Showing 12 results for Rezvani
M Mohammadi, A Amin-Alroaaya, H Rezvanian, A Kachoie, M Amini,
Volume 13, Issue 3 (Summer 2005)
Abstract
Introduction: Hyperhomocysteinemia is associated with an increased risk of cardiovascular disease in diabetic patients. The aim of this study was to determine plasma homocysteine levels in diabetics and compare with control persons and also evaluation of correlation between plasma homocysteine concentration and diabetic related variables.
Methods: This study was done on 39 type II diabetic patients, 27 subjects with impaired glucose tolerance and 23 normal persons referring to Endocrine and Metabolism Research Center of Medical university of Isfahan. Physical characteristics were recorded. After an overnight fast, a blood sample was drawn for determination of FPG, HbA1c – homocysteine, Cr. Cholesterol – Triglyceride and HDL- Cholesterol. Mean plasma homocysteine levels in the groups were compared by one way ANOVA. The Correlation of different parameters was tested by Pearson’s correlation.
Results: There was no statistically significant difference in the plasma homocysteine levels of all the groups.(P=0.71). Correlation between homocysteine levels and HbA1c was not significant (P=0.42) in diabetic patients.
Conclusions: The findings suggest that diabetes does not influence plasma homocysteine levels. Also, there is no correlation between plasma homocysteine levels and HbA1c in diabetic patients
Gh Hassanshahi-Raviz, Mr Hajizadeh, Mr Mirzaee, Aa Pourshanazari, Ma Rezvani, R Vaziri, A Dickson,
Volume 15, Issue 2 (Summer 2007)
Abstract
Introduction: Chemokines are low molecular weight proteins (8-17kDa) with the main role of immune cells recruitment to injured tissues. IP-10/Mob-1 is a CXC chemokine and different cell systems in response to external stimulation produce this chemokine. Various signaling pathways are used by cell and tissue systems to regulate production of proteins e.g. chemokines. Therefore we have investigated some of these pathways leading to production of IP-10/Mob-1 by primary cultured hepatocytes.
Methods: In the present study, hepatocyts were isolated from male Sprague Dawley rats and cultured on Waymouth medium in presence and absence of different inhibitors such as SB203580, MG132, KN62 and Staurosporine for indicated time points. Supernatant medium of culture was centrifuged and proteins were isolated by SDS-PAGE and transferred to nitrocellulose membranes, then membranes were incubated with IP-10/Mob-1 antibody overnight and followed by incubation with secondary antibody and then IP-10/Mob-1 was detected by ECL.
Results: We showed that the expression of IP-10/Mob-1 has been decreased in presence of Staurosporine (10 and 50μM), SB203580(50μM) MG132 (20μM) and KN62 (10 and 20μM) but did not change in absence or lower concentrations of these inhibitors.
Conclusion: Hence, these in vitro data may aid to a better understanding of the pathways in chronic liver injuries and identify clinical studies that may aid in treatment or prevention of these conditions. Furthermore, there may be further potential to prevent changes to hepatocyte phenotype and allow isolation of hepatocytes with a greater physiological phenotype. Therefore it could be concluded that all of these pathways are used by hepatocytes regarding expression of IP-10/Mob-1.
Mb Owlia, S Rezvani- Nejad, H Soleymani,
Volume 16, Issue 2 (Summer 2008)
Abstract
Introduction: Rheumatoid arthritis (RA) is the most common autoimmune joint disease. Osteoporosis is a well-known consequence of RA. Objective: The aim of the present study was to determine the interrelationship between radiographic findings and bone mineral density (BMD) in patients with RA.
Methods: 70 patients with established RA (according to 1987 ACR criteria) were included in a cross-sectional study. BMD at lumbar vertebrae and hip were measured using DEXA machine. Plain radiographs of both hands of all patients were examined and scored according to the Larsen scoring system.
Results: Sixty-five females (92.9%) and five males (7.1%) enrolled in the study. Mean of T-score in these patients was -1.06 in femoral region and -1.56 in lumbar regions. According to lumbar T-score, 50% of patients who were graded in group C Larson score (grade 4, 5) had osteoporosis and 3.33% of patients in this group had osteopenia. A high radiologic Larson score was associated with low BMD at hip and lumbar regions. Age was positively associated with low BMD.
Conclusion: Higher radiologic Larson score was associated with reduced BMD in our RA patients. Because of high prevalence of osteopenia in group B (grade 2, 3 Larson score), we suggest routine bone densitometry in this group and taking into consideration anti-resorptive therapy. However, more studies are required to establish and confirm the results.
