Showing 18 results for Tehrani
B Tehrani Neshat, M Azizzadeh Fooroozi, S Mohammad Alizadeh,
Volume 12, Issue 4 (winter 2005)
Abstract
Introduction: Heparin is only fast acting anticoagulant drug available in the injectable form. Skin discoloration is an adverse and unpleasant outcome of subcutaneous heparin injection that is observed frequently and causes the patient both physical and psychological discomfort thus limiting the further sites for injection. It is therefore important that nurses use an injection technique that minimizes the incidence of adverse outcomes. This research is a quasi-experimental study carried out to determine the relation between duration of injection of subcutaneous heparin and extent of local skin discoloration at the Fatima and Shahid Beheshti cardiac hospitals, Shiraz, 2002 .
Methods: 167 patients were selected and sampling was done by selecting consecutive samples. Data collection was done by using a researcher-made check-list consisting of section of demographic characteristics and a section to record the extent of discoloration at 48 and 60 hours after injection. Data analysis was done by distributional index and parametric (paired-t test) and Bi-parametric (Mann witney-U and kruskal wallis).
Results: Extent of local skin discoloration was less in 30-second injection technique, so that the mean size at 48 and 60 hours after 10-second injection techniques (82.85 214.3,77.96 206 ,respectively) was more than the mean size at 48 and 60 hours after 30-second injection technique (40.53 148.11,44.41 175.51 respectively).There was a significant relationship between sex and size of discoloration (p<0.001),so that mean size in females in the 10-second and 30-second injection techniques at 48 and 60 hours after injection was more than that in males.
Conclusion: On the basis of results of this research, administering subcutaneous heparin injection over longer duration, especially in females reduces injection site discoloration.
Mm Akhoundi, E Tork-Abadi, Aa Bayat, M Heidari, R Ghods, Mr Sadeghi, A Dizeji, F Shokri, M Jedi-Tehrani,
Volume 13, Issue 4 (Atumn 2005)
Abstract
Introduction: As monoclonal antibodies are potential tools for characterization of soluble or cellular surface
antigens, use of these proteins has always been considered in infertility and reproduction research. Therefore,
in this study, monoclonal antibodies against human sperm surface antigens were produced.
Material and Methods: To produce specific clones against human sperm surface antigens, proteins were
extracted using solubilization methods. Balb/c mice were immunized intraperitoneally with the proteins
using complete Freund’s adjuvant in the first injection and incomplete Adjuvant in the following booster
injections. Hybridoma cells producing ASA were cloned by limiting dilution.
Results: Five stable ASA producing hybridoma clones were achieved and their antibody isotypes were
determined by ELISA. All the isotypes were of IgG class. Their cross reactivity with rat and mice
spermatozoa was examined but they did not have any cross reactivity.
Conclusion: The produced antibodies can be used in further studies to characterize and evaluate each of the
antigens present on human sperm surface and determining their role in fertilization.
M Tehranipour, Z Javadmoosavi,
Volume 19, Issue 3 (Jul-Aug 2011 2011)
Abstract
Introduction: Injuries of the peripheral nerve system affect the neurons cell body leading to axon injury. Cannabis sativa plant has anti oxidant and anti apoptotic effects. Therefore the aim of present study was to study the neuroprotective effect of alcoholic extract of cannabis sativa leaves on neuronal density of alpha motoneurons in spinal cord after sciatic nerve injury in rats.
Methods: In this experimental research, animals were divided into four groups A: control, B: compression, C: compression+ treatment with 25 mg/kg alcoholic extract, D: compression + treatment with 50 mg/kg extract (n=8). At first, sciatic nerve compression in B, C and D groups was achieved for 60 seconds using locker pincers. Alcoholic extract was injected intra peritoneally in the first and second weeks after compression. Then 28 days after compression, under profusion method, the lumbar spinal cord was sampled and the numerical density in each group was compared with the compression group. The data was analyzed with the use of Minitab 14 software and ANOVA statistical test.
Results: Neuronal density showed a meaningful difference in the compression and control groups(P<0.001). Neuronal density in treatment groups(25, 50 mg/kg) also had a meaningful increase(P<0.001) as compared to the compression group.
