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Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016
Mohsen Mirzaee, ,
Volume 24, Issue 11 (2-2017)
Abstract

Introduction: Urinary tract infection (UTI) caused by E coli is one of the most common diseases in community. Colonization of E. coli and its attachment to the uroepithelium are mediated by adhesions such as P, Fim and S fimbriae. The present study was aimed to evaluate the prevalence of fimbrial virulence genes in Escherichia coli (E. coli) isolates from the patients with urinary tract infection in Tehran, Boroujerd and Sanandaj cities, Iran.

Methods: In this descriptive cross sectional study, 150 clinical isolates of uropathogenic E.coli were collected from the patients with urinary tract infection. All bacterial isolates were identified by standard biochemical laboratory methods; the fim, pap and sfa genes were detected using the PCR and Multiplex-PCR methods.

Results: One hundred forty-five (96.66%) isolates were positive for fim gene, one hundred forty (93.33%) isolates were positive for pap gene and seven (4.66) isolates were positive for sfa gene. Whole of the isolates were possessed at least one of the three virulence genes. Six (4%) isolates were positive for all genes.

Conclusion: The findings of this study showed the high frequency of fim and P fimbriae among uropathogenic E.coli isolates from the patients with urinary tract infection. Because of the higher prevalence of UTI in the presence of these genes, detection of the genes in urine samples may help in more suspicious and rapid management of UTI.


Frequency of fimH, papC, and aer in E. coli Strains Isolated from Patients with Urinary Tract Infection, and their Antibiotic Resistance Pattern
Zahra Abdolahzadeh, Akram Astani, Ahmad Mosadegh, Saeede Sadat Hosseini Mohammad Abadi, Mahmoud Vakili,
Volume 29, Issue 3 (5-2021)
Abstract
Introduction: E. coli is the predominant causes of urinary tract infection. Several virulence factors for bacterial infections in the urinary tract are required. In this study, we have considered several virulence factors in strains isolated from the patients with UTI in Yazd.
Methods: In this cross-sectional study in 2015-2016, 146 strains isolated were collected from the patients with urinary tract infection. After confirmation by phenotypic and genotype methods, frequency of gene fimH, pap C, aer was evaluated using specific primers by PCR method. The pattern of antibiotic resistance of isolates was determined by disk diffusion method.
Results: In the present study, the prevalence of virulence genes was fimH (87%), aer (85.6%) and papC (9.6%). Among 146 E. coli isolates, resistance rate for various antibiotics were as follows: 57.2% to Cotrimoxazole, 54.8% to Nalidixic acid, 45.9% to Cefazolin, 40.4% to Cefixime, 42.5% to Cefalotin, 41.8% to Cefalexin, 31.5% to Norfloxacin, 30.1% to Ofloxacin, 28.3% to Ciprofloxacin, 24% to Gentamicin, 19.9% to Amikacin, 1.4% to Nitrofurantoin, and 1.4% to  phosphomycin. The results showed that the most and less frequent resistant was seen in Cotrimoxazole and Nitrofurantoin, phosphomycin, respectively.
Conclusion: In this study, the frequency of fimH gene was higher than other genes. In addition, according to the pattern of antibiotic resistance nitrofurantoin and phosphomycin are suitable medicines for the treatment of patients in our region.

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