Showing 12 results for khalili
Mb Khalili, Mr Sharifi,
Volume 12, Issue 1 (spring 2004)
Abstract
Interoduction: Gasteroenteritis is one of the problems worth consideration all over the world. It is one of the important causes of mortality, especially in children < 5 years of age, in developing countries including Iran. The aim of this descriptive study was to determine the demographic conditions influencing the presence of causative bacteria and protozoa, followed by antibiograms of isolated bacteria from stool samples of patients with gasteroenteritis referring to Nikoopour Clinic in the city of Yazd, Iran from 1998 – 2001.
Materials and method: A total of 470 samples were microbiologically examined by direct method, culture and then antibiogramed. In order to isolate the possible bacteria, differential and selected media were used. Also, wet – mount technique was applied for detection of protozoa.
Results: Results revealed that 272 samples (57.9%) were infected by pathogenic bacteria or protozoa. 138 (50.8%) pathogenic specimens were from male patients and the remaining 134(49.3%) were from female patients. Isolated species were: Enteropathogenic E.coli 117(43%), Shigella 51(18.8%), Salmonella.interetidis 25(9.2%), C.jejuni 16(5.9%), Giardia lambdia 51(18.8%) and Amoebae spp 12(4.4%). The most commonly detected shigella species was dysenteriae, (74.5%) while boydii with 2% was the least common type observed in the specimens. Except shigella, all the other bacteria were more common in males than female, but insignificant statistically. In order to determine the sensitivity and/or resistance of pathogenic bacteria, antibiogram test was performed using selected antibiotic disks such as Ampicillin, Nalidixic Acid, Ciprofloxacin, Gentamycin and Sulfamethaxazole.
Conclusion: Results revealed that some patients were probably infected by pathogenic factors other than bacteria or protozoa. Since all viruses and parasites are almost resistant to antibiotics and on the other hand, administration of antibiotics may lead to resistance of bacterial agents, it seems necessary that the stool samples should be cultured and antibiogramed before treatment with antibiotics. The most common bacterial agent isolated in this study was E coli enteropathogens and the most effective antibiotic against it was Nalidixic acid.
Ag Abbasi Sarchashmeh, Ma Khalili, M Anvari,
Volume 12, Issue 2 (summer 2004)
Abstract
Introduction: Spinal cord injury (SCI) is a serious public health problem which seriously affects the victim, family, and even the society. Research studies have shown that 80% of SCI victims are men. In recent years, there have been extensive research works on the effect of SCI (acute and/or chronic) on fertility potential of sperm and spermatogenesis in laboratory animals. SCI may disturb the spermatogenic cell lines in laboratory animals. The objective of this experimental study was to investigate the effect of chronic spinal cord injury (CSCI) on sperm parameters in adult rats.
Materials & Methods: Adult Wistar rats weighing between 225-275g were divided into 3groups of control (n=5), sham (n=10), and experimental CSCI (n=10). No surgery was done on control animals. Only laminectomy was done in the sham animals at T10. CSCI was developed in experimental rats using 10g weight dropped 5cm above the exposed T10 level. All animals were sacrificed 50 days post experiment to extract epididymal samples. Sperm parameters of count, motility, morphology, as well as number of round cells were evaluated with the aid of Makler chamber and Geimsa staining.
Results: Progressive motility was significantly reduced in CSCI group (P<0.05). The percentage of normal morphology of spermatozoa was 99.0±1.0 in control rats which was significantly reduced to 74.90±37.64 in CSCI animals In addition, sperm counts in control and CSCI rats were 69.20±12.43 and 25.0±13.68, respectively (P<0.01). Round cell concentration was increased in CSCI group as compared to controls.
Conclusion: The results suggest that reduction in parameters of progressive motility, morphology, as well as sperm count following CSCI in rats may disturb the fertility potential of spermatozoa.
F Saadlou, Ma Khalili, A Hazeghi,
Volume 15, Issue 2 (Summer 2007)
Abstract
Introduction: In Iran, thousands of young individuals become victims of head injury annually. Head injury can damage neuronal cells which may in turn complicate the recovery of the patients. Also, the increase of iron in cerebrospinal fluid (CSF) following head injury may contribute to the hypoxic-ischemia brain damage by catalyzing the formation of free radicals (Harber-Weiss Rection). The object was to study the correlation between the level of iron in CSF with cerebral tissue damage after experimental head injury in adult male rats.
