Ashraf Moini , Farideh Malekzadeh, Behnaz Navid, Maryam Mohammadi, Reza Omani-Samani,
Volume 31, Issue 8 (11-2023)
Abstract
Introduction: Shift work disorder (SWD) is a sleep disorder that affects the circadian rhythm and is characterized by symptoms such as excessive daytime sleepiness and insomnia. This condition can significantly increase the risk of accidents and diminish the overall quality of life for individuals affected by it. Therefore, using the four-question of shift work disorder questionnaire (SWDQ) can be useful for identifying people at risk of SWD. In this research, we evaluated the accuracy and consistency of the Persian adaptation of the SWDQ among female employees.
Methods: In this descriptive cross-sectional investigation, a sampling available method was employed to select employed women who were referred to Royan Institute, Arash Hospital, and Gynecological Office in Tehran. It was used translated and reverse translation methods for the questionnaire of SWDQ. Statistical analysis was performed by using the Statistical Package for the Social Sciences (SPSS version 16, Inc. Chicago, IL, USA).The tests used in this study were Cronbach's alpha, internal correlation, and confirmatory factor analysis.
Results: In terms of content validity rate (CVR) with an average of 70% (questions 80%, 80%, 60% and 60%, respectively) and content validity index (CVI) with an average of 87.5% (questions 100%, 90%, 80% and 80%, respectively) was acceptable. The internal homogeneity of the SWD questionnaire with Cronbach's alpha was 63.4% and deleting each of the four items of the questionnaire reduced it. Moreover, the range of total correlated changes for the questionnaire was from 0.339 to 0.488, which was more than the minimum of 0.3.
Conclusion: Overall, the validity and reliability of the Persian version of SWDQ (the four-item) have acceptable.
Corresponding Author:Farideh Malekzadeh
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Farnaz Tajbakhsh, Somayeh Tavana, Mohammad Kazemi Ashtiani, Naeimeh Sadat Abtahi, Leila Sadat Tahaei, Ashraf Moini, Rouhollah Fathi,
Volume 32, Issue 10 (1-2025)
Abstract
Introduction: Ovarian tissue freezing is the most effective method to maintain fertility for immature girls and women diagnosed with cancer. Nonetheless, because of the chance that malignant cells might reappearing following tissue transplantation, it is crucial to isolate the follicles from the frozen-thawed ovarian tissue of these individuals and employ them in the process of in vitro maturation process or artificial ovarian framework. This study aimed to assess the application of neutral red (NR) vital dye alongside collagenase IA for effectively isolating viable follicles from the vitrified human ovarian tissue samples.
Methods: Two categories existed: the category with NR and the group without NR. Chopped (0.5×0.5 mm) strips of vitrified-warmed ovarian tissue from 10 transsexual individuals were placed into two falcon tubes with HTCM and Collagenase IA (1mg/ml). Neutral red (NR) was introduced to one of the falcons. Follicles were then isolated mechanically. The morphology, size, and viability of the follicles were assessed. The condition of the follicles was evaluated using fluorescent staining methods involving Calcein-AM and Ethidium homodimer-I. The t-test method was used to evaluate the data.
Results: The number of isolated follicles with Neutral Red (46.50±25/02) exceeded those without NR (6.6±5.58; P < 0/0001). Additionally, according to the morphological studies, a majority of the isolated follicles from the transsexual ovarian cortex were primordial (77.4%), and primary (21.12%) follicles, with only a small number of secondary follicles (1.4%) identified in these tissues. Live/dead staining verified the viability of isolated follicles by displaying a green hue.
Conclusion: The finding indicates that combining collagenase I with the vital dye Neutral red significantly facilitates the of follicles from dense human ovarian tissue.
