Showing 5 results for amoabediny
A Basiri, Gh Amoabediny, M Vasei, M Soleimani,
Volume 22, Issue 3 (Jul- Aug 2014)
Abstract
Introduction: Poly lactic co- glycolic acid (PLGA) and poly caprolacton (PCL) are highly applicable polymers in the field of drug delivery and tissue engineering scaffolds. Therefore, this study aimed to design an insulin-loaded PCL/PLGA hybrid nanofiber scaffold in order to be applied in attachment and growth of chondrocytes. Moreover, it can provide a vehicle for the controlled release of active insulin in a certain time period.
Methods: Chondrocyte cells were isolated from septum cartilage tissue utilizing collagenase and were also cultured in monolayer, then the third-passage cells were seeded on the scaffolds. Insulin release from the PCL/PLGA hybrid nanofiber scaffold was examined by Radio Immuno Metric Assay (RIMA) during 22 days. Adherence, distribution and morphology of cells were observed by H&E and alcian blue staining.
Results: PCL/PLGA hybrid nanofiber scaffold revealed a slow and sustain release of insulin within three weeks. Chondrocytes were distributed evenly throughout the scaffolds. In addition, they sank into the pores of scaffold and maintained their rounded morphology.
Conclusion: Biological activity of insulin has been maintained during 22 days of controlled release from hybrid nanofiber PCL/PLGA scaffold. Chondrocytes were distributed evenly throughout the scaffold and revealed a rounded morphology. Therefore, this scaffold provides a suitable carrier for chondrocyte growth as well as formation of tissue engineered cartilage.
Mm Ochi Ardebili, Gh Amoabediny, Sm Rezayat, A Akbarzadeh, B Ebrahimi,
Volume 23, Issue 3 (May-Jun 2015)
Abstract
Introduction: Nano-liposomes are Nano particulate vesicles with lipoid membrane which are under extensive investigation as drug carriers for improving the delivery of therapeutic agents. This study intended to enhance efficacy of Silibinin herbal drug in Nano liposome system (Nano phytosome) via encapsulation for delivery to liver cancer cells. Silibinin is one of the anti-cancer drugs, which its antitumor efficacy is primarily attributed to decreasing N-nitrosodiethylamine in hepatocit carcinoma cells. Nano Liposome encapsulation of Silibinin can dramatically improve its biological activity and increase stability of Silibinin in blood.
Methods: Small uni-lamellar (SUV) vesicles entrapping Silibinin were prepared using DiPalmitoyl PhosphatidylCholine (DPPC), cholesterol: DSPE-MPEG2000 at 7:4:0.36 molar ratio , the fluorescent label (DIL) incorporated in the lipid bilayer at 0.09 mol % as lipophilic phase and buffer of HEPES as hydrophilic phase. Moreover, Nano Liposome size, Zeta-potential, encapsulation efficiency and release of drug were determined after Nano Liposome production .
Results: The study results demonstrated that mean nano-liposome diameter was 46.3 nm. The size and structure of Nano-liposomes were analyzed by SEM and TEM images. The zeta potential of the encapsulated Nano-liposomes was shown -23.25. The encapsulation efficiency for Silibinin was about 24.37%.
Conclusion: In this study, silibinin drug encapsulated nano-liposome controlled release system to improve the solubility and bioavailability of silibinin for delivery to liver cancer cells.
Fa Haghiralsadat, Gh Amoabediny, Mh Sheikhha, J Mohammad Nezhad, S Naderinezhad, Z Malaei-Balasi, B Zandieh Doulabi,
Volume 24, Issue 10 (Jan 2017)
Abstract
Introduction: Cancer is one of the most harmful disease throughout the world. Doxorubicin is an anti-cancer agent, used in the treatment of various types of the cancer such as bone cancer. There are several adverse effects related to clinical usage of Doxorubicin for long time. The present study aimed to investigate the reducing side effects and enhancing the therapeutic effect by liposomal carrier.
