Showing 3 results for Hemati
Z Khaksar, G Jelodar, H Hematian,
Volume 18, Issue 1 (March-April 2010)
Abstract
Introduction: In pregnant mothers, maternal diabetes occurs when pancreas can't produce enough insulin resulting in increased blood glucose levels in the mother and subsequently in the fetus. This investigation was conducted to evaluate the effects of maternal diabetes on cerebellum of offspring of diabetic mothers (ODM), which was carried out at the veterinary faculty of Shiraz University in 2007-2008.
Methods: This was an experimental study that included sixteen normal adult female rats divided in two groups. Diabetes was induced in one group by Alloxan agent. Both groups became pregnant by natural mating . At 7, 14, 21 and 28 days after birth, the cerebellum of all offsprings were collected and the weight of neonates was also measured. After producing histological slides, Olympus BX51 microscope and Olysia softwarwere used. Various histological parameters used included gray and white matters thicknesses (µ), the number of cells in gray and white matter separately per unit and the ratio of gray matter to white matter.
Results: Cerebellar parameters decreased in ODM as compared to the control group. The body weight of ODM was significantly more than that of the control group (p< 0.05).
Conclusions: Maternal hyperglycaemia exhibited deleterious effects on cerebellum during fetal life, which remained persistent during postneonatal period. Maternal diabetes also resulted in reduction of number of cells and thicknesses of both gray and white matter.
M Hemati, N Eivazi, A Chakerzehi, A Moradi, J Mohiti Ardakani, A Mahmoodzade,
Volume 22, Issue 2 (May-Jun 2014)
Abstract
Introduction: Understanding the molecular mechanisms of metastasis is important for the design and effective use of novel therapeutic strategies in order to combat metastases. One class of molecules that has been implicated in metastasis is Rac1. Regarding complications of conventional therapies such as chemotherapy, current studies are evaluating natural compounds with anticancer Properties. Melittin is a natural compound derived from honey bee venom which has revealed amphipatic properties. Some studies have shown that melittin has an important role in reducing cancer cells metastasis by affecting Rac1 expression. Since the studies on melittin role in regard to Rac1 in gastric cancer cells are rare, the present study examined the effect of melittin on Rac1 protein expression in AGS gastric cancer cells.
Methods: AGS cells, after growth and reaching to 80% density, were exposed to 0.2, 0.3, 0.4 μg/ml concentrations of melittin (test groups) for six hours. Then cells were collected, lysed with lysis buffer and Rac1 protein was identified by Western blotting technique. Data were normalized with the β-Actin internal control and calculated as the relative percentage. The study data were analyzed using SPSS statistical software via One-Way Anova test.
Results: Comparison of Rac1 expression levels in AGS cancer cells showed that cells which were exposed to 6 hours of 0.2μg/ml (109±5.5), 0.3(105±3.6) melittin had higher Rac1 expression percentages compared to the control (100) and 0.4 (100±2). Rac1 expression percentages were not significant with a confidence interval (CI) of 95% ( P>0.05 ).
Conclusion: In this study the results demonstrated that melittin does not have any effect on Rac1 in gastric cancer cells (AGS).
Elham Hemati, Hossein Sazegar,
Volume 32, Issue 7 (10-2024)
Abstract
Introduction: Accumulating evidence has revealed that inducing apoptosis is an important strategy to control excessive breast cancer cell proliferation. In this study, the supernatant effect of the probiotic strain of Lactobacillus Sakei on Expression Level of Apoptosis-Related Genes (Bax/Bcl2) in Breast Cancer Cell Line (MCF-7) was investigated.
Methods: In the experimental study, MCF-7 breast cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 1, 2, 3, 4 and 5 mg/ml concentrations of sakei supernatant and incubated at 24, 48 and 72 hours. The MTT assay was used to measure cytotoxicity effect according to kit manufacturer's protocol in all three incubation times. MCF-7 cells with concentration of sakei supernatant at IC50 point (5 mg / ml) to examine the expression of genes Bax and Bcl2 were incubated for 72 hours. Real-Time PCR was performed to analyze the changes in the expression of Bax and Bcl2 genes.
Results: The results of this study indicated that bioavailability of MCF-7 cell line reached the lowest value at concentration of 5 mg/ml compared to the control group. In addition, MCF-7 cell line treatment with Lactobacillus sakei supernatant at a dose of 5 mg/ml and 72 h incubation time, showed significantly increased expression of BAX (p-value= 0.0033) and significantly decreased expression of BCL-2 (p-value= 0.0278).
Conclusion: The results indicate that Lactobacillus sakei supernatant can reduce the bioavailability of breast cancer cells by inducing apoptosis pathway.
