Showing 2 results for Pcr-Rflp.
Kh Onsory, N Vahabi Barzi, Z Haji Mehdi Nouri, M Mousavi,
Volume 23, Issue 7 (10-2015)
Abstract
Background: A series of biochemical reactions are involved in the endogenous production of estrogens. Their final and rate-limiting step is catalyzed by aromatase belonging to the class XIX of cytochrome P450. CYP19 is a key enzyme for estrogen synthesis in males. It catalyzes the irreversible conversion of androstenedione and testosterone to estrone and estradiol-17&beta, respectively. Aromatase P450 is present in the endoplasmic reticulum of estrogen-reproducing cells in which it is expressed. The effects of the resulting estrogens are mediated through the estrogen receptor. One of the most important polymorphism, is a C to T variation in exon 7 resulting in an Arg264Cys amino acid exchange, has been shown to be very common in Asia. The purpose of this study was to determine the association of CYP19 gene polymorphism with the prostate cancer risk among the studied population. Methods: PCR-RFLP analysis of CYP19 gene was on 100 prostate cancer patients and an equal number of matching controls. The data was analyzed using the computer software SPSS for windows (version 19). Results: The frequency of CT genotype was higher in patients (37%) as compared to controls (21.2%) and this incidence was statistically significant (OR, 2.10 95 % CI, 1.02-4.34 P=0.044). Stratification of patients according to the risk factors, resulted in a slightly improved OR in individuals carrying CT compared to CC genotype (OR, 2.35 95% CI, 1.11-4.96 P=0.024). The TT genotype was not significantly associated with prostate cancer risk (OR, 0.63 95% CI, 0.16-2.50 P=0.519). Conclusion: It seems that CT genotype is more associated with cancer prostate compare with other genotypes. It appears to be an increased risk of prostate cancer associated with the Arg264Cys substitution in the CYP19 gene.
Mahboobeh Ahmadi, Seyed Mohammad Moshtaghioun, Ali Falahati,
Volume 26, Issue 7 (10-2018)
Abstract
Introdution: Recent studies identified the 15q14/15q25 chromosomal region as a susceptibility locus to lung cancer. This region includes CHRNA3, CHRNA5 and CHRNB4, genes, which coding for nicotinic acetylcholine receptor subunits in lungs and brain. The relationship between several polymorphisms in this locus and lung cancer risk has been widely investigated. The aim of this study was to survey the association of CHRNA3 rs1051730 C>T polymorphism with the risk of lung cancer in Iranian patients.
Methods: In this case-control study 96 lung cancer cases in comparison to 100 healthy controls were investigated for the frequency of rs1051730 C>T polymorphism using PCR-RFLP and DNA sequencing methods. The association between the genotypes and allele frequencies with lung cancer was analyzed using chi square and binary logistic regression by SPSS version 22 software.
Results: Allele and genotype frequencies of this SNP was not significantly different between case and control groups. The results of this study indicated no significant association between this polymorphism and lung cancer risk in the population studied (OR = 0.566, 95% CI = 0.239-1.342, P= 0.194).
Conclusion: Our results showed no significant association between polymorphism rs1051730 C>T and lung cancer risk in the population studied. However, according to the different results obtained from the populations of other studies, further studies are recommended along with an increase in the sample size.