A Davari, A Daneshkazemi, A Rezvani, B Taghinejad,
Volume 19, Issue 6 (Jan-Feb 2012)
Abstract
Introduction: In order to improve caries detection, invasive methods should not be used and caries detector is useful, and antibacterial solutions can be used as well as a biological method for caries detection. Aim of the present study was to differentiate sound dentin from infectious dentin by two coloring solutions: acid red and povidone iodine.
Methods: In this experimental investigation, 140 carious molar teeth were collected. Teeth were kept in normal saline solution in room temperature and were divided into two groups, A and B (70 samples in each group). Teeth in group A were painted by acid red 1% solution and in group B by povidone iodine 10% and the painted points were recorded. After omission of the painted points, all samples of group A were painted by povidone iodine (group C) and all samples of group B by acid red (group D) and the painted points were recorded again. Data was analyzed using chi-square and Mc-Nemar tests.
Results: Diagnostic accuracy of acid red for detection of dental caries was more than povidone iodine and the difference was statistically significant (P< 0.05).
Conclusion: It was concluded that the coloring power and accuracy of acid red is more than povidone iodine.
M Esmailidehaj, Z Khaje Bahabadi, Me Rezvani,
Volume 21, Issue 5 (NOv-Dec 2013)
Abstract
Introduction: Today it has been documented that herbal medicines like synthetic drugs have side effects. The aim of his study was that whether assafoetida in doses used in most of previous studies have any effect on hepatic, renal, cardiac and blood biochemical parameters of rats.
Methods: Forty male Wistar rats, with average weighting of 300 grams, were divided into five groups of eight in each. The first group was considered as control group and groups 2-5 as the pretreatment groups that were pre-treatmented by 12.5, 25, 50 and 100 mg/kg tear assafoetida for 30 days, respectively. Then, blood samples were collected for measurement of biochemical parameters of liver (AST, ALT, ALP), kidney (Urea, Cr), cardiac (CPK) and blood coagulation (Pt, Ptt). Blood pressures were also recorded through inserting a cannula into the carotid artery. Lead II of electrocardiogram was used to monitor electrical activity of the heart. Data was analyzed by one-way analysis of variance.
Results: The plasma level of AST, CPK and Ptt significantly reduced in pretreatment groups with assafoetida (P<0.05). With increasing the dose of assafoetida, plasma urea concentration reduced and plasma Cr concentration and all blood pressures increased insignificantly.
Conclusion: The findings of this study suggest that low doses of tear assafoetida (25 mg/kg) does not probably have any side effects and even maybe useful for health, but at higher doses (100 mg/kg) it may lead to complications. Hence, there is a need for further studies to answer some questions that arose from this study.
V Ehsani, M Allahtavakoli, Mh Dashti, Me Rezvani, H Rezazadeh, A Shamsizadeh, M Mobini, E Hakimi,
Volume 21, Issue 6 (Jan-Feb 2014)
Abstract
Introduction: Hypothermia has neuroprotective effects in permanent or transient models of cerebral artery occlusion. In the current study, neuroprotective effect of general hypothermia in the embolic model of stroke, in which no study has been conducted to date.
Methods: In this experimental study, twenty-four male Wistar rats (250 to 350 g) were divided into three groups as following: sham, Control and hypothermia. Stroke was induced by clot injection into the right middle cerebral artery (MCA). General hypothermia was induced at 6 h after stroke. Neurological deficits were measured at 24 and 48 h after ischemia. Infarction volume and brain edema were determined at the end of study.
Results: General hypothermia significantly decreased infarct volume (P<0.001) and neurological deficits (P<0.001). No significant difference was observed between hypothermia and control group in brain edema.
Conclusion: According to the findings of the present study, general hypothermia at six h after ischemia shows neuroprotection in the embolic model of stroke.
Me Rezvani, A Mirgalili, Mh Dashti-Rahmatabadi, Ar Talebi,
Volume 22, Issue 5 (Nov-Dec 2014)
Abstract
Introduction: Acrylamide is a chemical compound which can be produced while frying carbohydrates in high- temperatures. Since the neurotoxicity of acrylamide was reported in the previous studies, the aim of the present study was to investigate the effects of co-administration of Glycyrrhiza Glabra and Vitamin C on acrylamide-induced weight loss and motor deficits in rats.
Methods: In this experimental study, 40 Wistar male rats aged 28 days were divided in to five groups of 8 rats including Control, Acrylamide, Acrylamide + Vitamin C, Acrylamide + Glycyrrhiza Glabra and Acrylamide +Vitamin C + Glycyrrhiza Glabra. All treatments were administered for 6 weeks. Thereafter, locomotor activity indices were measured using open field apparatus. In order to analyze the study data, One-way ANOVA followed by Tukey's test was used.
