Showing 9 results for Tabandeh
M Kolahi , Mr Tabandeh , S Saremy , Sa Hosseini , M Hashemitabar ,
Volume 24, Issue 3 (Jun 2016)
Abstract
Introduction: Polyphenolic compounds have anti proliferative and induced apoptotic features on cancer cells. p-Coumaric acid can be abundantly found in fruits, vegetables, plant production and honey. . Breast cancer is the most frequently diagnosed cancer among women in the world. This study aimed to investigate the effect and mechanism of p- coumaric acid on apoptosis of MCF-7 breast cancer cells.
Methods: In order to study appoptic effect of p- coumaric acid, MCF-7 breast cancer cells were treated with different concentrations of p- coumaric acid (10, 37, 70, 150 and 300 mM) for 24 h. Cell viability was determined using MTT assay. Apoptosis markers including phosphatidylserine exposure at the outer leaflet of the plasma membrane were measured using flow cytometery for Annexin V affinity.
Results: Cell viability of MCF-7 cells was decreased with increasing of p- coumaric acid concentration. Maximal effect of p- coumaric acid was observed in cells that treated with 300 mM for 24h (p< 0.05). Viability assay showed that the IC50 of p- coumaric acid in MCF-7 cells was about 40 mM. p- coumaric acid at dose of 300 mM significantly increased the late apoptotic cells with Annexin V+ and propium iodide (PI+) features after 24 h treatment.
Conclusion: The results of this study showed that p- coumaric acid had effective appoptic activity against MCF-7 cells. The results can be helpful in understanding the anticancer mechanism of p- coumaric acid and using it was suggested as an alternative or complementary drug in cancer chemotherapy.
N Erfani Majd, A Shahriari, Mr Tabandeh, Z Soleymani,
Volume 24, Issue 9 (Dec 2016)
Abstract
Introduction: Type 2 diabete is a kind of metabolic disease that it is associated with hyperglycemia, hyperlipidemia and disturbed liver function. The aim of the present study was to evaluate the protective effects of Okra Powder on liver damage in high fat diet fed / streptozotocin (HFD-STZ)-induced type 2 diabetic rats.
Methods: In this experimental study, 25 Wistar Albino female rats with an average weight of (200–220 g) were randomly divided into 5 groups: Group I: (control group) rats were fed the standard diet, Group II: healthy rats that received Okra Powder (200 mg/kg) for 4 weeks; Group III (HFD/STZ group): Rats were fed with high-fat diet (HFD) (60% fat) for 4 weeks and then injected low dose of STZ (35 mg/kg), Group IV: Diabetic rats that received Okra Powder (200 mg/kg) for 4 weeks. GroupV: Diabetic rats that received metformin (200 mg/kg) for 4 weeks. At the end of experiment, biochemical parameters were measured. Liver samples were removed and 5-6 µ sections were made and stained by H&E and Sudan black staining.
Results: The results showed that all the biochemical parameters, except HDL-C and serum insulin were increased in diabetic rats, while they were decreased in Okra supplementation group compared to diabetic rats (p<0.05). The liver structure alterations were improved in treated diabetic rats with Okra Powder and metformin.
Conclusion: Our findings confirmed the potential anti-hyperglycemic and hypolipidemic effects of Okra Powder. Thus, it seems it has an important role in the management of type 2 diabete.
Maryam Naghibzadeh, Rohullah Ranjbar, Mohammad Reza Tabandeh, Abdolhamid Habibi,
Volume 26, Issue 12 (Mar 2018)
Abstract
Introduction: Identifying the factors that influence on the uptake of Neurotrophins is an important goal for brain's health and function. There is some evidence that long-term exercise improves brain function. However, the effects of exercise intensities on the brain remain is unclear. Therefore, the purpose of this study was to compare the effects of high intensity interval (HIIT) and continuous training (CT) on neurotrophic factors gene expression in hippocampus of C57BL/6 mice.
Methods: 30 C57BL/6 mice were randomly assigned to the following three groups: control (Con), interval training (IT), and continuous training (CT). The mice in the exercise group Were trained to run on the treadmill 5 sessions for 8 weeks. HIIT group performed protocol at 85-90% of maximal work rate for periods of 2 min alternating with 1 min intervals at 50-60% of maximal work rate. CT group performed a continuous exercise protocol at 70-75% of maximal work rate. The expression of BDNF, GDNF, and NGF genes was measured using the Real Time-PCR method in the hippocampus. For statistical analysis of the data was used from SPSS version 16 and the one-way ANOVA method.
Results: The result showed that HIIT program significantly increased the mRNA levels of BDNF and GDNF in comparison with CT (p<0.05), and mRNA level of NGF significantly increased in both groups while no significant differences were observed in NGF concentrations among the HIIT and CT groups (p>0.05).
Conclusion: Our results showed that HIIT had a more neuroprotective effect by improving the expression of the neurotrophin genes compared to the LICT in the hippocampus.
