Showing 6 results for Bax
Mohammad Naser Karimi, Roghayeh Abbasalipourkabir, Zahra Arab Sadeghabadi, Dr Nasrin Ziamajidi,
Volume 25, Issue 7 (10-2017)
Abstract
Introduction: One of the complications of diabetes mellitus is induction of apoptosis in liver tissues. Activation of caspase 3 caused apoptosis in the cells. Garlic is a most common plant in diet that its effect on apoptosis was not determined. In this study the effects of this extract on Bax and Bcl-2 gene expression and the activity of caspase 3, were determined in type 1 and 2 of diabetes in rats.
Methods: Wistar rats divided into six groups included normal control rats, diabetes type 1 (D1), diabetes type 1 treated with garlic extract (D1G), diabetes type 2 (D2), diabetes type 2 treated with garlic extract (D2G) and garlic control (G). At the end of treatment time, liver tissues were removed quickly and stored
in -70 °C. For evaluation of gene expression, RNA extraction, cDNA synthesis and Real time PCR were done. The activity of caspase 3 was determined with flourometric method.
Results: The level of gene expression of Bax and Bcl-2, did not change significantly in different groups. The activity of caspase 3 in D1 rats, increased significantly compared to the control rats(p=0.003). The activity of this enzyme was high in D1G compared to control rats yet (p=0.021). In D2, D2G and G groups, caspase activity was not changed in comparison with the control rats
Conclusion: Diabetes mellitus type1 caused apoptosis in rat livers, but garlic extract cannot ameliorate it.
Samad Safrazadeh Gargari, Hassan Matin Homaei, Mohammad Ali Azarbayjani,
Volume 26, Issue 4 (7-2018)
Abstract
Introdution: The research has been indicated that reactive oxygen species induced the apoptosis of cardiomyocytes. However, this mechanism has been unclear. The aim of this study was to investigate the effects of concurrent training on some of the heart apoptosis variables
(BAX,BCL-2) following by the injection of different H2O2 dose in Wistar rat males.
Methods: 50 male rats were randomly assigned to 5 groups with 10 rats in which group. Groups included: group (1): control group (C), group (2, 3): injection of 1 and 2ml H2O2, group (4,5): exercise and injection of 1and 2 ml H2O2. Exercise groups have been run on treadmill for four days during 8 weeks at moderate intensity. 24 hr after the last exercise and in anesthetic state all rats have been knockouted to determine bax and bcl2 proteins ratio. For measuring the BAX and BCL-2 proteins were used by ELISA technic and total protein were determined by brad ford technic. Data were analyzed by SPSS 17, one ways ANOVA was used to analysis of data at the level of p≤0/05.
Results: The results have been indicated that after two month continues training no significant difference in BAX, BCL-2 proteins and BAX/BCL2 ratio in exercise and H2O2 groups in compared by control groups.
Conclusion: based on the result of this study if the 8-week continues training has been followed by H2O2 injection with both of one and two ml H2O2 concentration, it may not have induced apoptosis cardio myocyte in rats. And it may adjusted the synthesis and degradation myocardial
Sara Afzali, Abbas Doosti, Mansour Heidari, Nahid Babaei, Parvaneh Keshavarz,
Volume 27, Issue 6 (10-2019)
Abstract
Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-2 و Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells.
Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7 cells transfected with the pcDNA3.1(+)-seb (recombinant) and pcDNA3.1(+) (non-recombinant) plasmids and were selected by culturing in a selective medium of RPMI- 1640 containing 600 μg / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used.
Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032).
Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.
Alireza Safarnezhad, Maghsoud Peeri, Mohammad Ali Azarbayjani, Maryam Delfan,
Volume 28, Issue 7 (10-2020)
Abstract
Introduction: Regular exercise training with alternating volume fluctuatesblood glucous levels and by regulating signaling in gene expression reduces in myocardial cell apoptosis in diabetic patients. The purpose of this study was the effect of 8 weeks of high intensity interval training on the gene expression of BAX and BCL-2 in the left ventricle of diabetic rats.
Methods: The present study was a quasi-experimental one. 14 male diabetic rats were divided into 2 groups of 7; high intensity interval training (HIIT) and control (C) groups. Diabetes was induced in a pellet with a high-fat diet (30% fat and 25% fructose) for 16 weeks. Twenty-four hours after the last training and recovery session, the rats were sacrificed and their left ventricle was extracted. Glucose oxidase was used to measure glucose in plasma and insulin resistance using HOMA-IR method.. PCR-Real time was used to determine the expression of BAX and BCL-2 genes and the comparison of the groups by Independent T test was performed by Graph pad prism at alpha level of 0/05.
