Showing 33 results for Breast Cancer
Zh Mirlashari, S Mohammad Ali Zadeh, M Azizzaheh Forozi,
Volume 12, Issue 3 (10-2004)
Abstract
Introduction: Secondry Prevention is Very important in breast cancer because when treatment is started,the breast mass will have smaler size. One of the most important way For Secondry Prevention of breast cancer is examination by Physician or health care provider.
Materials and Methods: In this Quasi experimental study that aimd to evaluating the effect of education about importance of CBE on attendance of kermanian teacher woman for examination, 501 subjects Participated. Schools were selected by multistage random sampling Method and divided in to experimental and control groups. An educational pamphelet were given to the experimental group and both groups were Informed about address and date of attendance. After two months an investigator-made questionnaire were responded by all of teachers.
Results: The results showed there was not significant differences in two groups according to those variables Could be probably effective on attendance, with exception of mariage status and education level . The attendance rate for CBE in experimental grorp (17.3%) was Significantly (p<0.001) higher than control (6%). The relationships between attendance rate with Personal characteristics and variables related to breast Cancer revealed the significant difference with history of breast cancer in Family only in experimental group (p<0.05) ,so that the attendance rate in teachers with positive history were higher.
Conclusion: Although the attendanee rate is not stisfactory in two groups but using the educational pamphelet that is simple, Not expensive and needless to direct contact with educator, attendance rate in experimental group was three time higher than control group
Z Ghazanfari , S Mohammad Alizadeh, F Azattalab,
Volume 14, Issue 2 (7-2006)
Abstract
Introduction: Today, breast cancer is one of the most common causes of mortality and morbidty and also the leading health concern of the world.
Methods: This research was a descriptive study entitled Knowledge, attitude and practice regarding prevention of breast cancer in working women of Chaloos city. The sample size of study included 612 women working in governmental centers. The instrument for data collection was a researcher –made questionnaire. For data analysis, central and coefficient index chi square ,t-test ,one way analysis of variation, Kruscal Wallis, Pearson,s correlation coefficient and Fisher test were used.
Results: The findings showed that the most of samples were in the 30-39 years age group,(39.7%) with an educational job(83/5%) and without history of breast cancer history in the family.(94/9%) Results also revealed that there was a weak positive relationship between knowledge and attitude (r=0.33) .The findings showed there was a significant statistical relation between knowledge and practice (p<0.05). Also, there was a significant statistical relation between knowledge level and breast self examination (BSE), time interval and also the time of performing BSE (p<0.0001) Similarly, the highest levels of knowledge were present in women with medical education and those with a bachelors or higher degree. The results showed that attitude scores were significantly different in various age, job and educational groups, (p<0.001, p<0.05,p<0.05) respectively
Conclusion: The results showed that most of the population under study had a positive attitude but didn’t have good knowledge or practice.
M Mir, P Mir, S Elmi, M Maleki,
Volume 14, Issue 4 (1-2007)
Abstract
Introduction: Breast Cancer is one of the most important fatal cancers in women. The mean age of breast cancer in Iran is 48.8 years which is very lower than other countries. Selenium can play an important role in reduction of cancer in several ways, for example selenium increases immunity response and protects cells from oxidation of free radicals and also decreases carcinogenic metabolites.
Breast cancer is one of the most important cancers in our country because its incidence is very high and the mean age of patients is very low. Different studies have shown the benefits of selenium in prevention of cancer and since many years selenium has been used as a dietary supplement in advanced countries. Several studies regarding relationship between selenium levels and breast cancer have been done in different countries. We therefore planned a study to evaluate serum selenium levels in breast cancer patients and compare them with a healthy control group.
Methods: We selected 45 patients younger than 48 years old and 33 patients older than 48 years old who had not yet received any therapy (surgery, chemotherapy, etc) for their cancer as a case group and 46 healthy people who were matched with the patients as a control group and included 23 persons younger and 23 persons older than 48 years old. From each participant, 5cc blood was derived and in several stages, serum selenium levels were evaluated using atomic absorption technology. Data about type of cancer, stage, grade, IHC and cigarette smoking were also collected.
