Showing 3 results for salehnia
M Yaftiyan, M Salehnia, Aa Pourfatollah,
Volume 13, Issue 4 (Atumn 2005)
Abstract
Introduction: Considering the importance of co-culture in differentiation of embryonic stem cells, the aim of
this study was evaluation of the effect of co-culturing fetal liver stroma cells with P19 cells on the line of
differentiation.
Materials and Methods: For this purpose, P19 cells were cultured directly in semisolid medium. These cells
proliferated and primarily differentiated to colonies know as embryoid bodies (EBs) after 8-12 days.
The Ebs cells were trypsinized and dissociated to single or double cells.
Then these cells were co-cultured on the mouse fetal liver feeder layer in the absence of exogenous factors.
After 14-18 days, the colonies were studied morphologically by benzidine and giemsa staining and also
counted under invert microscope.
Results: The percentages of benzidine positive (or erythroid) and negative colonies were 94% and 6%
respectively and also the cells of colonies were studied by Giemsa staining. Results showed that they were
myeloid or lymphoid type cells.
Thus, the results show that in the presence of mouse fetal liver feeder layer, the number of erythroid colonies
was increased.
Conclusions: Therefore, this technique may be effective for differentiation of stem cells from different
sources into hematopoietic cells and can be used in future for human cell therapy.
M Salehnia, A Al Yassin, M Agha Hosseini, L Safdarian, A Khademi, H Saidi, Z Rezaieyan, Sh Byranvand,
Volume 14, Issue 3 (Autumn 2007 2006)
Abstract
Introduction: The aim of this study was to evaluate the correlation between serum levels of ovarian and gonadotropin hormones, age and number of follicles with follicular alkaline and acid phosphatase activity in infertile women under controlled ovarian hyperstimulation.
Methods: After collection of follicular fluid and calculation of the number of follicles, the specific activity of alkaline (ALP) and acid phosphatase (ACP) was determined according to the total protein in 19 women at the time of puncture. Also at that time, the levels of progesterone, estradiol, and follicle stimulating hormone (FSH) and leuteinizing hormone (LH) of their sera were measured. The correlation of follicular ALP and ACP with each serum hormone levels, women age and number of follicles was calculated using non-parametric analysis.
Results: The ALP has a correlation with progesterone (P=0.01) levels but doesn’t have any correlation with the other factors. However, the ACP activity has a correlation not only with follicular number but also with estradiol and progesterone levels (P=0.05).
Conclusion: Thus ACP activity is more affected by ovarian hormone than ALP and it can affect the ovarian microenvironment and oocyte development.
R Fathi, M Rezazadeh Valojerdi, M Salehnia, M Totonchi, N Deheshkar Farahani, B Ebrahimi, F Shabani, P Borjian Boroujeni,
Volume 22, Issue 3 (Jul- Aug 2014)
Abstract
Many changes of female reproductive system are based on the vascular activity and blood flow. In ovarian follicular development, re-anastomosis is critical and permits cells and oocytes to utilize nutrients as well as the hormones. Moreover, it plays a main role in corpus luteum formation. Female reproductive system undergoes several angiogenesis programs which are considered essential in order to reach a follicle to preovulatory stage and to set up a network between granulosa cells and blood vessels through the theca layer. Up to the first 3 days after transplantation and reanastomosis start, a large number of follicles especially advanced types, begin an early death due to their severe dependence on nutrients of blood supply. Loss of the nutritional sources of granulosa cells leads to a decrease in survival and supporting factors and thus, cell death increases. Considering the importance of this issue, the present study intends to investigate the activity of angiogenic and apoptotic factors in transplanted ovarian tissue.