Conclusion: Alcoholic extract of cannabis sativa leaves has a neuroprotective effect on spinal cord alpha motoneurons after injury. This could be due to growth and regeneration factors present in the alcoholic extract of cannabis sativa leaves that induce regeneration process in injured neurons or prevent degeneration.
M Tehranipur, Z Javadmoosavi, S Mohammadi,
Volume 20, Issue 3 (Jul-Aug 2012)
Abstract
Introduction: Diabetes mellitus is a metabolic disorder with systemic complications. Children of diabetic mothers are at risk of many disorders such as major and minor vascular injuries, retinopathy, and atherosclerosis. The present study aimed to investigate the effects of maternal diabetes on changes in choroid plexus volume and capillaries length in 15.5 day foetus.
Methods: in this experimental study which was conducted on 30 male wistar rats, animals were divided into two groups an experimental and a control group (n=10). Streptotosin was injected (55 mg/kg) in experimental group to induce diabetes while in control group intra-peritoneal injection of buffer citrate was done for four weeks. In the last day of the fourth week, mating was done and zero day of pregnancy was determined with vaginal plug observation. on 15th day of pregnancy, rats were anatomized and a foetus was chosen from each mother. After foetal head fixation and tissue processing, serial horizontal sections were prepared and stained with haematoxylin-eosin. Choroid plexus volume was measured by stereological techniques and the results were analysed using ANOVA statistical test.
Results: Results showed a significant difference between Choroid plexus volume and capillaries length in experimental and control groups, so that Choroid plexus volume and capillaries length were increased significantly in foetus of diabetic mothers(P<0.05).
Conclusion: It is likely that maternal hyper-glycaemia induces a kind of angiogenesis in foetus that can cause many brain disorders.
M Tehranipour, S Samare- Mousavi ,
Volume 21, Issue 2 (May-Jun 2013)
Abstract
Introduction: Axonal injury is followed by choromatolises in cell body of neurons. Peripheral nervous system damages enter to the cell body of alpha neurons retrogradely and cause spinal degeneration. Therefore, in this study, the Solanum nigrum L extract was utilized in living bodies. Since extracted lipids from the leaves of this plant has analgesic, antipyretic and anti-inflammatory effects, this study aimed to investigate the neuroprotective effect of Solanum nigrum L ethyl acetate fraction on alpha motoneurons degeneration rate after sciatic nerve injury in rats.
Methods: 48 wistar rats with average body weight of 250 to 300 gr were divided randomly to six groups (control, compression, compression+ extract 25, 50, 75, 100 mg/kg). In the treated group, in addition to compression, extract was injected intraperitoneally too (2time, every week, I.P). After four weeks, lumbar segments of spinal cord L2-L4 were removed, processed, serially sectioned and were stained with Toluidine blue. The alpha motoneurons were counted by dissector method.
Results: Totally, neuron density of the control group, compression group and 25 , 50, 75 and 100 treatment groups involve 1739, 781, 1189, 1418, 1630 and 1030, respectively. The results show a significant reduction for neuron density in compression group in comparison with the control group, (p <0/05). In all treatment groups, neuronal density has a significant increase compared to the compression group, but this was not observed for100mg/Kg group, (p <0/05).
Conclusion: Results showed that ethyl acetate fraction with dose of 75 mg/kg had the most neuroprotective effect on anterior horn motoneurons of spinal cord after sciatic nerve injury in rats which indicate the antioxidant and anti inflammation activity of Solanum nigrum.
B Tirgary, M Tofighi, M Azizzadeh Foroozy, S Iranmanesh, H Tehrani,
Volume 22, Issue 1 (Mar-Apr 2014)
Abstract
Introduction: Multiple Sclerosis is the most common non - traumatic illness of central nervous system. Due to debilitating nature of the disorder, and its unpredictable progress, there is a great deal of uncertainty about the future health and well-being of MS sufferers. Therefore, this study aimed to assess the perceived uncertainty in patients with MS.
Methods: This is a cross-sectional descriptive study. The study population consisted of 200 non- hospitalized patients With Multiple Sclerosis. Convenience sampling was applied in this study and data was collected via a questionnaire including background information and MUIS-Community questionnaire consisting of 23 items about uncertainty. Data analysis was performed by T-test and ANOVA test.