Methods: A total of 27 Wistar rats were randomly divided into 3 groups of control (n=7), experimental I (n=9), and experimental II (n=11). Head injury was induced with a 300g weight dropped on the skull from a distance of 1 meter. CSF samples were withdrawn from controls with insertion of a Hamilton needle throught atlanto-ocipital (OA) membrane. Also, CSFs were collected from experimental animals I and II at 30 min and 60 min after induction of head injury, respectively. All CSF samples were measured with Atomic Absorption Spectrometry. Also, biopsy samples from parietal lobe of brain were fixed in 10% formalin and stained with Hemotoxylin-Eosin for morphological evaluation.
Results: The level of iron in CSF of control group was 2.95±31ppm. The level of iron in experimetanl groups of I and II increased to 6.14±1.01 (P>0.05) and 14.72±2.94 (P<0.001), respectively. The cerebral tissue of group I consisted of abnormal dark neurons which were scattered among normal neurons. However, the majority of neuronal cells were damaged in experimental group II.
Conclusion: The results showed that the level of iron in CSF as well as neuronal cell architecture was time-dependent after experimental head injury. This may cause a non-reversible brain injury with release of free radicals in CSF following head injury.
J Hossein Zadeh, Mb Khalili, Ms Golvardi, M Sadeh, Mh Sadr-Bafghi, Ps Mohseni- Meybodi,
Volume 16, Issue 1 (Spring 2008)
Abstract
Introduction: Many post-surgical wound infections in hospitals cause morbidity and morality of patients and these are usually transmitted via hands of surgical personnel. The aim of the present study was to detect and antibiogram the bacterial agents following scrubbing of hands of surgical personnel before operation.
Methods: Hands of 134 personnels of operation room were swabbed following scrubbing with antiseptic Betadine solution. Swab samples were inoculated on selective and differential media such as blood ager, McConky and manitol salt agar(MSA). Following incubation of media at 37c° for 24hr, bacterial species were identified using differential related tests. The isolated species were than antibiogramed and the results together with other data was analysed by SPSS software program.
Results: Of the total of 134 cases, 81(60.4%) were male and 53(39.6%) female. The mean scrub time for each person was (206.1+/-103.2) seconds 6 to 60 seconds base change. Increasing time of scrub was significantly correlated with decreasing rate of bacteria (P=0.003), (R=-0.254). Contamination was present in 129(96.3%) cases following scrubbing. Maximum contamination was observed in nails (92.5%). Average number of bacteria for each individual was between 0 and 159. 62.6% of isolated bacteria were non- staphylococci and 7.7% were S. aureus. Vancomycin and ceftizoxim were the most sensitive, while penicillin was the least sensitive antibiotic.
Conclusion: Results revealed that hand contamination was more than the expected standard level. Therefore, regarding the critical task of surgical personnel, training of all operation room staff is highly recommended to minimize the rate of contamination.
M Khalili, M Miresmaeili, T Malakoutian, Y Mostafavi Pour-Manshadi,
Volume 19, Issue 4 (Sep-Oct 2011 2011)
Abstract
Introduction: Ecstasy or 3,4-Methylenedioxymethamphetamine(MDMA) is a psychotropic and addictive substance that young people tend to use it to reduce their psychological and social tensions. The purpose of this study was to assess the influence of ecstasy consumption on the fetus of pregnant mice during the second and third weeks of pregnancy.
Methods: 20 adult female mice were randomly selected(5 for control group and 15 for experimental group). Two intraperitoneal injections of ecstasy(5mg/Kg) was used in the experimental group, on 7th and 14th days of pregnancy, while, in the control group, only distilled water was injected intraperitoneally. On 18th day of pregnancy, mice were placed in separate cages. The condition of palate, skull, external ear, eye, fingers and toes and sindactily, weight, and fertility potentials of newborn mice were studied using stereo microscope.
Results: From 163 newborn mice in two groups, no abnormalities were observed in the skull and the external ear. There wasn’t any significant difference between male and female sex ratio between two groups (p=.08). Hypoplasia of the fingers was significantly different between the two groups(p<0.001). The frequency of sindactily was not significantly different between two groups(p=0. 11). Female fertility potential was significantly different between two groups(p<0.001).
Conclusion: Adminstration of ecstasy during pregnancy may affect the organogenesis and fertility potential of newborn mice. Therefore, more studies are needed in this regard.
