Comparison of sperm PLCζ protein and DNA damage between fertile and infertile men

tavalaee, Marziyeh; Arbabian, Maryam; Nasr- Esfahani, Mohammad Hossein

Volume 25, Issue 7 (oct 2017) JSSU 2017, 25(7): 585-594 | Back to browse issues page

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tavalaee M, Arbabian M, Nasr- Esfahani M H. Comparison of sperm PLCζ protein and DNA damage between fertile and infertile men . JSSU 2017; 25 (7) :585-594
URL: http://jssu.ssu.ac.ir/article-1-4096-en.html

Comparison of sperm PLCζ protein and DNA damage between fertile and infertile men

Marziyeh Tavalaee ^*

, Maryam Arbabian

, Mohammad Hossein Nasr- Esfahani

Abstract: (3964 Views)

Introduction: Sperm-specific phospholipase C zeta (PLCζ) protein is expressed during spermatogenesis and plays a main role during oocyte activation. In this study, the expression of sperm PLCζ protein and DNA damage in fertile and infertile men was assessed.
Methods: In this case-control study, semen samples were collected from 15 fertile and 10 infertile men candidated for intra-cytoplasmic sperm injection (ICSI). Sperm parameters, the expression of PLCζ protein, and DNA damage were assessed by the World Health Organization (2010) protocol, Western Blot, and TUNEL assay, respectively.
Results: The quality of sperm parameters were significantly lower in the infertile men compared with the fertile men. In addition, the expression of PLCζ protein was significantly lower, and percentage of sperm DNA damage were significantly higher in infertile men than fertile men.
Conclusion: Our results clearly showed that low or absence expression of PLCζ and sperm DNA damage could be considered as factors involved in failed fertilization in these infertile men. Therefore, the evaluation of some tests such as PLCζ protein and chromatin tests to assess fertilization potential of a semen sample for infertile men are recommended.

Keywords: PLCζ, ICSI, DNA damage, Sperm parameters

Full-Text [PDF 765 kb] (1978 Downloads)

Type of Study: Original article | Subject: Physiology
Received: 2017/02/4 | Accepted: 2017/05/20 | Published: 2017/11/25

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