Results: Results showed that the most active patients (5/79%) were females and 5/67% were married. Overall mean score of uncertainty scale was 67.20 (12/35). The results related to uncertainty dimensions revealed that mean score of ambiguity was 32.24, mean score of complexity was 11.80, mean score of inconsistency was 4.79 and unpredictability was 2.78. The results related to relationship between perceived uncertainty and demographic factors show significant difference between the education level and residence with perceived uncertainty and ambiguity.
Conclusions: Considering that MS patients experience the high levels of uncertainty that it will lead to depression and stress, therefore, with the emphasis on rigorous assessment of patient health and holding educational classes of uncertainty management especially for people with lower education level can be an effective step to reduce stress and depression in patients with Multiple Sclerosis.
Mrs M Alikhanzade, Dr M Tehranipour, Dr J Khayyatzade,
Volume 22, Issue 4 (Sep-Oct 2014)
Abstract
Introduction: The effects of injuries in peripheral nervous system returns to the cell body of neurons in central nervous system in a retrograde manner which leads to brain and spinal degeneration. It is probable that Achillea biebersteinii might prevent the injury progress. Hence, the present study aimed to investigate the neuroprotective effect of alcholic extract of Achillea biebersteinii leaves on spinal α motoneurons degenarion after sciatic nerve compression in rats.
Methods: 30 male wistar rats were divided randomly into groups of control, compression, compression with treatment of 50, 75, 100 mg/kg doses. Sciatic nerve was exposed to compression for 60 s using locker pincers. Extract injection was done intraperitoneally in the first and second weeks after compression. Then 28 days after compression under perfusion method, the lumber spinal cord was sampled. After cutting and coloring, the density of motoneurons was measured using dissector methods.
Results: The study results demonstrated a meaningful difference between compression and control groups in regard with neuronal density (P=0.000). Neuronal density showed also a significant meaningful increase (P<0.01) in alcoholic extract treatment groups of 50,75,100 mg/kg compared to the compression group.
Conclusion: The study findings revealed that Alcohlic extracts of Achillea biebersteinii leaves, containing growth and regeneration factors, has neuroprotective effect on spinal cord alpha motoneurons after injury. The extract with 75mg/kg dose was reported as the most effective one.
M Ferdosi Makan, J Khayatzadeh, M Tehranipour, M Behnam Rasouli,
Volume 22, Issue 6 (Jan-Feb 2015)
Abstract
Introduction: Sciatic nerve compression is one of the factors that may cause the cell body degeneration of the alpha-motoneurons of spinal cord ventral horn. Nigella sativa belongs to the family of Ranunculaceae which owns numerous pharmacological properties. Therefore, this study was conducted to determine the neuro-protective effects of n-butanol and ethyl acetate fractions of Nigella sativa on motoneurons’ density of Spinal Cord Ventral Horn in Rats undergoing compressived injury of sciatic nerve.
Methods: In this study, 24 Wistar male rats with average body weight of 250gr to 300gr were divided into four groups of six: control, compression, A(compression + n-butanol fraction 75mg/kg) and B(compression+ethyl acetate fraction75mg/kg). In compression and treatment groups, sciatic nerve of the right leg underwent compression (30sec). In fact, the extract was injected intraperitoneally twice after the compression. After 28days, lumbar segments of spinal cord L2-L4 were sampled under perfusion method. After going through tissue processes, they were cut in serial sections (7µ), and stained with toluidine blue. Then, the density of alpha-motoneurons of spinal cord ventral horn was measured by using dissector method.
Conclusion: The study findings revealed that n-butanol fraction of Nigella sativa caused an increase in neuronal density which posesses neuroprotective effects. This could be due to antioxidant and anti inflammatory effects of this herb. However, increases in neuronal density in ethyl acetate fraction didn’t prove to be significant.