Mrs N Mazaheri, Dr A Karimi, Dr H Salavati, Dr S Rezaei Zarchi, Dr S Khalilian, Mrs R Rezaei Ranjbar Sardari,
Volume 22, Issue 4 (Sep-Oct 2014)
Abstract
Introduction: In spite of increasing usage of metal nanoparticles, few studies have been conducted on their side effects, particularly under in-vivo conditions. Hence, the present study aimed to assess the effect of magnesium oxide nanoparticles (MgONPS) on the liver and kidney function of rats in vivo
Methods: Concentrations of 62.5, 125, 250 and 500 ppm of MgONPS (10-15 nm size) were intraperitoneally injected into rats, and then the liver and kidney function were investigated.
Results: The study results revealed that MgONPS caused different changes in liver enzymes. In fact, the AST and ALP values were significantly increased compared with the control group, whereas the levels of ALT, Urea and Creatinine did not demonstrate any significant differences. In addition, no histological disorders were observed in the kidney tissue, in contrary to liver tissue in which some alternations were observed such as apoptosis and proliferation of small bile ductules indicating damage of tissue in expose of high doses of MgONPS.
Conclusions: The study findings indicated that magnesium oxide nanoparticles in higher doses of
250 ppm can have toxic effects on the liver, therefore their toxicity should be considered, while applying them in different fields of industries.
G Khalili-Zadeh-Mahani , Mr Pajoohan, V Derhami , A Khoshnood ,
Volume 24, Issue 4 (Jul 2016)
Abstract
Introduction: Reducing unnecessary laboratory tests is an essential issue in the Intensive Care Unit. One solution for this issue is to predict the value of a laboratory test to specify the necessity of ordering the tests. The aim of this paper was to propose a clinical decision support system for predicting laboratory tests values. Calcium laboratory tests of three categories of patients, including upper and lower gastrointestinal bleeding, and unspecified hemorrhage of gastrointestinal tract, have been selected as the case studies for this research.
Method: In this research, the data have been collected from MIMIC-II database. For predicting calcium laboratory values, a Fuzzy Takagi-Sugeno model is used and the input variables of the model are heart rate and previous value of calcium laboratory test.
Results: The results showed that the values of calcium laboratory test for the understudy patients were predictable with an acceptable accuracy. In average, the mean absolute errors of the system for the three categories of the patients are 0.27, 0.29, and 0.28, respectively.
Conclusion: In this research, using fuzzy modeling and two variables of heart rate and previous calcium laboratory values, a clinical decision support system was proposed for predicting laboratory values of three categories of patients with gastrointestinal bleeding. Using these two clinical values as input variables, the obtained results were acceptable and showed the capability of the proposed system in predicting calcium laboratory values. For achieving better results, the impact of more input variables should be studied. Since, the proposed system predicts the laboratory values instead of just predicting the necessity of the laboratory tests; it was more generalized than previous studies. So, the proposed method let the specialists make the decision depending on the condition of each patient.
Abbas Khalili,
Volume 29, Issue 10 (1-2022)
Abstract
Introduction: Primary immunodeficiencies (PID) are rare heterogeneous disorders with defects in which one or more components of the immune system are malfunctioning. Clinical presentations of the patients according to type of immunodeficiency are variable. The majority of these patients are susceptible to infections depending on the type of disorder. In these patients, one of the most important and common symptoms is a skin manifestation that in many cases helps to diagnose the disease. Skin symptoms can include infectious-inflammatory-autoimmune-allergic manifestations and malignancies. In some cases, skin involvement can be the initial manifestation of immunodeficiency diseases, so understanding the relationship between the type of primary immunodeficiency and the type of skin involvement is very important in diagnosing the disease. The majorities of skin diseases are not pathogenomonic in primary immunodeficiencies and may be seen in other diseases with normal levels of immunity. However, there are numerous skin findings that are so characteristic of immunodeficiency diseases that it is necessary to evaluate the immune system.
Conclusion: Skin is an organ that may be involved in many diseases, including primary immunodeficiency. Sometimes skin is the first organ involved in immune deficiencies. Therefore, recognizing skin manifestations in these patients is one of the most important factors in early diagnosis of these people.
Fahimeh Sabet, Fariba Behnamfar, Narjes Khalili, Farnaz Baba Haji Meybodi,
Volume 29, Issue 11 (2-2022)
Abstract
Introduction: Despite the significant relationship between human papillomavirus 16 and 18 subtypes and severe cases of cervical cancer, the assessing the frequency of high-grade cervical intraepithelial neoplasms in the patients infected with high-risk human papillomavirus other than 16 and 18 has been less studied in Iranian population.