Results: The results showed that motor activity was significantly increased in vitamin C + Glycyrrhiza Glabra group compared with Acryl amide group. While treatment with vitamin C or Glycyrrhiza Glabra alone did not cause any significant changes in locomotor activity indices. In addition, rats treated with the combination of vitamin C and Glycyrrhiza Glabra significantly improved weight gain deficiency induced by Acrylamide .
Conclusion: The study findings revealed that the co-administration of vitamin C and Glycyrrhiza Glabra can retard the locomotor dysfunction and improve the weight gain in acrylamide-treated rats.
Mohammad Mahdi Mirjalili, Shiva Faramarzi , Manasour Esmaeilidehaj , Fatemeh Zare Mehrjardi , Mohammad Ebrahim Rezvani , ,
Volume 25, Issue 4 (July 2017)
Abstract
Introduction: Delay in wound healing is one of several complications of type 1 diabetes. It has been reported that the use of various herbal extracts such as Morus nigra increase the wound healing. Since,there are no studies on investigation of the effects of Morus nigra on wound healing in diabetics so this study was conducted to determine wound healing effects of Morus nigra in diabetic rats.
Methods: In this experimental study, the fresh fruits were blended and pressed. Then, the extract was filtered and then was passed through the filter paper then it was dried in shade. Diabetes was induced by injection of single dose of streptozotocin (60 mg/kg). The circular wound (diameter 8 cm) was created on the back of each rat. The wounds were topically treated with 10 or 20 percent of concentration of the extract. Wounds in control group were treated with saline and normal group received no treatment. Wound areas were monitored on days 1, 6 and 11; the repaired tissues were removed for histological evaluations.
Result: Daily and local applications of the extract displayed significant wound healing activity through the reducing wound area and acceleration of wound contraction. Histological findings showed that various indices of tissue repairment such as integrated epithelial, typical granulated and regular collagen layers were mor obvious in the treated wounds.
Conclusion: Topical application of the Morus nigra extract can accelrate wound contraction and improve the histological indices inclue formation of epithelial and granulated layers and increase collagen density in the repaired tissue.
Fatemeh Sanjarani , Mansour Esmaeilidehaj, Fatemeh Zareh-Mehrjardi, Tayebe Eghbali , Mohammad Ebrahim Rezvani,
Volume 25, Issue 12 (Feb 2018)
Abstract
Introduction: Effects of many flavonoids have been studied on memory, learning and improvement of Alzheimer. Many flavonoids are effective in the improvement of Alzheimer. Since no study has been conducted on the effect of salvigenin on memory and learning, our aim was to examine the effect of this flavonoid.
Methods: Intraperitoneal injection of D-galactose at a dose of 120 mg / kg was used for 45 for creation of Alzheimer disease model. In this experimental study, 28 male wistar rats weighing approximately 200-250 grams were divided into 4 groups with 7 members, including: normal group: rats that received no drugs, control group: alzheimer disease model rat, which had been stricken to Alzheimer by intraperitoneal injection of D-galactose at a dose of 120 mg/kg and 1, 2 salvigenin groups: in which each group was the same as the control group. Furthermore, they received daily 10 or 20 mg/kg of salvigenin by gavage. In order to evaluate memory, shuttle box and passive avoidance learning was used 2 and 7 days after learning. To assess the mRNA expression rate of BDNF, the entire RNA of hippocampus was isolated and after synthesis of Complementary DNA (cDNA), real time and PCR were done and relative expression of mRNA was evaluated.
Results: The results showed that daily administration of different doses of salvigenin can slow down Alzheimer's induction. The delay duration in entering the dark compartment in trained rats in the treated group was significantly more than the control group. mRNA expression rate of BDNF in salvigenin receiving groups was more than control group.
Conclusion: Generally it can be concluded that salvigenin can improve the memory caused by Alzheimer and also increase mRNA expression rate of BDNF in Alzheimer's rats.
Mahmud Baghbanian, Hasan Salmanroghani, Mohadese Mofidi Naiini, Mohammadkazem Amirbeigy, Saaideh Rezvaninejad, Mohsen Akhondi, Hassanali Vahedian Ardakani,
Volume 25, Issue 12 (Feb 2018)
Abstract
Introduction: Most common causes of lower gastrointestinal bleeding (LGIB) in young patients are benign perianal lesions, but serious colorectal disease may be underlying etiology in this group. The aim of this study was identification of LGIB etiologies in the patients under 40 years old.