Results: The mean Se level was 161.20 μg/l (SD=46.27 μg/l) in the patients and 189.13 μg/l (SD=48.75 μg/l) in the control group that was statistically significant (P<0.001).
The mean Se level in younger patients (< 48 years old) was 169.35 μg/l (SD=47.34 μg/l), while that in older patients (>48 years old) was 155.39 μg/l (SD=46.68 μg/l) that was lower than the control groups. Difference in serum selenium levels between patients and controls in the older group was significant (P=0.007), but in the younger group, it was not statistically significant (P=0.23).
Conclusion: Prescription of selenium as a dietary supplement may have a protective role against development of breast cancer, particularly in older ages and widespread studies need to be performed to prove this postulation.
Key Words: Breast cancer, Selenium
A Fazel, B Tirgari, N Mokhber, Mm Koushyar, H Esmaily,
Volume 16, Issue 3 (10-2008)
Abstract
Introduction: Breast cancer with 22.6% is the most common cancer in Iranian women and mastectomy is the treatment of choice in 81% of cases posted for surgery. Mastectomy can evoke feelings of mutilation, altered body image and decreased sexual attractiveness and function leading to mood disorders. In the last decades, final purpose of treatment strategies in breast cancer is creation of a life with good quality. In this regards, concern is rising about the impact of surgical treatments, especially mastectomy on patient`s quality of life. This study was conducted to determine the relationship of mastectomy with mood and quality of life in breast cancer patients.
Methods: This descriptive-analytic study was done in 2005.Case group included mastectomy patients referring to the oncology clinics (n=50).The comparison group consisted of mammography candidates referring to women clinics in Imam Reza and Ghaeem Hospitals. Data collected was based on non-probability and purposeful sampling. The information collected included: demographic and medical information, social support questionnaire, POMS survey for measurement of mood. Quality of life was assessed with FPQOLI.
Results: Pearson correlation coefficient showed a significant converse statistical relation between mood and quality of life score in mastectomy women (P<0.001). Independence T test showed that mood and quality of life in mastectomy group were lower than mammography candidate women (P=0.001, P<0.049). The general linear model test showed that group variable had the most effect on women`s mood (P<0.001). Also , mood was the most important factor that predicted level of quality of life in these women (P<0.001).
Conclusion: Lack of attention to psychological and spiritual status of the patients after mastectomy may provide conditions for unstable mood and can finally disrupt their quality of life. But, nurses can identify patients with psychological tensions and introduce them to consultancy centers to prevent mood disorders and promote their quality of life.
Ma Hosseinpour Feizi , S Moniri Javadhesari, E Babaei, V Montazeri , M Halimi ,
Volume 17, Issue 2 (7-2009)
Abstract
Introduction: Survivin is a new member of the Inhibitor Apotosis Protein family (IAP) which plays an important role in the regulation of both cell cycle and apoptosis. Its distinct expression in tumor cells as compared to normal adult cells introduces Survivin as the fourth transcriptom demonstrated in tumors. Breast cancer is the most common malignancy among women and scientist`s efforts to classify it has lead to various molecular subtypes and controversial results. Because of the high prevalence of these tumors and lack of suitable molecular markers for diagnosis and prognosis, there are ongoing efforts to find molecular markers which can distinguish nontumoral from tumor tissues. In this study we evaluate the potential usefulness of Survivin and its splice variants ΔEx3, 2b and 3b as molecular markers in breast cancer.
Methods: We studied 18 tumor and 17 non tumor adjacent tissues. Transcription levels were measured by Semiquantitative Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and normalized by ß2m as an internal control.
