Showing 5 results for Delfan
Shiva Ghafari, Parvaneh Nazarali, Ameneh Razavi, Maryam Delfan,
Volume 26, Issue 11 (Feb 2019)
Abstract
Background: the adipose tissue produces and releases peptides that contribute to various processes in body, including insulin resistance. The aim of this study was to investigate the effect of eight weeks of continuous aerobic training and high intensity interval training on Resistin and insulin levels and insulin resistance in type 2 diabetic male wistar rats.
Methods: Twenty-four Wistar rats became diabetic in seven months. In next phase, after introducing with training environment, Wistar rats were randomly assigned into three equal groups of six each: control, continuous (20 minutes, 60% maximum speed) and intense interval (2 minutes of activity with 80% maximum speed, 2 minutes recovery with 30% maximum speed). The rats trained five time a week for eight weeks. Resistin gene expression and plasma insulin and glucose levels were measured before and after eight weeks. One-way ANOVA was used at P<0.05 for statistical analysis of data.
Results: regardless the type of training, differences between pre and post training results was statistically significant for insulin, glucose, insulin resistance and Resistin (P<0.05). Interval training lead to significant changes in all factors except the Resistin gene expression (P<0.05). There was a significant relationship between changes in insulin resistance and Resistin gene expression (r=0.63).
Conclusion: The results of this study showed that training is an effective factor in insulin resistance process and related factors in diabetes, and Resistin also play a role in this process, but it seems that regular training is more important factor than its type to change the Expression of Resistin.
Alireza Safarnezhad, Maghsoud Peeri, Mohammad Ali Azarbayjani, Maryam Delfan,
Volume 28, Issue 7 (10-2020)
Abstract
Introduction: Regular exercise training with alternating volume fluctuatesblood glucous levels and by regulating signaling in gene expression reduces in myocardial cell apoptosis in diabetic patients. The purpose of this study was the effect of 8 weeks of high intensity interval training on the gene expression of BAX and BCL-2 in the left ventricle of diabetic rats.
Methods: The present study was a quasi-experimental one. 14 male diabetic rats were divided into 2 groups of 7; high intensity interval training (HIIT) and control (C) groups. Diabetes was induced in a pellet with a high-fat diet (30% fat and 25% fructose) for 16 weeks. Twenty-four hours after the last training and recovery session, the rats were sacrificed and their left ventricle was extracted. Glucose oxidase was used to measure glucose in plasma and insulin resistance using HOMA-IR method.. PCR-Real time was used to determine the expression of BAX and BCL-2 genes and the comparison of the groups by Independent T test was performed by Graph pad prism at alpha level of 0/05.
Results: Results showed that, BAX gene expression was significantly decreased in the HIIT group compared to the C group (P=0/0001). BCL-2 gene was significantly increased in the HIIT group compare to the control group (P=0/0001). Insulin resistance index and plasma glucose showed a significant decrease in the training group (P=0/01) (P=0/021). Weight did not change significantly in any of the groups.
Conclusion: Based on the findings, 8 weeks high intensity interval training can be reduced apoptosis in the left ventricle of Diabetic mice by decreasing the BAX gene expression and increacing the BCL-2 in myocardial and might improve diabetes cardiomyopathy.
Nadia Khayampour, Maghsoud Peeri, Mohammad Ali Azarbayjani, Maryam Delfan,
Volume 28, Issue 11 (1-2021)
Abstract
Introduction: Exercise training with different intensity regulates metabolism at the cellular level by regulating the expression of genes involved in mitochondrial biogenesis in diabetic patients. The aim of this study was to evaluate the effect of 4 weeks of high intensity interval training on the expression of PGC-1α, CS and p-53 genes in the cardiomyocytes of obese male rats with type 2 diabetes.
Methods: The present study was an experimental one. Eighteen obese male diabetic rats were divided into three groups of six: high intensity interval training (HIIT), diabetic control (DC), healthy control (NC). Diabetes was induced in all groups except the healthy control group by streptozotocin (STZ) injection. After anesthesia, blood serum was obtained directly from their left ventricle and immediately extracted from their left ventricle. Plasma glucose was measured by glucose oxidase assay. To determine the expression of PGC-1α, CS and P-53 genes, PCR-Real time method and group comparison were used by one-way ANOVA test with application 8 version graph pad prism at alpha level of 0.05.
