Search published articles


Showing 4 results for Male Infertility

H Pashaiefar, Mh Sheikhha, M Kalantar , T Jahaninejad, Ma Zaimy ,
Volume 21, Issue 1 (4-2013)
Abstract

Introduction: Infertility is described as the inability to get pregnant after one year of unprotected intercourse. About half of infertility cases are because of male factors. Idiopathic azoospermia or severe oligozoospermia caused by genetic alterations is a significant part of male infertility. A key step of spermatogenesis is crossover events during meiotic reciprocal recombination. MLH3 protein has a crucial role in meiotic recombination and in spermatogenesis. We evaluated this function of MLH3 protein by examining the contribution of functional polymorphism in MLH3 (C2531T) to the risk of male infertility. Methods: We studied this polymorphism in 110 infertile male with idiopathic azoospermia or severe oligozoospermia, and 110 fertile men with normozoospermia as a control group. MLH3 C2531T polymorphism was analyzed using the tetra-amplification refractory mutation system-PCR (4P-ARMS-PCR) method. Results: Genotypes CC, CT and TT of the MLH3 gene presented frequencies of 13.6%,59.1% and 27.3%, respectively, in the men with idiopathic azoospermia or severe oligozoospermia and 37.3%,53.6% and 9.1% in the control group (p<0.001). Conclusion: The data suggest that the MLH3 C2531T polymorphism can be associated with risk of male infertility. The research data showed that presence of the polymorphic allele T leads to an increased risk of 2.35 times (OR =2.35, 95% CI =1.57-3.51 p<0.001) to develop infertility in relation to the normal control group. Therefore, the MLH3 gene polymorphism may be genetic determinant for defective spermatogenesis in the humans.
F Tajbakhsh, F Mashayekhi, A Hamidi Madani, Mh Bahadori,
Volume 23, Issue 1 (4-2015)
Abstract

Introduction: Infertility is defined as the inability of the couples to achieve pregnancy after 12 months of unprotected intercourse. P-glycoprotein (P-gp) also known as multidrug resistance protein 1 (MDR1) is an important protein of the cell membrane that pumps many foreign substances out of cells and has a protective role in sensitive tissues such as testis. MDR1 gene is located on q21.1 of chromosome 7. Hence, this study aimed to assess the association of MDR1 polymorphism and idiopathic male infertility. Methods: In this study (case- control), DNA was extracted from blood leukocytes of 136 male patients with idiopathic infertility as well as 130 healthy men. Genotypes were determined by using PCR-RFLP technique and EcoO109I enzyme. Results: The genotypes frequencies of CC, CT and TT in patient group were 19.12%, 39.70% and 41.18%, respectively, and the genotype frequencies of CC, CT and TT in control group were 12.30%, 61.54% and 26.16%, respectively. Conclusion: The study findings revealed that a significant association was found between MDR1 polymorphism and idiopathic infertility (P= 0.001). Therefore, the results suggest that CT heterozygous genotype has a protective effect on male fertility (P= 0.01, OR= 0.41 95%CI: 0.23- 0.84). However, to achieve more accurate results, it is necessary to examine a larger target population.
Atefe Yadollahykhaless, Naser Kalhor, Golnaz Atri Roozbahani,
Volume 25, Issue 8 (11-2017)
Abstract

Introduction: Spermatogenesis is a strictly regulated process in sperm production that is needed for
sperm production transcriptional and posttranscriptional regulations. The cytoplasmic polyadenylation element binding (CPEB) protein regulates cytoplasmic polyadenylation of mRNAs in oogenesis and spermatogenesis. The purpose of the present studywas examining the association between rs2303846, which is located at 3'UTR of CPEB gene, and Iranian male infertility.
Methods: In this case–control analysis, 140 blood samples were collected (70 fertile men and 70 infertile men). rs2303846 genotypes were determined by PCR-RFLP method and the results were confirmed by sequencing. The differences in genotype distributions between cases and fertile controls were examined using Chi-squared analysis and SNPSTAT software. Corelation between this SNP and miRNAs was examined.
Results: TT genotype was observed in 5 infertile men among 70 case samples, while all control samples showed CC genotype and there was a significant association in this difference (P= 0.023). This SNP exist in seed region of three miRNAs (hsa-miR-143-5p, hsa-miR- 6511b-5p, hsa-miR-3944-5p) and T allele causes seed destruction. In addition, T allele leads to the loose binding of all the selected miRNAs.
Conclusion: Our results indicate that rs2303846, which is located on CPEB1 is associated with the risk of infertility in Iranian population. Our bioinformatics analysis showed that changing the T allele of rs2303846, instead of C allele could loose miRNA binding to their target genes in our selected miRNAs. Consequently, the gene is stable and its expression continues.
Mahsa Nasr Esfahani, Seyed Mehdi Kalantar, Fatemeh Montazri, Mahya Rajabi, Fatemeh Daneshmand ,
Volume 29, Issue 11 (2-2022)
Abstract

Introduction: Not being to get pregnant, after one year of unprotected sex is called infertility. In the post, infertility was mainly a female problem, but the role of male factors in infertility has denotation, although a greater percentage of this infertility is related to the deficiencies of semen.
Methods: This case-control study was performed on sperm samples from 30 infertile oligoteratozoospermia (OT) patients as case group and 30 fertile and normosperm as controls, all the patients and controls between the ages of 20-49 years, who were referred to the Yazd Infertility Center for the treatment of infertility, were used to check the chromosomes 18, X and Y using the FISH technique. The results were considered using SPSS version 16 software and statistical tests T-TEST and Chi-Square Tests for statistical analysis and Pearson R correlation coefficient to measure the relationship between variables and statistical level P <0.05.
Results: Disorders were observed in infertile oligoteratozoospermia men who were significantly related to the control group and between the chromosomal abnormalities, sperm counts and morphology )P<0/01) and there was also significant difference in correlation between chromosomal abnormalities and duration of infertility  (P<0/01). In addition, there was no correlation between chromosomal abnormalities and age, but the rate of chromosomal abnormalities in the age group of 40-49 years increased to 50%, which has the highest rate among age groups and definitely needs to be examined in a larger statistical population.
Conclusion: This finding suggest that patients with OT may be at an increased risk of producing aneuploid offspring. Considering the chromosomal aneuploidy is recommended by cytogenetic molecular techniques.
 
 


Page 1 from 1     

© 2025 CC BY-NC 4.0 | SSU_Journals

Designed & Developed by : Yektaweb