Results: 1)Survivin and its splice variants Δex3, 2b and 3b showed differentially higher expression levels in tumors than adjacent normal tissues. 2) The expression levels of Survivin, Survivin-ΔEx3 and Survivin-3b were significantly correlated with the type of tumors. 3) Survivin-2b was expressed in a few samples. 4) Survivin-3b was detected only in tumor samples. Also, our results showed that ΔEx3 variant can be introduced as a dominant expressed variant in breast cancer.
Conclusion: Our data indicated that the expression of Survivin, Survivin ∆Ex3 and especially, Survivin-3b were correlated with cancerous nature of tumors and Survivin-∆Ex3 was the most common expressed variant in breast carcinomas. These results besides confirming the potential usefulness of Survivin and its splice variants as molecular markers in breast cancer, demonstrated the role of the gene and its splice variants, especially 3b in these lesions that enables distinguishing normal from tumor tissues. Therefore, evaluation of the expression of Survivin and its splice variants might be used as markers to stratify breast cancer patients for more optimal treatment modalities or it could be a promising new target for therapy.
B Ghezelbash, Z Mohammad Hassan , F Ghaderi Pakdel , S Zare,
Volume 19, Issue 5 (12-2011)
Abstract
Introduction: The probiotic strains of Lactic Acid Bacillus (LAB) not only affect gastrointestinal tract microflora and stimulate local immune system of this tract but also modify and stimulate systemic immunity by influence on lymph nodes and spleen. Several studies have shown the anti-tumor effect of these kinds of bacteria. This study was designed to assess the probiotic effects of lactobacillus rhamnosus on cisplatin efficacy among Balb/c mice with breast cancer.
Methods: L. rhamnosus strain was inoculated in MRS agar and cultivated for 24 h at 37 °C. Female BalbC mice (n=20) with invasive ductal carcinoma transplantation were divided into four groups: Control, L. rhamnosus, cisplatin and cisplatin plus L. rhamnosus. Cisplatin (5 mg/kg, i.p.) was injected twice a week. Lr was administered daily by gastric intubation (3×10 8 CFU/day). The tumor size was measured every 3 days and mice were sacrificed 24 h after the last injection and tumor tissue was removed for more tests.
Results: The results showed that oral administration of L. rhamnosus decreased the growth rate of tumor (p<0.05). One reason for antineoplastic effect of lactobacilli is immune system enhancement. The results of delayed-type hypersensitivity show the stimulation of immune system and inhibition of tumor growth by this mechanism. In pathologic assessments probiotic administration increased the antineoplastic effect of cisplatin.
Conclusion: According to the findings of this study it can be expected that human studies also show the satisfactory effect of lactobacillus administration besides common therapeutic methods for cancer treatment.
Ma Hossein Pour Feizi , M Rojhan Nejad , N Pouladi, P Azarfam, V Montazeri, M Halimi,
Volume 20, Issue 4 (10-2012)
Abstract
Introduction: Survivin gene, as an apoptosis inhibitor, plays an important role in development of breast cancer. The differential expression of survivin in cancer versus normal adult cells as well as an association between high expression of survivin and aggressive tumors has led to use of survivin as a molecular marker for diagnosis and prognosis of tumors. The underlying mechanism of survivin over expression in cancers is not recognized yet. There is a probability that some polymorphisms in this gene can result in uncontrolled manner of this gene. The C-31G, a widely studied polymorphism in the survivin promoter, could de-repress the cell cycle- dependent transcription of survivin gene, resulting in over expression of survivin. In this hospital- based, case- control study, we aimed to investigate the correlation between the genetic polymorphism of -31G/C, surviving promoter, and breast cancer (BC) in North West of Iran.
Methods: In this case–control study, the -31G/C single nucleotide polymorphism (SNP) of survivin promoter in peripheral blood samples was collected from 94 breast cancer patients with pathologically confirmed BC and 82 healthy subjects. The data were analyzed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) and gene sequencing.