Saeid Moradi, Abdolhamid Habibi, Mohammad Reza Tabandeh, Saeid Shakerian,
Volume 27, Issue 3 (7-2019)
Abstract
Introdution: Based on researches, increased levels of vascular endothelial growth factor and antioxidant parameters in areas of the brain that are more affected by Parkinson's disease have a potential therapeutic effect. Therefore, the purpose of this study was to compare the effect of 6 weeks continuous and interval aerobic exercises on vascular endothelial growth factor and superoxide dismutase enzyme; Parkinson's rats.
Methods: In this experimental study, 24 male Wistar male rats (12 weeks) with an average weight of 250 to 350 grams were used. Subjects were treated with reserpine to Parkinson's, then divided into 6 groups. Aerobic exercises were performed for 6 weeks. After 48 hours of the last training session, the rats were killed, then the tissue of the hippocampus was separated from other parts of the brain, and vascular endothelial growth factor and superoxide dismutase enzyme were measured using specific kits. The results were analyzed using one way analysis of variance analysis and Bonferroni post hoc test.
Results: The results of this study showed that 6 weeks of continuous and interval aerobic exercise increased vascular endothelial growth factor levels in Parkinson's rats (p = 0.01). There was no significant difference between the two groups of interval exercises compared to the continuous training group (p = 0.87). Also, these exercises did not significantly affect the superoxide dismutase enzymes (p = 0.47).
Conclusion: Based on the findings of this study, it has been shown that 6 weeks of continuous and periodic aerobic exercise increases the vascular endothelial growth factor, but these exercises have not significantly changed the superoxide dismutase enzyme.
Marziyeh Feyzi, Mohammad Reza Tabandeh, Mehrdad Shariati, Mohammad Amin Edalatmanesh,
Volume 27, Issue 7 (12-2019)
Abstract
Introduction: Adiponectin is one of the most important adipose derived hormone that conflicting data are available about serum changes of adiponectin at different ages.The present study was done to determine the age related changes in serum adiponectin and its association with insulin resistance (IR) indices in aging in male rats.
Methods: In this study, serum samples were obtained from male rats at different ages, including 2, 5, 10, 18, 52 and 72 weeks age (n=10 in each age group). Oral glucose tolerance and glucose stimulated insulin secretion tests were measured using determination of glucose concentrations at 15, 30, 45 and 60 min after oral ingestion of glucose (1 mg/kg body weight) for each animal. Serum adiponectin and insulin levels were determined using species specific ELISA kits. HOMA-IR was calculated based on glucose and insulin concentrations. Data were analyzed using SPSS version 16 software (SPSS Inc., Chicago, IL) also using one way analysis of variance and LSD posthoc tests.
Results: Our results showed an age dependent decrease in serum adiponectin concentration, and 72-week old rats had the lowest level of adiponectin compared with those in other ages (p<0.05). IR indices, including fasting glucose, insulin, HOMA-IR and response to oral glucose ingestion was increased in an age dependent manner and 72-week old rats showed the highest levels of the IR indices.
Conclusion: Regarding the role of adiponectin in glucose homeostasis and insulin sensitization, it seems that reduction of serum adiponectin with age progression may be an important mechanism of insulin resistance in aging.
Nabiallah Khoonmirzaie, Mohammad Razi Jalali, Ali Sahriari, Mohammad Reza Tabandeh,
Volume 28, Issue 1 (5-2020)
Abstract
Introduction: The bacterial Asparaginase is used in the treatment of asparagine-dependent tumors, particularly lymphatic sarcoma and acute lymphoblastic leukemia. However, the instability of the enzyme increases the number of injections that are accompanied by high immune responses. The aim of this study was to investigate the conjugation of L-asparaginase with nanochitosan glutaraldehyde (NCG) derivative and its effect on the physichochemical properties of conjugated enzyme.
Methods: In this experimental study, nanochitosan was synthesized using reduction method with acetic acid and its physicochemical properties were evaluated using transmission electron microscopy and particle size analyzer. Activated NCG derivative was produced using 3% acetic acid. The conjugation of NCG derivative to L-asparaginase was performed with different molar ratios of enzyme/nanochitosan (1:2, 1:5, 1:10 and 1:20) in the presence of sodium cyano borohydrate; and the ratio with the highest residual activity was used for physicochemical evaluation. The activity of enzyme at different temperatures and pH, its half-life and stability after freezing and resistance to proteolysis were analyzed through repeated measure analysis of variance using SPSS 18 software.
Results: The results of this study showed that the conjugation of L-asparaginase with NCG derivative resulted in maintaining the 70% enzyme activity. The activity of conjugated enzyme was higher than native enzyme after freezing and trypsin treatment. The optimum pH and temperature of conjugated enzyme did not change, while it had higher activity in wide range of pH and temperature compared with native enzyme.
Conclusion: Conjugation of L-asparaginase with NCG derivative improved physicochemical and stability of enzyme and this method can be used for production of improved L-asparaginase for clinical application.