N Jamshidian Tehrani, H Hadi Nedoushan, Sa Mirghanizadeh Bafghi, Ar Karimollah, M Vakili, M Asadi,
Volume 23, Issue 6 (Aug-Sep 2015)
Abstract
Introduction: Echinops ilicifolius, Echinops jesdianus, Echinops ceratophorus and Echinops lasiolepis are defined as native plants of Yazd that their immunomedulatory effects have not been studied yet. The aim of this study was to determine the effect of different concentrations of these plants on peripheral blood mononuclear cells(PBMCs) proliferation and interleukin(IL)-4 secretions.
methods: Root extracts of Echinops ilicifolius, Echinops jesdianus, Echinops ceratophorus and Echinops lasiolepis were prepared by Maceration method. PBMCs were obtained from three healthy volunteer individuals and cultured with the presence of 0.1,1,10,100 and 200 µg/ml with concentrations of 10 µg/ml of phytohemagglutinin. The rate of cell proliferation was determined by BrdU kit. The IL-4 levels in PBMCs. supernatant were measured by enzyme-linked immunosorbent assay(ELISA). P value<0.05 was considered significant.
Results: The different concentrations of root extracts of all plants showed inhibitory effect on PBMCs. There was a significant difference among Echinops lasiolepis extracts in different concentrations(p=0.045). The levels of IL-4 were similar in super natant in control group and different concentrations and the control groups.
Conclusions: The results showed that root extracts of Echinops species had inhibitory effect on PBMCs proliferation and in some species with decrease in IL-4 secretion might have immunomedulatory effects. The effect of Echinops extract fractions on PBMC is suggested.
F Attarian, S Zafar Bala Nezhad, M Tehrani Pour,
Volume 23, Issue 11 (Feb 2016)
Abstract
Introduction: Angiogenesis is regarded as a complex activity required for physiological process as well as many pathological conditions as solid tumor progression and metastasis. Stachys Lavandulifolia of Laminacea family prossesses antioxidant and anti-apoptosis effects. Therefore, this study aimed to investigate the effect of Stachys Lavandulifolia alcoholic extract on angiogenesis in chorioallantoic membrane of Ross chicken embryo.
Methods: In this experimental study, 30 Ross fertilized eggs were divided into 5 random groups consisting of: control, sham-exposed, treatment with 25, 50, 100 mg/kg doses of alcoholic extract. On the 2nd day of incubation, windows were opened for eggs under the sterile conditions. On the 8th day, a gelatine sponge with 1×4×4 diameter (Albumen and Agar solution in normal saline) was placed on chorioalantoic membrane (CAM) to which extract was added. On the 12th day, length and weight as well as chorioallantoic membrane (CAM) of embryos were photographed. Then, the number and length of vessels in a special area on the CAM were measured by Image J. The study results were analyzed by Minitab (16) via applying t-test and ANOVA.
Results: Length and weight mean of embryos as well as number and length of vessels in control and
sham-exposed groups did not show any significant differences. Length and weight mean of embryos and vessels length in all treatment groups demonstrated a significant difference compared to the sham group, Moreover, mean of vessel number did not reveal any significant differences in dose of 50.
Conclusion: As the study findings indicated, the effect of this extract is dose dependet, so as it demonstrates pro angiogenesis in low doses and anti-angiogenesis effect in high doses.
Ar Ajami , M Tehranipour , Kh Nezhad Shahrokh Abadi , M Zokaee ,
Volume 24, Issue 8 (Nov 2016)
Abstract
Introduction: The expression of neurotrophic factors, which cause increasing of the survival and regeneration of neurons, is changed in response to nerve injury. Cantharellus cibarius has antioxidant and anti-inflammatory effects. So, the aim of present study was to determine the effects of hydro-alcoholic extract of Cantharellus cibarius on expression level of NT3 gene after sciatic nerve compression in rats.
Methods: First the hydro-alcoholic extract of Cantharellus cibarius was prepared by the Soxhlet method. In this study, 36 Wistar male rats , 250-300 gr, were randomly divided into 3 groups consisted of 12 rats in each group. They were control, compression (1, 7, 14 and 28 days) and experimental (1, 7, 14 and 28 days) groups. Experimental groups were treated by 75 mg / kg of hydro-alcoholic extract of Cantharellus cibarius and to induce the stress in control group, saline serum was injected. In compression and experimental groups, the sciatic nerve of right leg was compressed for 60 seconds. The first injection of extract in the experimental group was performed intraperitoneally and immediately after the compression and the second one was injected 7 days later. Then, the sampling was performed of lumbar spinal cord on 1, 7, 14 and 28 days in compression and experimental groups and the total RNA was extracted from the spinal cord segments, cDNA was synthesized and after that the alteration of gene expression of NT3 and NGF samples was studied real time PCR method and Data were analyzed by Tukey test and SPSS 16 softwere.