Results: Results showed that, BAX gene expression was significantly decreased in the HIIT group compared to the C group (P=0/0001). BCL-2 gene was significantly increased in the HIIT group compare to the control group (P=0/0001). Insulin resistance index and plasma glucose showed a significant decrease in the training group (P=0/01) (P=0/021). Weight did not change significantly in any of the groups.
Conclusion: Based on the findings, 8 weeks high intensity interval training can be reduced apoptosis in the left ventricle of Diabetic mice by decreasing the BAX gene expression and increacing the BCL-2 in myocardial and might improve diabetes cardiomyopathy.
Maryam Akbarzadeh, Farkhondeh Nemati, Fatemeh Shaki, Abbas Ali Dehpouri Gouibari, Ramin Ataee,
Volume 30, Issue 4 (7-2022)
Abstract
Introduction: Methadone is a substance that is widely used in the substitution treatment of opiate addiction. This study aimed to evaluate the protective effects of melatonin on apoptosis induced by transfer of transplacental methadone in mice.
Methods: This study was an experimental study aimed to assay apoptotic effect of placenta transferred methadone on brain, liver and kidney tissues and to study protective effect of melatonin in an embryo model in mice. Bulb-C mice weighing 25-30 g were kept in four shelves (3 females and one male) and checked daily for the sterile cycle. For mice with vaginal plugs, the first day of pregnancy was considered. After confirmation of pregnancy, female mice were divided into 5 groups of 6, which included the control group, methadone (10 mg / kg) and melatonin groups in three doses (2, 4, 6 mg / kg / day) by IP injection for half an hour before prescribing methadone. This prescription was given for 10 days from the beginning of pregnancy. After the infants were born, their liver, brain, and kidneys were removed and they underwent immunohistochemical tests for apoptotic expression of Bcl2, BAX, and Caspase3 proteins. The results were analyzed by SPSS version 22 statistical software and Chi-square (Chi 2) and Fisher tests (P <0.01).
Results: This study showed that melatonin at dose 4 and 6mg/kg has decreased apoptotic protein BAX and Caspase9 and increased anti-apoptotic protein, Bcl2 expression approximately, but these results were not significant statistically (P>0.05). In addition, for dose of 0.2 mg/kg of melatonin, there was not any apoptotic effect.
Conclusion: Our findings showed that melatonin has almost a protective effect against apoptotic effect induced by placental transfer of methadone especially via its increasing effects on Bcl2, but this anti-apoptotic effect was not significant and needed for more precise studies.
Elham Hemati, Hossein Sazegar,
Volume 32, Issue 7 (10-2024)
Abstract
Introduction: Accumulating evidence has revealed that inducing apoptosis is an important strategy to control excessive breast cancer cell proliferation. In this study, the supernatant effect of the probiotic strain of Lactobacillus Sakei on Expression Level of Apoptosis-Related Genes (Bax/Bcl2) in Breast Cancer Cell Line (MCF-7) was investigated.
Methods: In the experimental study, MCF-7 breast cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 1, 2, 3, 4 and 5 mg/ml concentrations of sakei supernatant and incubated at 24, 48 and 72 hours. The MTT assay was used to measure cytotoxicity effect according to kit manufacturer's protocol in all three incubation times. MCF-7 cells with concentration of sakei supernatant at IC50 point (5 mg / ml) to examine the expression of genes Bax and Bcl2 were incubated for 72 hours. Real-Time PCR was performed to analyze the changes in the expression of Bax and Bcl2 genes.
Results: The results of this study indicated that bioavailability of MCF-7 cell line reached the lowest value at concentration of 5 mg/ml compared to the control group. In addition, MCF-7 cell line treatment with Lactobacillus sakei supernatant at a dose of 5 mg/ml and 72 h incubation time, showed significantly increased expression of BAX (p-value= 0.0033) and significantly decreased expression of BCL-2 (p-value= 0.0278).
Conclusion: The results indicate that Lactobacillus sakei supernatant can reduce the bioavailability of breast cancer cells by inducing apoptosis pathway.
