Journal of

---

Introduction:Toxoplasma gondii is a ubiquitous obligate intracellular parasite with a relatively broad host range infecting both mammals and birds. Toxoplasma proteins are strong antigens that can begin strong immune reactions, among which Rhoptry protein 1 (ROP1) can be named discharging from rhoptry cell-organ. ROP1 is regarded as a competitor for recombinant vaccines against toxoplasmosis. Therefore, the main objective of the current study was to evaluate the cloning and expression of ROP1 Toxoplasma gondii in a cloning vector as well as to create this recombinant antigen in order to be applied for later uses. Methods:Genomic DNA of Toxoplasma gondii was removed and reproduced by PCR, then the PCR product was cloned into the EcoR1 and BamH1 sites of cloning vector, pUET1, and transformed into Escherichia coli BL21 plysS strain. Moreover, pcROP1 was sub-cloned into the HindIII and EcoRI sites of the pcDNA3 in order to produce recombining eukaryotic declaration vector. The cloned ROP1 was verified by PCR, limitation enzymes (HindIII and BglΙ) digestion and nucleotide sequencing. Then, this recombinant antigen was covered applying IgM and ELISAIgG. Results:The study results demonstrated that a fragment of 757 bp was separated. In addition, nucleotide sequence analysis of the ROP1 cloned in pUET1vector revealed high homology (96%) with RH strain Gene Bank Accession (No. M71274). Conclusion:The recombinant ROP1 antigen in an IgM Rec-ELISA test can be replaced with the tachyzoite antigen in IgG and IgM serologic tests.

---

Introduction: This note addresses the scientific study of behavioral changes caused by Toxoplasma. About a third of the world's population is infected with the chronic form of the parasite, which is usually asymptomatic and causes severe symptoms only in the presence of the parasite in the eye and nervous system. The study of behavioral changes caused by this parasite has begun since the 1980s and continues to this day. According to the results of these studies, infected men and women exhibit behavioral and personality changes. The mechanism of these changes is still unclear, but there are hypotheses about it that are under evaluation.
 

---

Introduction: More than 500 million people throughout the world are infected with Toxoplasma gondii. On the other hand, migraine is known as the most common pain syndrome. The aim of this study was to detect anti-Toxoplasma gondii IgG in the serum of individuals with and without migraine.
Methods: In this descriptive-analytical study, 50 person (7 male & 43 female, in the age range of 20-60 years) with history and symptoms of migraine (case group), and 50 individuals (7 male & 43 female, in the age range of 20-60 years) without migraine (control group) were selected randomly. Blood samples (5 ml) were collected from all the selected people, and the serum level of anti-Toxoplasma gondii IgG were determined using Enzyme-Linked Immunosorbent Assay (ELISA) technique. In this test, 10 IU/ml of anti-Toxoplasma gondii IgG was considered as the minimum titer. For statistical analysis SPSS Inc, Chicago, IL; Version 18 software and -Chi-square and t-tests were used.
Results: 38% of the patients with migraine and 32% of the control group had anti-Toxoplasma gondii IgG above 10 IU/ml. The mean amount of anti-T. gondii IgG in the serums of case group was 173.42 IU/ml, while it was 68.25 IU/ml in the control group, the mean amount of Toxo-IgG in migraine positive group was 2.5 time higher than the amount in the control group (p<0.05).
Conclusion: According to the results of this study it is concluded that the serum level of anti-Toxoplasma gondii IgG in migraine positive persons is significantly higher than the migraine negative individuals. Therefore, it is recommended that the patients disordered with migraine be tested for chronic toxoplasmosis..