Results: The expression of NT3 and NGF showed significant increase in compression group compared to the control group, (p<0.05). Also, it is shown significant increase of expression of NT3 and NGF in the experimental group compared to the compression group, (p<0.05).
Conclusion: According to these findings, hydro alcoholic extract of Cantharellus cibarius has an anti oxidant and anti-inflammatory effects that increase the regeneration process expression of NT3 and NGF in the experimental group was increase compared with the compression group.
N Haji Tehrani, F Daryanoosh, Ma Babaee Beygi, Ar Rasekhi, M Koushki Jahromi, M Mohazzab,
Volume 25, Issue 2 (May 2017)
Abstract
Introduction: According to many studies, hypertension is considered as the second leading risk factor for coronary artery disease (CAD) and cardiac infarction after smoking The purpose of the present study was to investigate the effect of four weeks of Isometric Handgrip (IHG) Training on peripheral resistance and hypertension among hypertensive women.
Methods: 14 middle aged women, aged 40 to 55 years old, were randomly selected. The training program included 4 sets of 2 minute IHG training with 1 minute resting between each period, performed 3 days a week for four weeks. Doppler ultrasound method was used to measure the vascular resistance. Data were analyzed using paired t-test.
Results: According to the results, there was a significant difference between systolic blood pressure (p=0.006) and diastolic blood pressure (p=0.001) after 4 weeks of training. However, no significant difference was observed in vascular resistance (p=0.66).
Conclusion: These results demonstrate that IHG training can reduce blood pressure in the hypertensive patients 24 hours after the last training session. It is also revealed that reduced blood pressure is not always associated with reduced vascular resistance and other influential factors may play a role in this regard.
Fateme Niazi, Khadije Shahrokh Abadi, Maryam Tehranipour,
Volume 25, Issue 8 (Oct 2017)
Abstract
Introduction: Angiogenesis is one of the most important biological processes that any disruption of it can cause diseases. The main point of the molecular guidance in this process is the vascular endothelial growth factor (VEGF) that acts through its receptors and is one of the angiogenesis specific regulators. This study aimed to investigate the effect of Ocimumbasilicum aqueous and alcoholic extract on VEGF gene expression changes in chick chorioallantoic membrane.
Methods: In this empirical study, 60 Ross fertilized eggs were randomly divided into 6 groups, including control group, sham-exposed group, and 4 experimental groups. On the second day of incubation, a window was opened on the eggs and on the eighth day 50 and 150 mg/kg of basil aqueous and alcoholic extracts were injected into the chick chorioallantoic membrane (CAM). On the twelfth day, for RNA extraction and VEGF gene expression examination the chorioallantoic membrane was sampled, then cDNA was synthesized and VEGF gene expression changes were studied using quantitative data analysis in all groups.
Results: The results showed that VEGF gene expression- under the influence of aqueous and alcoholic extracts of basil- increased in all experimental groups compared to the sham group, but the rise was more in those groups, which were treated with aqueous extract, in both dosages, than alcoholic extract-treated groups.
Conclusion: Both aqueous and alcoholic extracts of basil increased VEGF gene expression. The study showed an increase in the number and length of vessels. According to the increasing effects, basil aqueous and alcoholic extracts can affect the molecular processes of growth and dependent processes.
Zahra Abasnezhad, Maryam Tehranipour, Saidhe Azafar Balanezhad, Nastaran Amintaheri,
Volume 27, Issue 6 (10-2019)
Abstract
Introduction: Angiogenesis is the formation of new vessels from early vessels that has effected in pathologic condition such as growing tumor, metastases, arthritis rheumatoid, diabetes and physiologic condition such as developmental and growing of organ regeneration, wound healing, and reproduction. Otostegia presica is a member of Laminacea family, has antioxidant and anti-apoptosis properties. The aim of this study was to investigate the effects of hidroalkoholic extract of Otostegia presica on VEGFR expression and angiogenesis in chick chorioallantoic membrane.