Methods: In this cross-sectional study, the patients referred to Oncology Clinic of Shahid Beheshti Hospital in Isfahan, Iran were selected by positive HPV-typing test through Cobas HPV test and high-risk papilloma virus in 2018 by convenience sampling method. Pap smear was first taken from the patients in the usual way, then they underwent colposcopy, and a biopsy was performed on the lesion during colposcopy. If colposcopy was normal, biopsies were randomly taken from 4 quadrants (12, 3, 9, 6 hours) and, if necessary, endocervical curettage was performed. Samplings were taken by the pathologist of the study center and the prevalence of CIN2 and CIN3 was reported. ANOVA test was performed to compare quantitative variables. Chi-square test was used to compare qualitative variables. SPSS version 17 software was used for statistical analysis of data. Significance level was considered less than 0.05.
Results: In this study, 105 individuals with high-risk HPV other than 16 and 18 were evaluated. In the biopsy performed according to the standard diagnostic method, the prevalence of severe form of precancerous lesions (CIN 2, 3) was 9.6% and the most common types of HPV in the patients with CIN 2, 3 were 52 with a frequency of 40% and type 58 with a frequency 30%.
Conclusion: Due to the significant prevalence of severe (precancerous) lesions in these patients, it is necessary to review the current protocols.
Abbas Khalili,
Volume 31, Issue 11 (2-2024)
Abstract
Introduction: Primary immunodeficiencies are relatively rare and heterogeneous disorders in which one or more component of immune system have genetic defect. The majority of the patients suffer from frequent infections with different microorganisms according to the type of disorder they have. Some people with primary immunodeficiency are susceptible to malignant diseases. The type of malignancy depends on the type of primary immunodeficiency, age of the patient and the type of virus infection. Many advances have been made in understanding of molecular mechanisms in humeral and cellular immune defects. Furthermore، our knowledge has been increased about the incidence of infections and tumors in primary immunodeficiency and this helps us in early diagnosis of disorders and their complications. Several evidences support the theory that the immune system plays a major role in the occurrence of many malignant diseases. Articles have been searched on Google Scholar، PubMed، Web of Science، and Scopus databases. In this review، the latest and most reliable articles related to the subject that had been published in English language were used.
Conclusion: Since the patients with primary immunodeficiency are prone to multiple infections, various malignancies can be seen in these people. The recurrence of some malignancies in families, especially blood malignancies may be due to primary immunodeficiency and we should consider evaluation of immune system.
Abbas Khalili,
Volume 32, Issue 1 (4-2024)
Abstract
Introduction: Inborn error of immunity (IEI) includes variety of genetic disorders that affect both the innate and acquired immune system. The number of primary immune deficiency disorders is increasing due to advanced laboratory testing methods. The patients with IEI are susceptible to multiple infectious and non-infectious diseases، including autoimmune disorders، allergies، and malignancies. Several studies have shown an increasing prevalence of non-infectious disorders in patients in IEI. Among the non-infectious manifestations of primary immunodeficiency، allergic symptoms can play an effective and important role in diagnosis and treatment of these patients.Articles were searched in Google Scholar، PubMed، Web of Science، and Scopus databases. In this review، we have tried to use the latest and most reliable articles related to the subject that have been published in Persian and English language.
Conclusion: Allergic diseases can be a common part of some primary immunodeficiency diseases. Even in some types of primary immunodeficiency، they are considered the main symptom and important indicator of the disease، which are very decisive factors for the diagnosis of immunodeficiency.
Abbas Khalili, Amir Hossein Karimi,
Volume 32, Issue 6 (9-2024)
Abstract
Introduction: The relationship between viral infections and acute or chronic urticaria is rarely reported in some articles. But its exact mechanisms are not known. There is evidence of urticaria in the patients with covid-19 infection. Therefore, there was no structured research to clarify the role of the corona virus in the pathogenesis of urticaria.
Methods: The articles that were published from January 2020 to October 2022 were reviewed by "PubMed" and "Google scholar" databases using the keywords acute urticarial urticaria, chronic urticaria along with COVID-19. Inclusion criteria were articles reporting acute or chronic urticaria in the patients with confirmed COVID-19 infection in English. Review articles and studies that reported suspected corona infection and in which urticaria were related to drug hypersensitivity and other allergic diseases were considered as exclusion criteria.
Results: In this search, 43 articles were reviewed that were related to our goals. Among these articles, 27 studies that met the inclusion criteria were selected. 16 articles were case reports, case series, letters to the editor and 7 original articles. In this review, it was reported 17 patients in 16 case reports and case series with urticaria associated with COVID-19.
Conclusion: The severity of chronic urticaria may increase with corona infection. Acute urticaria may be a precursor symptom of COVID-19 infection. Finally, due to the small number of articles and some contradictory results, more studies are suggested in this field.
