Showing 7 results for Mesenchymal Stem Cells
Mohseni Kouchesfahani , Nabiuni, Delaviz, Bahrebar , Gheibi, Eslami,
Volume 19, Issue 5 (12-2011)
Abstract
Introduction: In this study the effect of vitreous humor on the mesenchymal stem cells (MSCs) derived from bone marrow to lens fiber-like cells was investigated.
Methods: In this experimental study bone marrow cells were collected by flushing femurs and tibias in NMRI mice. Immunocytochemistry by Oct4 antibody was used to confirm that the cells are stem cells. The mesenchymal character of these cells was proven by their adherence and by such flocytometery markers as CD44, CD31. In experimental groups, MSCs were cultured with DMEM and bovine vitreous body for induction. The supplemented medium was changed every two days. The expression of α –crystalline, as the marker of lens differentiation, was detected by immunocytochemistry.
Results: During the primary culture, the cell population was heterogeneous where varying morphologies such as flat, spindle-shaped, and polygonal were observed. In the subsequent passages, the number of the spindle-shaped cells appeared to increase, so that in passage 3 the majority of the cells seemed morphologically to be spindle-shaped.
Immunocytochemistery study confirmed the presence of stem cells using Oct4 antibody. The flowcytometric analysis of surface markers revealed that mesenchymal bone marrow stem cells express CD44 and CD31 to a low level. Morphological studies showed that most cells in experimental groups were locally longer and more aligned in parallel compared to control group cells. α –crystalline expression proved the formation of lens fiber-like cells.
Conclusion: According to the findings of this study, it can be concluded that MSCs derived from mouse bone marrow differentiate into lens fiber like cells by treating them with vitreous humor.
Mrs S Hamounnavard, Dr N Delirezh, Dr N Afzal Ahangaran,
Volume 22, Issue 4 (10-2014)
Abstract
Introduction: Mesenchymal stem cells have immunomodulatory properties and own extensive potentials to proliferate and differentiate into different cell lineages. Thus, this study was conducted to investigate the effect of supernatant of rat MSCs on the neutrophils viability.
Methods: MSCs was isolated from femoral and tibial bone marrow of rat (6-8 weeks) and was cultured in DMEM. After maturation of MSCs, its supernatant was incubated with neutrophils isolated from peripheral blood of rat at 37 ° C for 1 h. Neutrophil survival was measured at 6 and 24 h incubation with supernatant of MSCs by flow cytometric analysis using An/PI. Data were analyzed by one-way ANOVA followed by Tukey test (P˂0.05).
Results: 6-hour incubation of neutrophils with supernatant of MSCs significantly increased the healthy cells percentage and significantly decreased the amount of necrosis (P˂0.05), but no significant decrease was observed in regard with apoptosis compared to the controls (P˃0.05). The 24-hour incubation of neutrophils with cell supernatant significantly increased the percentage of healthy cells and apoptosis was significantly reduced compared to the control group (P˂0.05). Moreover, a reduction in cell necrosis was not significant in the treated groups compared to the control (P˃0.05).
Conclusions: In addition to the clinical importance of MSCs, their biological aspects are of great potential for cell therapy, such as self-renewal, proliferation and immune modulatory effects.
Mh Porghara, Ar Talebi, H Nahangi, M Anvari, F Sadeghian, A Yonesnia, M Mohammadzadeh,
Volume 23, Issue 5 (8-2015)
Abstract
Introduction: Scaffold has therole of the extracellular matrix in regulating cell survival and it is an important component in tissue engineering techniques. One of the scaffolds that was used in this field, was PLLA (poly L-lactic acid) which was usually prepared by electro spinning method. The purpose of this study was to evaluate the biocompatibility of nano-porous PLLA membrane on proliferation of mesenchymal stem cells.
Method: PLLA nano-porous membranes were prepared by phase separation process. To identify the type of polymer used in its construction, FTIR analysis was performed. Then, dental pulp mesenchymal cells were cultured on a nano-membrane and nano-membrane-free environments ,simultaneously. After attachment of the cells on the bottom of the plate, the biocompatibility and cell proliferation was assessed in a two-week period using MTT test on the fifth, seventh and ninth days of cell culture. The ultra-structure and adhesion of mesenchymal cells on the membrane were evaluated using scaning electron microscopy(SEM).
Result: Nano Membrane potential had good biocompatibility and the cells had penetrated into the surface membranes properly. The viability of mesenchymal cells cultured on nano-membranes were significantly higher than the control group. The highest effect of nano-membrane on cell viability was seen on the ninth day and the lowest effect was seen on the fifth day.
Conclusion: Due to the biodegradable and non-toxic properties of nano PLLA membrane, it could increase the adhesion and proliferation of mesenchymal stem cells and these effects will exacerbated over time.
Malek Soleimani Mehranjani, Fatemeh Darbandi, Atena Sadat Azimi,
Volume 26, Issue 5 (8-2018)
Abstract
Introdution: Bisphenol A (BPA) disturbs the morphology, viability and differentiation of rat bone marrow mesenchymal stem cells (rMSCs) to osteoblast via the production of free radicals. Vitamin C (vit-C) is a potent antioxidant and protects cells against oxidative stress. The aim of this study was to investigate the effect of co-treatment of BPA and vitamin C as an antioxidant on the viability and osteogenic differentiation of rMSCs.