Methods: In this research, 60 fertilized Ross eggs were used and randomly were divided into five groups, included: control, sham-exposed, and three experimental groups treated with hidroalkoholic extract of Otostegia presica doses 100,200,400 mg/kg. On 2nd day of incubation, a window was opened for eggs under sterile condition. On 8th day, a gelatin sponge with 1×4×4 diameter was placed on chorioalantoic membrane (CAM) and extract fractions with dose of 100,200,400 mg/kg were added. On 12th day, length and weight of embryos were measured and CAM photos were captured. Then number and length of vessels in special area on CAM were measured with Image J. software. Sampling was performed for assessment of VEGFR expression. Data were analyzed by t-test and ANOVA and p<0.05 was considered significant.
Results: Comparison between mean of length and weight of embryos and number and length of vessels in controls and sham-exposed did not show significant difference (P>0.05). Mean of the number of vessels showed significant increase in all experimental groups (P<0.05). VEGFR expression has increased in experimental groups rather than sham-exposed group.
Conclusion: The results showed that hidroalkoholic extract of Otostegia presica has have angiogenic effects and increased VEGFR expression in all experimental groups
Fariba Ghasemzadeh, Maryam Tehranipour, Khadijeh Nezhad Shahrokhabadi,
Volume 27, Issue 10 (1-2020)
Abstract
Introduction: Neurotrophic factors change in response to nerve damage. Stachyslavandulifolia belongs to the Laminaceae family and since tea has antioxidant and anti-inflammatory effects, therefore, this study aimed to determine the effects of hydroalcoholic extract of mountain tea and its effect on NT3 gene expression after compression.
Methods: In this experimental study, at first the hydro-alcoholic extract of stachys was prepared by the Soxhlet method. In this study, 36 Wistar male rats , 250-300 gr, were randomly divided into 9 groups, 4 rats in each group, and included control, compression (1, 7, 14 and 21 days) and experimental (1, 7, 14 and 28 days) groups. Experimental groups were treated by 75 mg / kg of hydro-alcoholic extract of stachys and to induce the stress in the control group, saline serum was injected. In compression and experimental groups , the sciatic nerve of right leg was compressed for 60 seconds. The first injection of extract in experimental group was performed intraperitoneally and immediately after the compression and the second one was injected 7 days later. Then the sampling was performed of lumbar spinal cord on 1, 7, 14 and 28 days in compression and experimental groups and the total RNA was extracted from the spinal cord segments, cDNA was synthesized and after that the alteration of gene expression of NT3 samples was studied in both samples, without treatment and treated with hydro-alcoholic extract.Data were analyzed using MxPro software and Anova test with a significant level of p <0.05 and Excel software was used for drawing graphs.
Results: The results showed that NT3 gene expression was significantly increased in the compression and treatment groups (p <0.001). Although, the NT3 gene expression was decreased in the treatment group compared to the compression group.
Conclusion: It seems that hydroalcoholic extract of Stachyslavandulifolia shoots did not affect NT3 gene expression.
Hasan Mashroteh, Mohammad Khajeh Mehrizi, Narges Nabizadeh, Nadia Tehrani Dehkordi,
Volume 28, Issue 4 (6-2020)
Abstract
Introduction: In addition to its protective role, dressings not only prevent infection, but also accelerate wound healing process. In this study, the influence of optimizing the properties of the calendered needle-punched nonwoven fabrics using the finishing materials of Honey, Aloe Vera, Chitosan and Nano Argentum Nitrate for the end-usage as dressing has been investigated.
Methods: The prepared samples of the needle-punched nonwoven fabrics were firstly calendered and then, tested for the fundamental expected physical characteristics in the dressing purpose, i.e. crease recovery magnitude, air permeability, etc. in order to distinct initial optimized sample. In the following, the antimicrobial finishing process was carried out on the primary sample by the four studied finishing materials. The finished samples were finally analyzed simultaneously from the antimicrobial aspect and also, for the same physical properties of the dressing, as tested before, to distinguish the ultimate optimized sample. The bacteria of Staphylococcus aureus and Escherichia coli (E.coli) were also employed for antimicrobial tests.