Methods: Liposomes containing DPPG and cholesterol with the molar ratio of 70:30 with the Doxorubicin were synthesized by pH- gradient method. The average diameter of nanoparticles and surface charge was determined by Zeta-Sizer instrument. The amount of drug loaded and drug-released was determined using dialysis. The surface morphology and internal lamella was evaluated by TEM and SEM.
Results: The average size of liposomal Doxorubicin obtained using Zeta-Sizer was 126 nm. The encapsulation efficacy of liposomal Doxorubicin was 89%. The total amount of drug release during 48 hours in acidic medium studied by dialysis technique was 46%.
Conclusion: In this study, investigation of loading Doxorubicin into nano-liposome with the slow- released kinetic was carried out to improve the solubility and bioavailability of Doxorubicin in order to delivery to osteosarcoma cell line.
Z Malaei-Balasi, Gh Amoabediny, D Biria, F Atyabi, N Salehi-Nik, F Haghirosadat,
Volume 25, Issue 2 (May 2017)
Abstract
Introduction: The successful application of nanoliposoms as an effective drug delivery system depends on their stability in the medium. In this article, influence of additive materials such as cholesterol and sucrose besides two natural and synthesized phospholipids have been investigated.
Methods: In the present study, designing and synthesis of nanoliposomal formulations were prepared using thin film method. This liposomal suspension was downsized by two methods, the high-pressure homogenizer and ultrasound to form small unilamellar vesicles. The size distributions, zeta potentials and phase transition temperature of formulations were all determined by a zetasizer and differential scanning calorimetry(DSC). In addition, the contribution of nanoliposomal formulation has been investigated by HPLC and FTIR methods.
Results: Results of the DSC measurments indicated that incorporation of unsaturated phospholipid (SOY PC) may cause phase separation with partial miscibility in the liposome bilayer containing of DPPG. The optimal nanoliposomal formulation was composed of (DPPC: CHOL: mPEG2000-DSPE) with the mole percents equal to (83:15:2), respectively. In addition, sucrose has been used in the formulation with a total amounts six times greater than that of the lipids. The properties of optimized nanoliposome have been shown as the size average 104nm, zeta potential 8.04mv and phase transition temperature of lipid less than 37°C which were stable enough to be utilized for loading and releasing bioactives in body temperature.
Conclusion: Finally the produced nanoliposomes were stable vesicles in the proper size, phase transition temperature and surface charge without any aggregation and fusion.
Bibi Fatemeh Haghiralsadat, Ghasem Amoabediny, Samira Naderinezhad, Farzane Morteza-Ratki, Behrouz Zandieh Doulabi,
Volume 26, Issue 12 (Mar 2018)
Abstract
Introdution: Cationic liposomes have been presented for gene delivery as an alternative vector instead of viral vectors. A major challenge associated with siRNA delivery is the instability of liposomes, which is still a serious problem. The aim of this study was to provide an appropriate formulation to overcome this instability.
Methods: In the present study (Scientific-Fundamental, Experimental-Laboratory Study), liposomal formulation containing soy phosphatidylcholine, cationic DOTAP, cholesterol and polyethylene glycole was synthesized by thin-film hydration method and the siRNA were loaded on liposomes through incubation. In the following; the optimization of siRNA loading was on the agenda. Then the parameters related to size, zeta potential, polydispersity index and lon-term stability of siRNA-liposomes complex were reported. The Data were analyzed by GraphPad Prism version 7 Software. All data were repeated three times and reported as mean±standard deviation.
Results: In this study we were able to produce siRNA lipoplex with high loading efficiency of siRNA. The produced nanoparticles did not agglomerate and were stable at 4 oC for 3 months. This nanosystem could successfully deliver siRNA to normal bone cells. Studies have shown that the blank system (no gene) had no toxicity.
Conclusion: The prepared PEGylated liposomes have a great potential for delivery of siRNA to bone cells
