Mechanisms of Disease prediction and prevention of breast cancer cellular and molecular interactions. Nat Clin Pranct Oncol 2005; 2(12): 635-46.
4. Varangot M , Barrios E, Sóñora C, Aizen B, Pressa C, et al. Clinical evaluation of a panel FNA markers in the detection of disseminated tumor cells in patients with operable breast cancer. Oncol Rep 2005; 14(2): 537-45.
6. 5- Brunicardi FCh. Schwartz's principles of surgery. 8th ed. New York: McGraw-Hill 2005; 654-9.
7. Lehto US, Ojanen M, DybaiT, Aromaa A, kellokumpu-Lehtinen P. Baseline psychosocial predictors of survival in localised breast cancer. British J Cancer 2006; 94(9): 1245-52.
8. Burns MB, Lackey L, Carpenter MA, Rathore A, Land AM, Leonard B, et al. APOBEC3B is an enzymatic source of mutation in breast cancer. Nature 2013; 494(7437): 366-70.
9. Tan L, Shi YG. TET-1 family proteins and 5- hydroxymethylcytosine in development and disease. Development 2012; 139(11): 1895-902.
10. Chahwan R, Wontakal SN, Roa S. Crosstalk between genetic and epigenetic information through cytosine deamination. Trends in genetics 2010; 26(10): 443-8.
11. Lackey L, Demorest ZL, Land AM, Hultquist JF, Brown WL, Harris RS. APOBEC3B and AID have similar nuclear import mechanisms. J molecular biology 2012; 419(5): 301-14.
12. Ooms M, Krikoni A, Kress AK, Simon V, Münk C. APOBEC3A, APOBEC3B, and APOBEC3H haplotype 2 restrict human T- lymphotropic virus type 1. J virology 2012; 86(11): 6097-6108.
13. Almeida L, Custodio A, Pinto G, Santos M, Almeida J, Clara C, et al. Polymorphisms and DNA methylation of gene TP53 associated with extra-axial brain tumors. Genet Mol Res 2009; 8(1): 8-18.
14. Guo JU, Su Y, Zhong C, Ming G, Song H. Hydroxylation of 5-methylcytosine by TET-11 promotes active DNA demethylation in the adult brain. Cell 2011; 145(3): 423-34.
15. Popp C, Dean W, Feng S, Cokus SJ, Andrews S, Pellegrini M, et al. Genome-wide erasure of DNA methylation in mouse primordial germ cells is affected by AID deficiency. Nature 2010; 463(7284): 1101-5.
16. Yang H, Liu Y, Bai F, Zhang J, Ma S, Liu J, et al. Tumor development is associated with decrease of TET-1gene expression and 5-methylcytosine hydroxylation. Oncogene 2013; 32(5): 663-9.
17. Yang AS, Estécio MR, Doshi K, Kondo Y, Tajara EH, Issa JPJ. A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements. Nucleic acids research 2004; 32(3): 20-38.
18. Gordanian B, Behbahani M, Carapetian J, Fazilati M. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species. Research in Pharmaceutical Sci 2014; 9(2): 91-96.
19. Hoffman E. Cancer and the search for selective biochemical inhibitors. 2th ed. Boca Raton. CRC Press; 2007
21. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313-52.
22. Frankel EN, Waterhous AL, Kinsella JE. Inhbition of human LDL oxidation by resveratrol. Lancet 1993; 341(8852): 1103-4.
24. Wadi Poor M. Proceedings of the nurses in Isfahan 2009; 7(12): 76-8. [persion]
25. Xu Q, Si LY. Resveratrol role in cardiovascular and metabolic health and potential mechanisms of action. Nutr Res 2012; 32(9): 648-58.
26. Dong H, Ren H. New progression in the study of protecties of resveratrol in anticardiovascular disease. Bratisl Lek Listy 2004; 105(5-6): 225-29.
27. Lackey L, Demorest ZL, Land AM, Hultquist JF, Brown WL, Harris RS. APOBEC3B and AID have similar nuclear import mechanisms. J molecular biology 2012; 419(5): 301-14.
28. Abolhassani A, Riazi GH, Azizi E, Amanpour S, Muhammadnejad S, Haddadi M, et al. FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines. J Cancer 2014; 5(7): 537.
29. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, et al. Primer3 new capabilities and interfaces. Nucleic acids 2012; 40(15): e115.
30. Jaenisch R and Bird A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat Genet 2003; 33: 245-54.
31. Ward C, Langdon SP, Mullen P, Harris AL, Harrison DJ, Supuran CT, et al. New strategies for targeting the hypoxic tumour microenvironment in breast cancer. Cancer treatment reviews 2013; 39(2): 171-9.
32. Malekyian Fini E, Shavandi N, Saremi A. Effect of short-term Resvin supplementation on total antioxidant capacity ‚super oxide dismutase, and creatine kinase in elite women volleyball players. Iranian J Nutrition Sciences & Food Technology 2013; 8(3): 79-86.
33. Casanova F, Quarti J, da Costa DCF, Ramos CA, da Silva JL, Fialho E. Resveratro chemosensitize breast cancer cells to melphalan by cell cycle arrest. J cellular biochemistry 2012; 113(8): 2586-96.
34. Qin W, Zhu W, Sauter E. Resveratrol induced DNA methylation in ER+ breast cancer. AACR 2005; 46(9): 2750
35. Sieuwerts Am, Burns M, Look M, Meijer-Van Gelder M, Schlicker A, Heidemann M, et al. Abstract S6-05: High levels of APOBEC3B, a DNA deaminase and an enzymatic source of C-to-T transitions, are a validated marker of poor outcome in estrogen receptor-positive breast cancer. Cancer Res 2013;73(24 ); 60- 8.
36. Rhee I, Jair K-W, Yen R-WC, Lengauer C, Herman JG, Kinzler KW, et al. CpG methylation is maintained in human cancer cells lacking DNMT_1. Nature 2000; 404(6781): 1003-7.
37. Lee WJ, Shim J-Y, Zhu BT. Mechanisms for the inhibition of DNA methyltransferases by tea catechins and bioflavonoids. Molecular pharmacology 2005; 68(4): 1018-30.
38. Link A, Balaguer F, Goel A. Cancer chemoprevention by dietary polyphenols: promising role for epigenetics. Biochemical pharmacology 2010; 80(12): 1771-92.
39. Ito S, Shen L, Dai Q, Wu SC, Collins LB, Swenberg JA, et al. Tet proteins can convert 5-methylcytosine to 5- formylcytosine and 5-carboxylcytosine. Science 2011; 333(6047): 1300-3.
40. Tahiliani M, Koh KP, Shen Y, Pastor WA, Bandukwala H, Brudno Y, et al. Conversion of 5- methy cytosin to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 2009; 324(5929): 930-5.
41. Yang H, Liu Y, Bai F, Zhang J, Ma S, Liu J, et al. Tumor development is associated with decrease of TET-1 gene expressionand 5-methylcytosine hydroxylation. Oncogene 2013; 32(5): 663-9.

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