Results: Statistical analysis showed that within breast cancer patients, genotype frequencies were 50%, 46.8% and 3.2% for -31G/G, -31G/C and -31C/C genotypes respectively, whereas they were 50%, 45.1% and 4.9 % for -31G/G, -31G/C and -31C/C genotypes in healthy individuals. Also the frequencies for -31 C allele were 26% and 27% in the cases and controls respectively. No statistically significant association was found between breast cancer and the variant genotypes (CC and CG).
Conclusion: It seems that there was no significant association between -31G/C polymorphism, BC, and clinicopathological characteristics in the population of our study.
Sh Keyhanian, Ah Ghoreishi, Z Fotoukian, M Omrani-Nava, F Jassemi-Zergani, Mm Saravi,
Volume 21, Issue 3 (8-2013)
Abstract
Introduction: Fatty liver disease is characterized by deposition of fat droplets in the liver of patients. According to some epidemiological studies BMI, amount of fat intake from foods and high central fat are risk factors of breast cancer. This issue is one of the factors that cause high incidence of fatty liver in patients with breast cancer.
Methods: In this cross-sectional descriptive study, all patients with breast cancer were evaluated who referred to Imam Sajjad hospital of Ramsar during 2008 - 2011. After initial review, 100 patients were enrolled. Those who were treated by chemotherapy underwent abdominal Sonography for evaluation of fatty liver. Also, those patients who also received tamoxifen in addition to chemotherapy, underwent abdominal ultrasonography 6 months after taking tamoxifen. After that, relationship between treatment of breast cancer and fatty liver in studied patients was evaluated based on obtained information.
Results: The study results revealed that after chemotherapy, 30 (30%) patients were reported to have fatty liver. Out of 70 people that after chemotherapy did not have fatty liver, 62 patients received tamoxifen and after taking tamoxifen, 45.2% developed fatty liver. Using Chi Square test, there was a significant relationship between fatty liver after receiving tamoxifen, hyperlipidemia (p=0.011) and getting overweight (P =0.017).
Conclusion: As the findings indicated, treatment of breast cancer especially with tamoxifen is associated with increased risk of fatty liver, especially in women who have hyperlipidemia and are overweight.
S Ghobadzadeh, A Shams, G Eslami, A Mirghanizadeh,
Volume 21, Issue 3 (8-2013)
Abstract
Introduction: NKG2D receptor is one of the activator receptors of immune cells. In humans, NKG2D gene has two single nucleotide polymorphisms (SNP)(rs1049174G>C). According to other studies, GG SNP makes a high affinity receptor for NKG2D ligands whereas, CC polymorphism reduces NKG2D affinity for their ligands. The present study investigates NKG2D SNPs in patients with breast cancer in Yazd for the first time.
Methods: In this Case-Control study, 108 patients with breast cancer and 108 healthy women were selected from health Care centers of Yazd. Using specific NKG2D primers the gene proliferated and rs1049174G>C polymorphisms were determined by RFLP procedure.
Results: according to our statistic analysis, frequency of CC and GG genotypes in the patients were 44.4% and 13% respectively whereas, in the healthy donors the frequency was 71.3% and 6.5%. The difference between the frequency in healthy and patient groups was significant. On the other hand, the frequency of GC genotype in patients was considerably higher than the healthy women (PV, 0.0001). When the relative risk in the patients was determined, it was revealed that women with CC genotype have more susceptibility for breast cancer in comparison with healthy women (CI=95%, 1.31-2.24, OD=1.7).
Conclusion: The present study results showed that women with homozygote CC genotype have more chance of breast cancer in Yazd population.
S Kargar, B Alavi Farzane,
Volume 21, Issue 4 (10-2013)
Abstract
Introduction: HER-2 is a protein receptor of growth. Approximately 25% of breast cancers have overexpress HER-2. This study was designed to determine frequency of HER-2 overexpression in patients with breast cancer. Additionally, it intended to explore its relationship with some variables such as age, histopathologic diagnosis,tumor size, axillary lymph node involvement.