Results: The results demonstrated that combination of four finishing materials of Nano Silver 1%, Aloe-Vera 20%, Honey 7% and Chitosan 1% improved desired properties for wound dressing application in comparison to the other samples from one side and, showed no cellular toxicity and allergy from the other side. The FTIR’s results also implied stretching bonding of hydroxyl group in 3200-3400 wavenumber.
Conclusion: The needle-punched nonwoven fabric finished by the recommended antimicrobial materials can be considered as wound dressing, regarding to sufficient physical properties and hygienic condition.
Maryam Tehranipour, Rahele Pakjame , Saiede Zafar Balanezhad ,
Volume 28, Issue 6 (8-2020)
Abstract
Introduction: The number of neurons in the hippocampus can have a direct effect on its function. The plant Brazembel from the mint family may have antioxidant properties. The aim of the present study was to investigate the effects of stress and Brazmble extract on neuronal density of CA1, CA2 in male rats.
Methods: In this experimental study, Wistar rats were divided into 6 groups (n=5), including control, and negative control under stress, also experimental group,including stress and get Brazmbl doses of the extract mL 50, mL 75 and mL 100. Limiting stress for 21 days, the rats were exposed in 2 hours. Groups receiving the extract for 21 days, to get inside limiting extracts were gavaged. After removal of the brain by perfusion, the hippocampus was excised and stained, and the neuronal density was calculated by dissector and metasteriology. The results were analyzed by Minitab 16 software with t-test and ANOVA at a significant level (P <0.05).
Results: Statistical analysis of the data showed that the mean neuronal density in the stress group compared to the control in CA1 and CA2 had a significant decrease (p <0.01). The mean neuronal density of CA1 region between the stress group (29 ± 3) and hydroalcoholic treatment at 50 mg / kg (25 ± 3) showed a significant increase. In addition, the mean neuronal density in the stress group with 75 mg / kg extract (27±3) and in the group treated with hydroalcoholic extract was 100 mg / kg (25 ± 3). Mean neuronal density of CA2 region between the stress (21 ± 3) and hydroalcoholic treatment at 50 mg / kg (28 ± 3) groups showed a significant increase (p <0.01). The mean neuronal density in the stress group with 75 mg / kg extract was 27 ± 3 and in the hydroalcoholic extract treatment group was 100 mg / kg (22 ± 3), which increased significantly compared to the stress group (p <0.01).
Conclusion: The results of the current study show that the hydroalcoholic extract of plant Bromzebel probably increases the neuronal density of the brain hippocampus due to its polyphenolic substances such as flavonoids, which is due to its high antioxidant properties.
Elham Naghshineh, Hatav Ghasemi Tehrani, Behnaz Khani, Nasrin Bagheri , Najmeh Tavakol, Fatemeh Maghool,
Volume 30, Issue 3 (6-2022)
Abstract
Introduction: Intrauterine insemination (IUI) is one of the assisted reproductive techniques, which increases the likelihood of egg being exposed to sperm in the uterus and thus the chances of pregnancy. The purpose of the present study was to compare the efficiency of the Masstec catheter (the internal product) with imported catheter (PMIU) so that, if similar, use domestic production as an alternative to imported.
Methods: This study was a randomized controlled clinical trial continuous sampling method performed in Shahid Beheshti Hospital in Isfahan between 2016-2017. In this study, out of 62 infertile couples, 30 used catheters (Masstec), produced by Salamat Yar Hakim Engineering Company - Iran, and 32 imported catheters (PMIU), produced by Ellios BioTek – France. Primary outcomes included difficulty in catheter placement, the need to use a tenaculum, and the need for cervical dilatation during IUI. Secondary outcomes included chemical pregnancy, clinical pregnancy, and abortion. The data were analyzed using Chi-square, t-test and logistic regression.
Results: Fisher exact test results showed no significant difference in primary outcome between the two groups (P>0.05). Secondary outcomes, including chemical, clinical and abortion rates were not significantly different between the two groups (P>0.05). Efficacy and side effects of Masstec IUI catheter were not different from PMIU catheter.
Conclusion: Our study showed that the efficacy and side effects of the Masstec IUI catheter were not different from PMIU catheter. Given the similar efficacy and side effects and lower cost of the IUI catheter produced by the local company than the imported catheter, the use of local catheter is recommended for IUI candidate patients.
