Showing 3 results for Jafary
H Baharvand , H Jafary, M Massumi , S Mollamohammadi,
Volume 14, Issue 1 (Spring 2007 2006)
Abstract
Introduction: Type I diabetes mellitus is caused by autoimmune destruction of the insulin-producing β-cells. A new potential method for curing the disease is transplantation of differentiated insulin- secreting cells from human embryonic stem cells.
Methods: Human embryonic stem cell lines (Royan H1) were used to produce embryoid bodies. Differentiation carried out by growth factor-mediated selection of nestin positive cells.
In final stage, these cells were expanded in the presence of bFGF, followed by addition of nicotinamide to promote differentiation of insulin- secreting cells.
Cells were assayed by immunocytochemistry, RT-PCR, insulin secreting assay with Radio-immuno assay kit and Transmission Electron Microscopy. The cells were transplanted into immunosuppressed mice.
Results: Analysis of differentiation cells immunocytochemistry showed that these cells were insulin, glucagon, somatostatin and pancreatic polypeptide positive. RT-PCR reaction demonstrated the expression of pancreatic endocrine genes. Differentiation cells secreted insulin in response to glucose, but no significance difference in insulin concentration was observed with an increase in concentration of glucose. The implanted cells were vascularized and remained immunoreactive with insulin and glucagon.
Transmission Electron microscopy of differentiate cells showed Golgi complexes, rough endoplasmic reticulum and a few granules but no true β granules.
Conclusion: The data showed that human embryonic stem cells can produce insulin secreting cells. However, more studies are needed to generate true beta cells.
S Jafary-Nodushan , M Shakiba , Mh Lotfi ,
Volume 16, Issue 2 (Summer 2008)
Abstract
Introduction: Water is an essential nutrient for life. It comprises 75% of total body weight in infants,60% in adult males and 50% in adult females. Decrease in body water is commonly known as dehydration. Acute or chronic dehydration is a common condition in some population groups, especialy the elderly and those who participate in physical activity in warm enviroments. Potential consequences of dehydration include constipation,urinary tract and respiratory infection,urinary stone disease and there might also be an association between a low habitual fluid intake and some carcinomas,cardiovascular disease and diabetes. Many indices have been investigated to establish their role as markers of dehydration status. Body mass changes,blood indices,urine indices and bioelectrical impedance analysis have been used most widely, but current evidence and opinion tend to favour urine indices as the most convenient and sensitive methods.
Methods: This cross sectional study was done for estimating the prevalence of dehydration in adult population(students,nurses,officials,workers)in Yazd. These persons were selected randomly. Urine samples of two hundred and thirty persons were obtained at 10-12 AM,and urine specific gravity measured by refractometer (all of the samples)and dip stick (some samples).
Finding: According to this study,96.7% of our population had some degree of dehydration.69.7% of them were significantly dehydrated(urine SG>1020) and 4.8% of them were severely dehydrated (urine SG>1030) and the mean specific gravity was 1021±5/65. This study evaluated other factors that could probably indicate hydration status like urine colour, type of drink,frequency of urination and frequency of thirst per day.
Conclusion: High percentage of our population were dehydrated which was not correlated to the type of drink but was correlated to urine colour, frequency of urination and frequency of thirst. So, regular monitoring of urine to keep if clear or light yellow is a simple way to prevent dehydration.
R Mohammad Jafary , M Barati, M Najafian, N Saadati, K Shojaie,
Volume 17, Issue 4 (atumn 2009)
Abstract
Introduction: Bleeding disorders are a common problem in daily practice, especially in premenopausal women. In our investigation, we compared the sensitivity and specificity of three different diagnostic methods Trans-vaginal sonography(TVS), saline infusion sonohystrography(SIS) and Hysteroscopy.
Methods: It was a cross- sectional study with a study population of ninety-nine women suffering fromAUB attending the outpatient clinic of Imam and Razi hospital in Ahvaz- Iran. They were evaluated by three different diagnostic methods and the results were compared. This study started from March 2008 to December 2008. The sensitivity, specificity, positive predictive and negative predictive values were measured in TVS, SIS, and finally these two methods were compared with hysteroscopy (as a gold standard).
Results: The sensitivity and specificity of TVS was 74.2% and 49.7%, respectively, while that of SIS was 91.6% and 86%, respectively. The positive predictive value and negative predictive value of TVS was 71.9% and 54.3%, respectively, while that of SIS was 85.9% and 85.75%, respectively. The sensitivity, specificity, the positive predictive and negative predictive values of SIS was more than TVS, and it was similar to hysteroscopy.
Conclusion: The findings obtained in the present study indicate that in AUB, sensitivity, specificity, positive and negative predictive value of SIS is more than TVS and therefore can be considered as a first step for diagnosis of AUB.
