Showing 2 results for Shokrzadeh
M Shokrzadeh, F Hosseini- Shirazi, S Saeedi Saravi,
Volume 15, Issue 3 (Autumn 2007)
Abstract
Introduction: Cell culture is a process in which the cells ware isolated from original tissue, dispersed in liquid media and then placed in culture plate where the cells adhere together and propagate. Today, this method is used for assessment of cell toxicity, its mechanisms and effect of different compounds on intracellular components.
Methods: Clonogenic assay was used for assessment of cell toxicity and amount of cell death after a specific time during which cells were exposed to different compounds. Thus, IC50 in caner cell lines (HePG2, SKOV3 and A549) and normal cell (LLCPK1, CHO and HGF1) was assessed after exposure to cisplatin, acetaminophen and arsenic.
Results: Results showed that acetaminophen has maximum resistance and minimum sensitivity in CHO line with IC50=16.7±1.06 HePG2 with IC50=18.6±1.29. On the other hand, cisplatin showed minimum resistance and maximum sensitivity in HePG2 with IC50 = 0.87±0.07 and HGF1 with IC50 = 1.6±0.21 and lastly, arsenic showed minimum resistance and maximum sensitivity in A549 with IC50 = 4.59±0.29 and LLCPK1 with IC50= 1±0.37.
Discussion: According to the evaluated IC50, there were differences between results of sensitivity of cell lines exposed to the three drugs (P<0.05). Entirely, resistance in cancer cell lines was lower than normal cells. The results showed the importance of cell defensive mechanisms encountering different substances like glutathione.
Mohammad Shokrzadeh , Amir Hajimohammadi , Azam Delaram , Akram Pouyan Sadr, Yaghoub Shayeste ,
Volume 25, Issue 10 (Jun 2018)
Abstract
Introduction: Acute poisonings in adolescents – a common worldwide problem- is a preventable cause morbidity and mortality. The aim of this study was to determine the causes of acute poisoning in hospitalized adolescents in Gorgan.
Methods: This cross-sectional study was conducted on all adolescents (aged 12-19) patients admitted for acute poisoning who are hospitalized in 5 Azar Hospital in Gorgan, during March 2008 to March 2015. Data were analyzed using SPSS software (version 16) and Pearson's chi-squared test.
Results: A total of 800 poisoning cases, 161 cases were due to adolescent poisonings. The majority of the 161 patients were female (59%). The mean age of them were 16.98 years and the majority of patients (73.9%) lived in urban areas. The maximum number of poisoning occurred during autumn (29.2%) and summer season (28%). 64.5% of cases had been educated up to high school. Intentional poisoning (suicide) were identified as the main kind of poisoning (90.7%). The most common agents involved in acute poisoning were drugs (85.1%), especially benzodiazepines (31.1%), followed by Tramadol (19.3%). Overall, 5 patients (3.1%) died and family quarrel was the main cause of intentional poisoning (64.4%).
Conclusion: Intentional poisoning by pharmaceutical agents especially benzodiazepines and Tramadol were identified as the main cause of poisoning in adolescents in Gorgan. Finally, preventive measures should include life skills training, providing readily available counseling services and restricting the reach of drugs.