Materials: In this retrospective descriptive study, which has been done during 2012-2013, 333 young patients (less than 40 years old) with lower GIB referred to Endoscopy Ward of Yazd Shahid Sadoughi Hospital assessed by colonoscopy with or without histopathology.
Resultus: In this study, 57% of patients were males. Majority of them were between 30-40 years old. The most common presentation was hematochezia (49%) and the most common cause was internal hemorrhoid (17%). Other causes included: inflammatory bowel disease (15%), polyp (9%), infectious colitis (8%), anal fissure (6%), colon cancer (4%), solitary rectal ulcer (4%), vascular lesion (2%), aphtus lesion (1.1%), diverticulosis (0.6%) ,anal tuberculosis (0.3%) and normal colon (33%).
Considerable number of our cases had inflammatory bowel disease and malignancy. Seven patients had serious disease and simple anal disease simultaneously.
Conclusion: Significant portion of LGIB in the patients less than 40 years old are due to serious colorectal disease. So, careful approach and appropriate timing for colonoscopy is recommended
Mansour Esmailidehaj, Hassan Esmaeili, Elham Chavoushi, Mohammad Ebrahim Rezvani, Hossein Azizian,
Volume 30, Issue 4 (7-2022)
Abstract
Introduction: Numerous studies have shown that enriched plasma protects myocardial cells against ischemia. The aim of this study was to evaluate the effect of rat enriched plasma by preconditioning, ischemia, and reperfusion on the differentiation of bone marrow mesenchymal stem cells (BMMSC) into cardiomyocytes.
Methods: In this experimental and laboratory study, BMMSCs were extracted from the femur bone of male Wistar rats weighting 250-300 g. BMMSCs of the third passage were divided into six groups according to the type of plasma received: 1) control, 2) azacytidine, 3) azacytidine and non-ischemic plasma recipient, 4) azacytidine and preconditioned plasma recipient , 5) azacytidine and ischemic plasma recipient 6) azacytidine and plasma reperfusion recipient groups. In all groups except group 1, to initiate cardiomyocyte differentiation, BMMSCs were exposed to azacytidine (10 μM) for 24 hours. Plasma (25 μg / ml) was added to the culture medium each time the culture medium was changed. After 21 days, the expression level of myocardial βMHC, desmin, and troponin I genes was assessed using qRT-PCR.
Results: The expression of myocardial βMHC, desmin, and troponin I genes was significantly increased in all groups received azacytidine (groups 2 to 6) (P<0.01). There was no significant difference in the expression of myocardial βMHC, desmin, and troponin I genes, the doubling time, and migration between the plasma receiving groups (groups 3 to 6) compared to the group 2 (P>0.05).
Conclusion: The findings of present study showed that the plasma collected during ischemia, ischemia-reperfusion, and ischemia preconditioning had no effect on the differentiation of BMMSCs into cardiomyocytes in the presence of azacytidine.
Fatemeh Tavakoli, Azadeh Emami, Behrooz Heydari, Mohsen Abbasi, Mohammad Ebrahim Rezvani,
Volume 30, Issue 12 (3-2023)
Abstract
Introduction: The purpose of the present study was comparing the effects of Lipoherb with metformin on metabolic changes and sex hormones of rats with polycystic ovary syndrome
Methods: Thirty female Wistar rats were divided into 5 groups (n=6). Group (1): received normal saline for 56 days, group (2): received letrozole (1mg/kg) for 28 days and normal saline for the next 28 days, group (3): in the first 28 days, they received letrozole carrier and in the next 28 days, they received Lipoherb (320 mg/kg), group (4): they received letrozole (1mg/kg) for 28 days and Lipoherb (320 mg/kg) in the next 28 days, group (5): they received letrozole (mg/kg) for 28 days and metformin (250 mg/kg) in the next 28 days. Letrozole at the dose of 1 mg/kg for 28 days induced polycystic ovary syndrome. At the beginning and at the end of the experiment, fasting blood sugar level, lipid profile and sex hormones were measured by ELISA method. Results: Blood sugar in the letrozole plus metformin group was significantly lower than letrozole group (P<0.01). The amount of serum triglyceride and LDL cholesterol in the group that received letrozole and Lipoherb were significantly decreased compared to letrozole group (P>0.05). In the same group, total cholesterol concentration also decreased significantly compared to other groups (P>0.01). The serum level of estrogen in the group received Lipoherb was significantly lower compared to letrozole group (P<0.05). Conclusion: Compared to metformin, Lipoherb had more ability to correct the metabolic symptoms of rats with polycystic ovary syndrome. However, the hormonal effects of Lipoherb did not differ significantly from the hormonal effects of metformin in polycystic ovary syndrome.