Mohammad Rami, Mohammad Fathi, Masoud Rahmati, Mohammad Reza Tabandeh,
Volume 28, Issue 2 (6-2020)
Abstract
Introduction: Diabetes is one of the most common metabolic diseases that is associated with high blood sugar and can cause disorders of the central nervous system. The purpose of the present study was to investigate the effect of a course of endurance activity on the levels of hippocampal Pannexin-1 and NLRP-1 proteins in diabetic Wistar rats.
Methods: In this experimental study, 28 rats with 10 weeks of age and average weight of 245±9.4 g were randomly divided into 4 groups of 7: control diabetic, trained diabetic, healthy control and healthy trained. Intraperitoneal injection of streptozotocin (45 mg/kg) was used to induce diabetes. Then, the endurance training protocol was performed for 6 weeks. 24 h after the last training session, hippocampal tissue of rats was extracted and Pannexin-1 and NLRP-1 protein levels were measured by Western Blot Method. In this study, one-way analysis of variance and Tukey test as a follow-up test were used. Statistical analysis was performed using SPSS software version 18.
Results: The results showed that the expression of Pannexin-1 and NLRP-1 protein was significantly increased in diabetic rats (P<0.05). Moreover, the results of Pannexin-1 and NLRP-1 protein expression showed that the expression of both proteins decreased significantly after 6 weeks of endurance training in the training diabetic group compared to the control diabetic group (P <0.05).
Conclusion: Our findings confirmed the effects of hyperglycemia induced by endurance physical activity and consequently its effect on the decreased expression of Pannexin-1 and NLRP-1 proteins. Therefore, it seems that physical activity may play an important role in ameliorating neurodegenerative disorders in type 1 diabetic patients by reducing the expression of these proteins.
Saleheh Shahmoradi, Fatemeh Yazdian, Amin Janghorbani, Leila Satarian, Farnaz Behroozi, Fatemeh Tabandeh, Bibi Fatemeh Haghirosadat,
Volume 29, Issue 9 (12-2021)
Abstract
Introduction: Age-related macular degeneration (AMD) is one of the retina diseases in which retinal pigment epithelium cells are degraded and lead to blindness. Available treatments only slow down the progression of it. In this study, human embryonic stem cells (hESCs) differentiated into retinal pigment epithelium cells were cultured on a polycaprolactone scaffold.
Methods: The optimization of the diameter of the produced scaffolds by electrospinning method was done using the fuzzy method for the first time. To improve cell adhesion and proliferation, related parameters to alkaline hydrolysis method were optimized and hydrophobic surface of scaffold was modified. After in vitro analysis, cells were cultured on different groups of scaffolds. In vivo analyses were done and cells culture on scaffolds observed.
Results: The optimal parameters for the scaffold based on the fuzzy model were 18.1 kV for voltage, 0.07 g / ml for solution concentration and 115 nm for scaffold diameter, respectively. The immersion time of the scaffold in alkaline solution and concentration of solution were measured 97 min and 3.7 M, respectively. The treated scaffold had a higher degradation rate and water adsorption. MTT-Assay results showed that scaffolds with modified surfaces had a higher amount of cell viability and proliferation after 7 days. SEM image results confirmed this finding after almost two months. Additionally, the results of ICC test showed that after passing this time, cells kept their RPE and epithelium.
Conclusion: Based on the results, the hydrolyzed scaffold is a suitable substrate for cell proliferation and can be a good option for AMD treatment.
Maryam Janbozorgi, Abassali Gaini, Siroos Choobineh, Mohamad Reza Tabandeh,
Volume 30, Issue 6 (9-2022)
Abstract
Introduction: Chronic hyperglycemia is associated with an increase in cellular damage due to oxidative stress in pancreatic tissue. The effect of exercise in different phases of the circadian cycle on protecting pancreatic tissue from oxidative stress in diabetic patients is unknown. The aim of this study was to investigate the effect of eight weeks of endurance training in light and dark phases of circadian rhythm on the oxidative stress index in pancreas of diabetic mice.
Methods: In this study, 18 mice from the Naval Medical Research Institute (26±3.22 gr) were selected and after inducing diabetes through high-fat food and Streptozotocin injection (20 mg/kg), randomly divided into 6 groups: light phase healthy control, dark phase healthy control, light phase diabetic control, dark phase diabetic control, light phase diabetic training and dark phase diabetic training. The endurance training protocol (50-60 %Vmax) was performed 5 d/w for 8 weeks. After anesthesia, blood samples and pancreatic tissues were removed. Insulin resistance markers, oxidative stress index and expression of Brain and Muscle ARNT-Like1 protein expression were measured in pancreas of diabetic rats. Data were analyzed by one way analysis of variance at the significance level of p <0.05.
Results: The eight weeks of endurance training significantly decreased insulin resistance markers (p=0.005), oxidative stress index (p ˂0.05) and significantly increased the Bmal1 protein expression (p=0.009). The mean values of all variables showed significant differences between light and dark phases.
Conclusion: Endurance training may improve insulin sensitivity and oxidant damage in diabetic conditions by increasing the function of the antioxidant system and the expression of Circadian regulation proteins. Activity in the dark phase causes further increases the metabolism of cells. As a result, performing these types of exercises in the dark phase is recommended to these patients as a new treatment strategy.