Methods: This analytic cross-sectional study was conducted on patients with breast cancer whose breast tissue specimen was referred to pathology laboratory of Shahid Sadooghi hospital of Yazd during 1389 and 1390.
Results: In our study, 147 patients were investigated who were all females with average age of 50.34 years with 13.3 years standard deviation. 54.5% of them were HER-2/neu positive and frequency of HER-2 was higher in patients with older age, larger size tumor, ductal carcinoma diagnosis and axillary lymph node involvement, though there wasn't any statistically significant difference between these variables and HER-2 overexpression.
Conclusion: In our study, frequency of Her-2 overexpression was more than other studies and frequency of HER-2 was higher in patients with older age, larger size tumor, ductal carcinoma diagnosis and axillary lymph node involvement but there wasn't any statistically significant difference between these variables and HER-2 overexpression. Regarding high frequency of HER-2 overexpression in this study that can be duo to genetic and ethnic variations, we recommend that HER-2/neu screening test should be done in all patients with breast cancers specially those with ductal carcinoma diagnosis, larger size tumor and axillary lymph node involvement.
Ma Hosseinpour Feizi, S Shiri Torkamani, E Babaei, M Ghanbarian, V Montazeri, M Halimi,
Volume 21, Issue 5 (12-2013)
Abstract
Introduction: Krupple-like factor4 is a transcription factor which is involved in many cancers including Breast cancer. Breast cancer is the most common malignancy among women. Due to the high prevalence of these tumors, there are ongoing efforts to find molecular markers which can recognize nontumoral from tumoral tissues. Therefore, the aim of this study was to evaluate the potential usefulness of KLF4 as a potential diagnostic and therapeutic molecular marker in breast cancer.
Methods: In the current study, 31 tumoral and 21 non tumoral adjacent tissues were evaluated. Transcription levels were measured by Semiquantitative Reverse Transciptase-Polymerase Chain Reaction and were normalized by the ß2m as an endogenous PCR control. Data was analyzed by spss software, one-way ANOVA and T-test.
Results: The results showed that: 1) KLF4 is over expressed in Breast tumors rather than adjacent normal tissues. 2) KLF4 is an oncogene in breast tumors (at least in IDC type). 3) The KLF4 expression levels are related significantly with nature of malignant breast tumors.
Conclusion: Findings do not confirm KLF4 as a diagnostic marker in classification and identification of tumoral tissues from non-tumoral ones in breast, but we can use this marker to identify at least 50% of invasive Ductal Carcinoma in breast and utilize it as a potential predictive factor to demonstrate severity degree in various tumors.
R Davoodi, S Esmailzadeh Bahabadi, Sh Najafi, M Mazaheri,
Volume 23, Issue 5 (8-2015)
Abstract
Introduction: Breast cancer is regarded as one of the most common female cancers in the world. Many efforts have been made to treat the breast cancer, among which surgery, radiation, and chemotherapy can be mentioned. There is an increasing interest regarding using herbal preparations as a promising source of new anti-cancer drugs because of their safety profile and efficient pharmaceutical potentials. Since citrullus colocynthis proposes some cytotoxic effects against cancer, the present study aimed to explore the effect of hydro alcoholic extract of C. colocynthis fruit on the viability and expression level of Caspase-3 in MCF-7 cell line.
Methods: In this laboratory study, the MCF-7 breast cancer cells were treated by different concentrations of Citrullus Colocynthis ethanol extract for 24, 48 and 72 h. The extract effect on cell viability was assessed by trypan blue staining. The RNA extraction was performed, and after the construction of cDNA, expression of Caspase 3 was analyzed via Real-Time PCR.
Results: The obtained results revealed a noticeable dose and time dependent reduction in viability of C. colocynthis in the treatment group compared to the control samples. Moreover Real-Time PCR results demonstrated that the expression of Caspase3 gene at 48 and 72 hours after extract treatment significantly increased compared to the control cells.
Conclusion: It can be concluded that citrullus colocynthis fruit extract can destroy cancer MCF-7 cells, which is resulted from an increase in caspase 3 gene expression.