Methods: In this experimental study, rMSCs were divided into 4 groups; control, BPA (200nM), BPA (200nM) + vit-C (300µM) as well as vit- C (300µM) and treated for 21 days in the osteogenic media. Then, cell viability, osteogenic differentiation, morphological changes and DNA breakage in different groups of cells were evaluated. Data were analyzed using one-way ANOVA and Tukey‘s test and the means were considered significantly different at P<0.05.
Results: A significant reduction in the cell viability, bone matrix mineralization, alkalin phosphatase activity and intracellular calcium concentration (P<0.001) as well as a considerable increase in DNA breakage was seen in the BPA group compared to the control. The above parameters were compensated in the BPA + Vit-C group to the control level.
Conclusion: The results of this investigation showed that Vit-C can compensate the adverse effects of BPA on the viability and osteogenic differentiation of rMSCs.
Seyed Mehdi Hoseini, Maryam Moghaddam-Matin, Ahmad Reza Bahrami, Fateme Montazeri, Seyed Mehdi Kalantar,
Volume 28, Issue 12 (2-2021)
Abstract
Introduction: Amniotic fluid contains a mixture of different cell types sloughed from the fetal skin, respiratory, alimentary and urogenital tracts, as well as the amnion membrane. As amniotic fluid develops prior to the process of gastrulation, many cells found in its heterogeneous population do not undergo lineage specialization. Therefore, amniotic fluid-derived mesenchymal stem cells (AF-MSCs) may correspond to a new class of stem cells with properties of intermediate plasticity between pluripotent and adult stem cell types. Compared to mesenchymal stem cells (MSCs) from other sources, such as bone marrow, AF-MSCs have better properties for clinical applications, such as differentiation into the cells of three germ layers, high clonal capacity, ability to form embryoid bodies, expression of pluripotent markers, high self-renewal capacity (over 250 population doublings) with normal karyotype at late passages, long telomere length due to continued telomerase activity, specially non-tumorigenicity, low immunogenicity, anti-inflammatory and immunomodulatory properties.
Conclusion: Such features have nominated AF-MSC for a range of clinical applications, including in regenerative medicine. In several studies, these cells have been used to regenerate nerve, lung, and heart tissues. Overall, AF-MSCs are expected to be an ideal source of stem cells for future regenerative medicine and tissue engineering.
Mansour Esmailidehaj, Hassan Esmaeili, Elham Chavoushi, Mohammad Ebrahim Rezvani, Hossein Azizian,
Volume 30, Issue 4 (7-2022)
Abstract
Introduction: Numerous studies have shown that enriched plasma protects myocardial cells against ischemia. The aim of this study was to evaluate the effect of rat enriched plasma by preconditioning, ischemia, and reperfusion on the differentiation of bone marrow mesenchymal stem cells (BMMSC) into cardiomyocytes.
Methods: In this experimental and laboratory study, BMMSCs were extracted from the femur bone of male Wistar rats weighting 250-300 g. BMMSCs of the third passage were divided into six groups according to the type of plasma received: 1) control, 2) azacytidine, 3) azacytidine and non-ischemic plasma recipient, 4) azacytidine and preconditioned plasma recipient , 5) azacytidine and ischemic plasma recipient 6) azacytidine and plasma reperfusion recipient groups. In all groups except group 1, to initiate cardiomyocyte differentiation, BMMSCs were exposed to azacytidine (10 μM) for 24 hours. Plasma (25 μg / ml) was added to the culture medium each time the culture medium was changed. After 21 days, the expression level of myocardial βMHC, desmin, and troponin I genes was assessed using qRT-PCR.
Results: The expression of myocardial βMHC, desmin, and troponin I genes was significantly increased in all groups received azacytidine (groups 2 to 6) (P<0.01). There was no significant difference in the expression of myocardial βMHC, desmin, and troponin I genes, the doubling time, and migration between the plasma receiving groups (groups 3 to 6) compared to the group 2 (P>0.05).
Conclusion: The findings of present study showed that the plasma collected during ischemia, ischemia-reperfusion, and ischemia preconditioning had no effect on the differentiation of BMMSCs into cardiomyocytes in the presence of azacytidine.
Samira Mozaffari Khosravi, Saman Seyedabadi, Seyed Hossein Shahcheraghi, Marzieh Lotfi,
Volume 32, Issue 2 (5-2024)
Abstract
Introduction: Women of reproductive age may encounter challenges related to infertility or miscarriage, due to conditions such as premature ovarian failure (POF). POF, affecting approximately one percent of women under the age of 40, can lead to infertility. Current data have suggested that utilizing stem cell therapy was the most effective approach for treating POF compared to alternative options. Among the various stem cell types, mesenchymal stem cells derived from the umbilical cord (HUC-MSCs) was a promising choice for treatment due to its features such as relatively low immunogenicity, multipotent, multiple origins, cost-effectiveness, ease of production, and high efficiency.
Conclusion: One of the ways to treat POF is the use of mesenchymal stem cells derived from the umbilical cord, these cells have an extraordinary capacity for repair and regeneration, which helps them in repairing depleted ovaries. The results of this review article indicate that the use of HUC-MSCs can be considered as a potential treatment method in these patients.
