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Showing 2 results for Lipid Peroxidation

N Farhangi, F Nazem, F Zehsaz,
Volume 24, Issue 10 (1-2017)
Abstract

Introduction: Oxidative stress can promote the development of complications of diabetes. Moderate exercise improves cardiac antioxidant status in diabetic animals. The current study aimed to investigate the effect of 8 week endurance exercise training on some heart antioxidant enzyme activities and lipid peroxidation of heart tissue in streptozotocin-induced diabetic rats.

Methods: To this end, 30 male Wistar rats were randomly divided into 3 groups: the healthy control group (C), diabetic control group (DC) and trained diabetic group (TD). Animals in TD group were exercised on a treadmill 4 days a week for 8 weeks. After 8 weeks, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities and malondialdehyde (MDA) levels were measured in heart tissues. The data were analyzed using one-way ANOVA (p<0.05).

Results: The CAT and GPx activities of the heart tissue in DC group were found to be significantly higher (p=0.000) in compared with C group, whereas SOD activity was not found significantly different among the experimental groups. Also, heart GPx activity in TD group was significantly lower (p=0.000) than that of DC group, while CAT activity was not affected by endurance training. After 8-week endurance exercise (TD group), the MDA levels of heart tissue were significantly higher (respectively p<0.017 and p=0.000) than C and DC groups.

Conclusion: The results of the present study have demonstrated that due to decreased GPx activities and MDA levels, endurance exercise may make the heart tissue more susceptible to oxidative stress.


Fatemeh Asadian, Mohammad Ali Takhshid,
Volume 30, Issue 7 (10-2022)
Abstract

Introduction: Antioxidant defense system, including enzymatic and non-enzymatic antioxidants, protects cells from oxidative damage by neutralizing oxidant compounds. Increasing the production of oxidant species and decrease activity of antioxidant system causes oxidative stress. The role of oxidative stress in the pathogenesis of human diseases and strengthening of human antioxidant defense system in preventing and ameliorating of diseases have been shown in numerous studies. Therefore, identifying reliable oxidative stress markers for evaluating the beneficial effects of antioxidants in human diseases has been the focus of many studies over the past decades. Measuring free radicals and active species by flow cytometry, determining the total antioxidant capacity of body fluids, measuring the oxidation products of macromolecules, determining the activity of antioxidant enzymes, and changing the expression of genes related to the antioxidant system are among these methods. This review discussed the major advantages and limitations of these methods.
Conclusion: Due to limitations and potential confounding factors of the current markers of oxidative stress, an appropriate experimental protocol should be used for each study and caution should be taken in the interpretation of results. Furthermore, more than one method should be used to overcome the limitations of each marker.
 


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