F Moshtaghi, S Esmaeilzadeh Bahabadi , M Mazaheri, Sh Najafi, F Dahmardeh Ghalenoo ,
Volume 24, Issue 1 (4-2016)
Abstract
Introduction: There are problems such as treatment failure, drug resistance, heavy costs and other problems related to cancer treatment, which cause the plants to attract the interest of many researchers, because they have fewer side effects than chemical drugs. Studies have shown that Nigella Sativa has anticancer effects but the molecular mechanism of cell death of cancer cells is not clear. So the present study aimed to explore the effect of Nigella Sativa on the viability of MCF7 breast cancer cell line.
Methods: In this study, MCF7 cells were treated with different concentrations of hydro alcoholic extracts of Nigella sativa (0, 200, 400 µg/mL) for 24, 48 and 72 h. Effect of extract on cell viability and caspase-3 gene expression were analyzed by trypan blue staining and Real time PCR, respectively.
Results: The results revealed that cell viability significantly decreased in a dose and time dependent manner compare to the control (p < 0.05). Real time PCR results showed that the expression of Caspase-3 under different concentrations of extract (200 and 400 µg/ml) significantly increased compare to the
control (p < 0.05).
Conclusion: It seems that Nigella sativa caused cell death by increasing of caspase-3 gene expression
M Younesi, Ma Hosseinpour Feizi , N Pouladi,
Volume 24, Issue 3 (6-2016)
Abstract
Introduction: A common polymorphism 4G/5G in the promoter region of the Plasminogen activator inhibitor-1 (PAI-1) gene has been reported to influence the expression levels of PAI-1. According to the evidence, progression of breast cancer can be associated with elevated levels of PAI-1, it seems that evaluation of a possible correlation between the polymorphism and clinical status of breast cancer patients is reasonable.
Methods: This descriptive-analytical study included 160 unrelated patients from North West of Iran. According to established clinical criteria, these paitients were diagnosed with breast cancer. Based on previous study, PAI-1 4G/5G had been determined. In order to investigate the association of this polymorphism with clinicopathological features Fisher’s exact tests and SPSS software was used with a significance level of 0.05.
Results: All declared features of breast cancer regarding PAI-1 4G/5G polymorphism were investigated. Results indicated that PAI-1 4G/5G polymorphism positive correlation with several traditional prognostic factors, including tumor size, lymph node metastases and tumor stage.
Conclusion: Data showed that the patients with 5G/5G genotype are more susceptible to the development of breast cancer, while the paitients with 4G/4G and 4G/5G genotypes show lower sensitivity to the breast cancer. Therefore, the 4G allele likely has a protective role against the development of breast cancer in this cohort.
Frida Seyedmir , Kamal Mirzaie, Morteza Bitaraf Sani,
Volume 25, Issue 4 (7-2017)
Abstract
Abstract
Introduction:
Decision tree is the data mining tools to collect, accurate prediction and sift information from massive amounts of data that are used widely in the field of computational biology and bioinformatics. In bioinformatics can be predict on diseases, including breast cancer. The use of genomic data including single nucleotide polymorphisms is a very important factor in predicting the risk of diseases. The number of seven important SNP among hundreds of thousands genetic markers were identified as factors associated with breast cancer. The objective of this study is to evaluate the training data on decision tree predictor error of the risk of breast cancer by using single nucleotide polymorphism genotype.
Methods:
The risk of breast cancer were calculated associated with the use of SNP formula:xj = fo * In human, The decision tree can be used To predict the probability of disease using single nucleotide polymorphisms .Seven SNP with different odds ratio associated with breast cancer considered and coding and design of decision tree model, C4.5, by Csharp2013 programming language were done. In the decision tree created with the coding, the four important associated SNP was considered. The decision tree error in two case of coding and using WEKA were assessment and percentage of decision tree accuracy in prediction of breast cancer were calculated. The number of trained samples was obtained with systematic sampling. With coding, two scenarios as well as software WEKA, three scenarios with different sets of data and the number of different learning and testing, were evaluated.
