Showing 6 results for Gastric Cancer
Sh Keyhanian, N Farhadifar, Z Fotoukian, M Pouya, M Saravi,
Volume 20, Issue 1 (5-2012)
Abstract
Introduction: Gastric cancer is considered as one of the most prevalent cancers, such that in our country it is on the top of prevalent cancers. Because of the high prevalence of this cancer in north of Iran, in spite of its incidence decrease in many regions of the world, we decided to determine the epidemiological and malignancy properties of gastric cancer in this region
Methods: This research is a descriptive study (based on existing data). The study population consisted of gastric cancer patients referred to oncology clinic at Ramsar Imam Sajjad hospital during 2002- 2009. Necessary information was extracted from their medical files and pathology reports, and statistically analyzed.
Results: In this Study, 144 patients were assessed (103 males and 47 females). In 95.8% of patients, tumor was adenocarcinoma. In 45.1% tumor was located in antrum. In 48.6% tumor was poorly differentiated. 72.9% of subjects had positive lymphatic involvement. There was metastatic involvement in 31.9% of patients, and the most common organ for metastasis was liver. 46.5% of Patients were in stage 4 at the time of referring to hospital and in 38.2% of cases tumor has been progressed to T2 Level.
Conclusion: By considering this fact that most referring patients (46.5%) were in stage 4 when were diagnosed and had poorly differentiated carcinoma, it is recommended that people should consider abdominal discomfort as a serious problem and do necessary diagnostic methods especially in ages above 45 years with especial emphasis on early diagnosis of disease and reduction of death rate for at risk population.
M Hemati, N Eivazi, A Chakerzehi, A Moradi, J Mohiti Ardakani, A Mahmoodzade,
Volume 22, Issue 2 (6-2014)
Abstract
Introduction: Understanding the molecular mechanisms of metastasis is important for the design and effective use of novel therapeutic strategies in order to combat metastases. One class of molecules that has been implicated in metastasis is Rac1. Regarding complications of conventional therapies such as chemotherapy, current studies are evaluating natural compounds with anticancer Properties. Melittin is a natural compound derived from honey bee venom which has revealed amphipatic properties. Some studies have shown that melittin has an important role in reducing cancer cells metastasis by affecting Rac1 expression. Since the studies on melittin role in regard to Rac1 in gastric cancer cells are rare, the present study examined the effect of melittin on Rac1 protein expression in AGS gastric cancer cells.
Methods: AGS cells, after growth and reaching to 80% density, were exposed to 0.2, 0.3, 0.4 μg/ml concentrations of melittin (test groups) for six hours. Then cells were collected, lysed with lysis buffer and Rac1 protein was identified by Western blotting technique. Data were normalized with the β-Actin internal control and calculated as the relative percentage. The study data were analyzed using SPSS statistical software via One-Way Anova test.
Results: Comparison of Rac1 expression levels in AGS cancer cells showed that cells which were exposed to 6 hours of 0.2μg/ml (109±5.5), 0.3(105±3.6) melittin had higher Rac1 expression percentages compared to the control (100) and 0.4 (100±2). Rac1 expression percentages were not significant with a confidence interval (CI) of 95% ( P>0.05 ).
Conclusion: In this study the results demonstrated that melittin does not have any effect on Rac1 in gastric cancer cells (AGS).
Fatemeh Gohari-Ensaf , Zeinab Berangi , Mohamad Abbasi, Ghodratollah Roshanaei ,
Volume 27, Issue 12 (2-2020)
Abstract
Introduction: Gastric cancer is the fourth most common cancer and the second leading cause of death in the world. Despite the recent advances in controlling and treating the disease, the survival rate of this cancer is relatively low. Various factors can affect the survival of the patients with gastric cancer. The aim of this study was to determine the survival rates and the effective factors in the patients with gastric cancer.
Methods: The study population included all the patients diagnosed with gastric cancer in Hamadan Province who were referred to Hamadan Imam Khomeini Specialized Clinic between 2004 to 2017. Patients were followed up by periodical referrals and/or telephone contact. The survival rate of the patients was calculated using Kaplan-Meier method and effective survival factors with Cox proportional regression. Data were analyzed using SPSS 23 software at a significance level of 0.05.
Results: Out of the 350 patients with gastric cancer, 74.3% were male and 25.7% were female. One-year, three-year and five-year survival rates were 67%, 36% and 27%, respectively. The log -rank test showed that age, type of tumor, stage of disease, type of Surgery and metastasis of the disease were effective on the survival of patients. In Cox's multivariate analysis, the only age variables at the time of diagnosis and chemotherapy were survival variables. (P<0.05).
Conclusion: The results of this study showed that age variable is a strong factor in survival, so it is essential to diagnose the disease at the early age and early stages of the disease using a screening program.
Mahshad Hooshyar, Saeed Abedian-Kenari, Abbas Mohammadpour, Habibeh Mirmajidi, Majid Jafari-Sabet, Ramin Ataee,
Volume 29, Issue 6 (9-2021)
Abstract
Introduction: Gastric cancer is the fourth most common cancer and the second leading cause of cancer death worldwide. North of Iran is a high-risk area for gastric cancer. Nitric oxide (NO), mainly synthesized by inducible nitric oxide synthase (NOS2) in pathological conditions, plays an important role in cytotoxicity, inflammation and fibrosis. In this research we studied the effect of (rs1137933) T>C genotype on gastric cancer..
