Mohseni Kouchesfahani , Nabiuni, Delaviz, Bahrebar , Gheibi, Eslami,
Volume 19, Issue 5 (12-2011)
Abstract
Introduction: In this study the effect of vitreous humor on the mesenchymal stem cells (MSCs) derived from bone marrow to lens fiber-like cells was investigated.
Methods: In this experimental study bone marrow cells were collected by flushing femurs and tibias in NMRI mice. Immunocytochemistry by Oct4 antibody was used to confirm that the cells are stem cells. The mesenchymal character of these cells was proven by their adherence and by such flocytometery markers as CD44, CD31. In experimental groups, MSCs were cultured with DMEM and bovine vitreous body for induction. The supplemented medium was changed every two days. The expression of α –crystalline, as the marker of lens differentiation, was detected by immunocytochemistry.
Results: During the primary culture, the cell population was heterogeneous where varying morphologies such as flat, spindle-shaped, and polygonal were observed. In the subsequent passages, the number of the spindle-shaped cells appeared to increase, so that in passage 3 the majority of the cells seemed morphologically to be spindle-shaped.
Immunocytochemistery study confirmed the presence of stem cells using Oct4 antibody. The flowcytometric analysis of surface markers revealed that mesenchymal bone marrow stem cells express CD44 and CD31 to a low level. Morphological studies showed that most cells in experimental groups were locally longer and more aligned in parallel compared to control group cells. α –crystalline expression proved the formation of lens fiber-like cells.
Conclusion: According to the findings of this study, it can be concluded that MSCs derived from mouse bone marrow differentiate into lens fiber like cells by treating them with vitreous humor.
Seyed Hosein Tabatabaei, Laleh Maleki, Fatemeh Sohrabi,
Volume 26, Issue 4 (7-2018)
Abstract
Introdution: Angiogenesis is important for tumor growth and metastasis. It is determined by microvessel density (MVD). The goal of this study was to evaluate microvessel density in the salivary glands mucoepidermoid carcinoma (MEC) and its association with histological grading.
Methods: In this cross-sectional study, 17 mucoepidermoid carcinoma paraffin blocks were extracted. CD34 immunohistochemically staining was done for evaluation of vascular density. Microvessel density was measured by vessel counting at ×400 magnification, in vascular areas with the highest concentration. The obtained results were analysed using ANOVA method and post hoc test of Scheffe.
Results: The mean of microvessel density in low, intermediate and high grades were 31.66, 47.66 and 47.8, respectively. Significant difference was observed between the mean of microvessel density and histological grading of mucoepidermoid carcinoma (P =0.024). The mean of microvessel density in the high and intermediate grades tumors were significantly more than low grade (p =0.039 and p =0.025, respectively), but there was no significant difference in the mean of microvessel density between the high and intermediate histological grades )p=1.000).
Conclusion: According to the results, there is a significant association between angiogenesis and histological grades of mucoepidermoid carcinoma and also more angiogenesis is observed in tumors with higher malignant grade.