Results: The increase in PGC-1α gene expression in HIIT group compared to DC (P = 0.0001) and NC (P = 0.001) groups was significant. Increased expression of CS gene in HIIT group was significant compared to DC (P = 0.0001) and NC (P = 0.009) groups. Decreased expression of P-53 gene in HIIT group compared to DC (P = 0.0001) and NC (P = 0.001) groups were significantly different. Weight and glucose were significantly reduced in the HIIT group.
Conclusion: The results showed that by increasing the PGC-1α, CS genes and decreasing the expression of P-53 gene in cardiomyocytes of obese diabetic rats, it improves the energy metabolism in diabetic patients due to mitochondrial deficiency and possibly it can improve diabetic cardiomyopathy.
Maryam Delfan, Fatemeh Ramzi,
Volume 29, Issue 4 (7-2021)
Abstract
Introduction: TNF-α/NF-kB signaling pathway plays an important role in promoting the inflammatory process and growth of breast cancer. Accordingly, the aim of the present study was to investigate the synergistic combination effect of endurance exercise with curcumin on breast cancer progression through TNF-α/NF-kB signaling axes in female BALB/c mice with breast cancer.
Methods: The design of the present study was experimental. After transplanting 4T1 breast cancer into the right flank of 40 BALB/c mice, all mice were randomly divided into four groups, including endurance training (E), endurance training-curcumin (EC), curcumin (CC), and control (C). Then, E and EC groups (5 days per week) were performed for 5 weeks. Curcumin (6 times per week) was given by gavage to EC and CC groups for five weeks. The intratumoral expression of TNF-α and NF-kβ were measured using the qRT-PCR method. One-way analysis of variance and LSD post hoc test with SPSS version 16 were used for statistical analysis. P< 0.05 was considered statistically significant.
Results: A significant decline in cancer growth and the gene expression of NF-kβ and TNF-α was observed in E, CC, and EC compared to the C group (P =0.001). Furthermore, a significant decline in expression of both genes was observed in EC compared with E.
Conclusion: Endurance training combined with curcumin supplementation has a more effective role in reducing TNF-α and NF-κβ, and slowing the growth of breast cancer cells when compared with either of other interventions alone.
Sara Fereshtian, Maghsoud Peeri , Hamid Agha Alinejad, Maryam Delfan,
Volume 30, Issue 8 (11-2022)
Abstract
Introduction: The aim of this study was investigating the effect of 4 weeks of high intensity interval training with curcumin supplement on the gene expression of collagen I, II and SMAD/3 in the myocardium of male obese rats with type 2 diabetes.
Methods: In this experimental study, 35 male diabetic rats were divided in to 5 groups of seven; normal control, diabetic control, curcumin+diabetic, high intensity interval training, high intensity interval training+curcumin. Diabetes was induced by an intraperitoneal injection of streptozotocin with a dose of 60 mg/kg in all groups except the normal control group. Curcumin was gavage 200 mg/kg of body weight in all groups except normal control and diabetic control groups to determine the expression of COL-I, II and SMAD/3 genes, PCR-Real time method and group comparison were used by Two-way ANOVA test at alpha level of 0.05.
Results: The decrease in COL-I gene expression in (S+HIIT (group compared to the (DC) (P<0.0001) and S+DC (P<0.0004) was significant, respectively. In high intensity interval training (HIIT) group, the decrease in COL-I gene expression was also significant compared to the diabetic control (DC) group (P=0.017).The decrease in COL-II gene expression in curcumin + high intensity interval training (S+HIIT) group compared to the (S+DC) (P=0.001) and diabetic control (DC) (P<0.0003) and in (HIIT) group was significant compared to the (DC) group (P=0.013) and (S+DC) (P=0.029).The decrease in SMAD/3 gene expression in (S+HIIT) group compared to the HIIT (P=0.008), (S+DC) (P<0.0002), (DC) groups (P<0.0006) and in the (HIIT) group was significant compared to the (NC) (P=0.010), (DC) (0.006) and (S+DC) groups (P=0.032).
Conclusion: The results showed that, high intensity interval training reduced COL-I, COL-II and SMAD/3 genes and decreasing the expression of SMAD/3 due to high intensity interval training with curcumin supplementation possibly can improve diabetic fibrosis in myocardial.