Methods: This analysis was performed on 93 patients with gastric cancer who were referred to endoscopy Tuba Clinic in 2015 and 93 healthy individuals as controls. DNA extracted from peripheral blood samples was applied in PCR-RFLP (Polymerase chain reaction- restriction fragment length polymorphism) analysis to determine (rs1137933) T>C genotype. The association of the (rs1137933) T>C genotype and gastric cancer risk were analyzed by MedCalc software and (X2) Chi-square and (OR) Odds Ratio exams.
Results: Frequency of TT, CT, and CC genotypes in cases was 12.61, 51.35 and 36% and 38.7, 34.4, and 26.8% in the control group. Significant association was found between (rs1137933) T>C genotype with gastric cancer chance, P<0.05, OR=2/04, 95% CI (1/37 to 3/03).
Conclusion: The results of the study show that the presence of CC+CT genotypes may increase the risk of gastric cancer. P < 0.0001, OR=4.37 (2.17 to 8.80).Therefore, investigating the (rs1137933) T>C single nucleotide polymorphism of NOS2 gene could be an appropriate molecular marker that could be used to determine individual sensitivity to gastric cancer and for designing cancer prevention programs.
Zahra Ahmadzadeh Chaleshtori, Ali Asghar Rastegari, Hashem Nayeri, Abbas Doosti,
Volume 30, Issue 12 (3-2023)
Abstract
Introduction: Helicobacter pylori (H. pylori) causes successive changes in the stomach wall, which starts with inflammation of the stomach mucosa and in some cases leads to stomach cancer. The leoA gene, by encoding GTPase, plays a vital role in the pathogenicity of this bacterium in the gastric mucosa. The secretory vesicles produced by the leoA gene release poison and stimulate the immune system in the host's body. The aim of the present study was to evaluate the gene vaccine pEGFP-C2-leoA on the expression changes of cytokines such as IL6, IL4, and interferon-gamma in inflammation caused by Helicobacter pylori infection in a mouse model.
Methods: In this interventional experimental study, recombinant plasmid (pEGFP-C2-leoA) was produced, propagated, and extracted through transformation into susceptible bacterial cells. Then, suitable concentrations of 1% chitosan nanoparticles solution were prepared for injection into the quadriceps muscle of BALB/c mice. Finally, the gene expression and changes of the mentioned cytokines were measured by Real-Time RT-PCR method, the obtained results were statistically analyzed by SPSS version 16 software; One way ANOVA test and subsequent LSD test, as well as independent t-test, were used to check the existence of correlation and the significance level of the data.
Results: After the injection of the vaccine into the quadriceps muscle of mice during the treatment period, cytokines IFNᵧ (<0.038) and IL6 (<0.049) showed a significant increase in expression. On the other hand, cytokine IL4 and the leoA gene also showed a significant decrease in expression (>0.042).
Conclusion: Based on the results, the leoA gene cloned in the expression vector pEGFP-C2 has the ability to express and produce the specific protein product of this gene in eukaryotic cells, and according to the results obtained in the animal model and the immune characteristic obtained in this research it is shown that the final construct pEGFP-C2-leoA has the necessary potential to investigate immunogenicity in a human model as a gene vaccine.
Abolfazl Hekmat-Shoar, Amirali Moodi Ghalibaf, Ali Shamshirian, Mohammadreza Abbaszadegan4, Bahram Memar, Mohammad Reza Motie,
Volume 31, Issue 4 (7-2023)
Abstract
Introduction: The present study was designed to investigate the relationship between COX-2 gene expression, clinicopathological symptoms, and prognosis of patients with gastric cancer.
Methods: The present cross-sectional study was designed to achieve the scientific aims among patients referred to the Imam Reza Hospital, Mashhad, Iran from 2008 to 2018. In this study, patients' demographic characteristics, clinical signs and symptoms, tumor findings, type of surgery, and COX-2 gene expression level (using Real Time PCR technique) were recorded. The data were analyzed by SPSS software (version 16), statistical tests, including Chi-square, Fisher's exact, Gamma coefficient, one-way ANOVA, or Kruskal-Wallis test at a significance level of 0.05.
Results: Out of 110 patients included in the study, 55 patients were evaluated according to the criteria. Among them 21 (38.2%) patients had decreased levels of gene expression, in 18 (32.7%) patients gene expression was in the normal range, and in 16 (29.1%) patients increased gene expression was seen. The average survival months of patients after surgery were 23.41 ± 23.07 months. No statistically significant relationship was found between increased COX-2 gene expression and clinical signs, age, sex, survival (by month), tumor progression, type of surgery, histological features, family history of cancer, and other cases (P> 0.05); However, considering the annual survival in the annual classification showed a significant relationship with the expression of COX-2 gene (P=0.028).
Conclusion: Despite no significant relationship between COX-2 gene expression and studied parameters in the patients with gastric cancer in this study, the existence of a significant relationship between gene expression and annual survival of patients seems so important.