Results:
In both scenarios of coding, by increasing the training percentage from 66/66 to 86/42, the error reduced from 55/56 to 9/09. Also by running of WEKA on three scenarios with different sets of data, the number of different education, and different tests by increasing records number from 81 to 2187, the error rate decreased from 48/15 to 13/46. Also in the majority of scenarios, prevalence of the disease, had no effect on errors in the WEKA and code.
Conclusion:
The results suggest that with increased training, and thus the accuracy of prediction error decision tree to reduce the risk of breast cancer increases with the use of decision trees. In Biological data, decision trees error is high even with a 66/66% training. On the other hand by increasing the number of SNP from 4 to 7 decision tree, decision tree error dramatically decreased at 70/1% training. In general we can say that with increased training and increasing the number of SNP in the decision tree, the prediction accuracy increased and errors reduced. In the CODING and WEKA, percentage of disease prevalence had no significant effect on errors,” Because of selecting set of training and testing by systemic method “.
Parisa Poorheydar, Mohammadali Hosseinpour Feizi, Reza Safaralizadeh, Nasser Pouladi, Reyhaneh Ravanbakhsh Gavgani,
Volume 25, Issue 4 (7-2017)
Abstract
Introduction: Breast cancer is the most common malignancy worldwide, which affects women. Also, this disease is one of the most frequent malignancies among women in Iran. Apoptosis is a known mechanism against cancer, which has intrinsic and extrinsic pathways. One of these extrinsic pathways, is Fas receptor-ligand system, which plays a key role in apoptotic signaling in many cell types, particularly in immune system cells. Disruption of this pathway will cause in tumorigenesis. Furthermore, other studies have shown polymorphisms in genes related to this pathway, which affect their expression in different cancers. Polymorphisms in FasL gene can influence its expression and cause breast cancer. This study aimed to evaluate association of FasL IVS2nt-124A/G polymorphism and breast cancer susceptibility.
Methods: This case-control study was carried out on 100 specimens of breast cancer patients and 100 specimens of healthy people from East Azarbaijan province. After DNA extracting, genotyping was performed using ARMS-PCR method.
Results: Genotype distribution for healthy controls and cases for AA genotype was 44.0% and 60.0% respectively, and a significant statistical difference was observed (P<0.05). AG genotype percent in controls was higher than in cases (50.0% and 33.0%) and a significant statistical difference was observed (P<0.05). About GG genotype and allelic distribution, there was no significant statistical difference between case and control groups (P>0.05).
Conclusion: Present research findings demonstrate that AA genotype is associated with increased risk of breast cancer among breast cancer patients in East Azarbaijan.
Hadi Abdolrezaee, Somayeh Reiisi, Leila Rouhi ,
Volume 25, Issue 6 (9-2017)
Abstract
Introduction: Breast cancer remains the prominent cause of mortality in women. Several biomarkers are used to evaluation the response and targeting to therapy. Tumor suppressor candidate 1 (TUSC1) gene was newly identified as a probable tumor suppressor in human cancers. Nevertheless, the expression and potential function of TUSC1 in breast cancer stay undecided. Therefore, this study aimed the evaluation of TUSC1 gene expression in breast tumor samples.
Methods: In this case-control study, 40 formalin-fixed paraffin embedded (FFPE) tumoral of breast cancer and 40 healthy tissues were enrolled. Followed were informed consent and completing clinical information for all samples. Total RNA was extracted and complementary DNA (cDNA) was synthesized. The relative gene expression was determined using quantitative real-time RT PCR (qRT-PCR) and evaluated by method.
Results: The expression of TUSC1 gene was lower in tumor tissue compared to the healthy tissue adjacent and it was statistically significant (P =0.0003). Also, in metastatic state gene expression significantly decreased (P=0.027).
