Volume 24, Issue 3 (Jun 2016)                   JSSU 2016, 24(3): 211-221 | Back to browse issues page

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Kolahi M, Tabandeh M, Saremy S, Hosseini S, Hashemitabar M. The Study of Apoptotic Effect of p-Coumaric Acid on Breast Cancer Cells MCF-7. JSSU 2016; 24 (3) :211-221
URL: http://jssu.ssu.ac.ir/article-1-3569-en.html
Abstract:   (10152 Views)

Introduction: Polyphenolic compounds have anti proliferative and induced apoptotic features on cancer cells. p-Coumaric acid can be abundantly found in fruits, vegetables, plant production and honey. .  Breast cancer is the most frequently diagnosed cancer among women in the world. This study aimed to investigate the effect and mechanism of p- coumaric acid on apoptosis of MCF-7 breast cancer cells.

Methods: In order to study appoptic effect of p- coumaric acid, MCF-7 breast cancer cells were treated with different concentrations of p- coumaric acid (10, 37, 70, 150 and 300 mM) for 24 h. Cell viability was determined using MTT assay. Apoptosis markers including phosphatidylserine exposure at the outer leaflet of the plasma membrane were measured using flow cytometery for Annexin V affinity.

Results: Cell viability of MCF-7 cells was decreased with increasing of p- coumaric acid concentration. Maximal effect of p- coumaric acid was observed in cells that treated with 300 mM for 24h (p< 0.05). Viability assay showed that the IC50 of p- coumaric acid in MCF-7 cells was about 40 mM. p- coumaric acid at dose of 300 mM significantly increased the late apoptotic cells with Annexin V+ and propium iodide (PI+) features after 24 h treatment.

Conclusion: The results of this study showed that p- coumaric acid had effective appoptic activity against MCF-7 cells. The results can be helpful in understanding the anticancer mechanism of p- coumaric acid and using it was suggested as an alternative or complementary drug in cancer chemotherapy.

Full-Text [PDF 497 kb]   (4141 Downloads)    
Type of Study: Original article | Subject: Oncology
Received: 2016/01/13 | Accepted: 2016/05/1 | Published: 2016/08/8

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