Conclusion: Our study revealed that the expression of TUSC1 is lower in breast cancer. Subsequently, using considering all the data about the expression of TUSC1 gene from some cancers (e.g. Lung, Hepatocellular and gastric), it could be suggested that the TUSC1 gene might act as a tumor suppressor in breast cancer and influenced in metastasis. Therefore, supplementary studies should be done to elucidate the exact mechanism of action of the gene in tumor-genesis
Hadis Ahmadirad , Mohammad Reza Hajizadeh Hajizadeh , Mehdi Mahmoodi , Mohamadreza Mirzaee , Fahime Mohammadain Shahrbabaki , Mostafa Soltaninejad ,
Volume 25, Issue 7 (10-2017)
Abstract
Background: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.
resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined effect of resveratrol on the expression of genes referred to the two classes of breast cancer cell lines
Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression.
Results: The results showed that resveratrol decrease in gene expression APOBEC3B and DNMT-1 And increased expression of TET_1 in both of cell lines. .
Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects through epigenetic mechanisms applied. However, the final decision needs to be further investigation.
Setareh Pourhavashemi, Zahra Bamzadeh, Leila Rouhi, Noosha Zia-Jahromi,
Volume 26, Issue 5 (8-2018)
Abstract
Introdution: Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native Pseudomonas sp. UW4 metabolite to find new compound.
Methods: This experimental study was performed in Shahrekord Islamic Azad University from April 2015 to August 2015. First SK-BR-3 human breast cancer cells with different concentrations (5, 10 and 20 mg/ml) were treated with produced metabolite by native Pseudomonas sp. UW4 for 24 and 48 hours. Cell viability was assessed by MTS assay. Then, p53 expression was detected by RT-Real Time PCR. For statistical analysis, SPSS16 software and one way ANOVA test were used.
Results: Treatment with produced metabolite by the native Pseudomonas sp. UW4 showed the decreases the viability of cells in a time and dose dependent manner, the most effective concentration of this substance was 20 mg/ml and 48 h after treatment. Also, an increase in p53 gene expression, significantly in 10 and 20 mg/ml after 24 h and 5, 10 and 20 mg/ml after 48h was observed (P<0.05).
Conclusion: Produced metabolite by native Pseudomonas sp. UW4 could be used for treatment of SK-BR-3 breast cancer.
Seyed Mohsen Miresmaeili, Seyed Mehdi Kalantar,
Volume 26, Issue 8 (1-2019)
Abstract
Introduction: Breast cancer is the most common malignancy in women worldwide. BRCA1 is a tumor suppressor gene that is involved in DNA-damage repair. One of the significant risk factors of breast cancer is the family history. BRCA1 gene consists of 24 exons that encode a protein with 1863 amino acids. Exon 11 is the largest exons and most of the disease-linked mutations have been found in it. Inherited mutations in the BRCA1 gene are constituted for the major hereditary breast cancer cases. Deficiency in BRCA1 gene was considered to be a high risk in the families with breast cancer. The goal of this study was to evalute mutation in BRCA1 gene in the patients with breast cancer.
Methods: In this study, blood samples were collected from 200 breast cancer patients. 40 patients suffer from hereditary breast cancer, after DNA extraction, exon 11 of BRCA1 genes were evaluated by PCR-SSCP technique followed by direct sequencing and the result of the changes was evaluated by in silico analysis.
Results: The samples, showing mobility shift on SSCP analysis, were used for direct DNA sequencing, two new missense mutations c.3059C>T (p.Pro1020Leu) and (p.Thr1025Ile) c.3074C>T were detected in the patients with hereditary breast cancer. In silico analysis on protein function revealed that the mutation c.3074C>T effected on protein structure and predicted to be possibly damaging.
Conclusion: Screening of the BRCA1 gene mutation can help to manage the treatment of the patients and their families. Therefore, for families exposed to cancer, genetic screening can help family members know if they need tests to look for cancer early, or if they should take steps to reduce their risk.
