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Showing 6 results for Azami
Evaluation and Comparison of Enzyme Immunoassay (Eia) and Acid Fast Staining with Confirmation by Immunofluorescent Antibody Assay for Detection of Cryptosporidium Species in Infants and Young Children.
D Dorostcar Moghaddam , M Azami,
Volume 12, Issue 4 (winter 2005)
Abstract
Introduction: Cryptosporidiosis is prevalent world wide, causing a variety of problems ranging from acute, self-limiting diarrhea to fatal cases in immunocompromised persons, particulary those with acquired immunodeficiency (AIDS). Diagnosis of Cryptosporidium is made by identification of oocysts in stool specimens. The detection is most commonly made by the acid-fast staining method followed by microscopic examination which has low specificity and sensitivity. Material and Methods: In the present study, we evaluated diagnostic utility of a commercially available enzyme immunoassay (EIA), which detects Cryptosporidium-Specific antigen (CSA) in 204 unprocessed stool specimens obtained from patients less than 3 years of age. Results: When compared with the routine screening procedure applied in this field study (screening by acid-fast staining and microscopy after concentration of positive results by IFA), both sensitivity and specificity were 98%. Of the 139 specimens negative by microscopy, 13 (9.3%) were positive by EIA, 11 of which were confirmed by inhibition with antibody to Cryptosporidia-specific antigen. Conclusion: The EIA is an important tool for identifying Cryptosporidium in fecal specimens in field studies since it is sensitive, specific, simple to use and unaffected by the presence of a preservative.
The Prevalence of Impaired Glucose Tolerance in Patients with Thalassemia Major in Iran: A systematic Review and Meta-analysis
M Azami, Z Tardeh, Gh Abangah, K Sayemiri,
Volume 23, Issue 10 (Jan 2016)
Abstract

Introduction: Impaired glucose tolerance (IGT) is one of the most common endocrine complications in major thalassemia patients. IGT prevalence in patients with thalassemia major has been differently in different studies in Iran, about which no total estimate can be proposed. Hence, this meta analysis study aimed to investigate IGT prevalence in major thalassemia patients in Iran.

Methods: This study was a systematic review based on electronic databases including Magiran, Iranmedex, SID, Medlib, IranDoc, Scopus, PubMed, Sciencedirect, Cochrane, Embase, Web of Science, Springer, Online Library Wiley as well as Google Scholar search engine without any time limitations up to 2015. All the potentially relevant papers were reviewed independently by two investigators using the standard key words. Then, all the reviewed studies that had inclusion criterion were evaluated. In order to statistically analyze the study data, random effect model was used via Stata software (Ver.11.1).

Results: 1346 subjects were examined in 13 studies. IGT prevalence in patients with thalassemia major was estimated 9.6 %( CI: 95%, 6.6-12.5) in Iran. The IGT prevalence in male and female major thalassemia patients were 6.5 %( CI: 95%, 1.6-11.3) and 10.2 %( CI: 95% , 6.1-14.3), respectively.

Conclusion: IGT Prevalence was demonstrated to be really high in patients with thalassemia major. As a result, implementing screening programs seems to be necessary in regard with early diagnosis of endocrine disorders especially IGT in these patients. Furthermore, it is recommended that annual glucose tolerance test be taken within such patients.


The Frequency of Vitamin D Deficiency among Referred to Clinical Laboratories in Eyvan City during 2015 and 2016- Ilam province, Iran
Sh Rahmati, Ar Yadegarazadi, Mb Bigdeli Shamloo , F Rabiei Fakhr , M Azami, M Borji, M Babashahi , Y Madmoli ,
Volume 24, Issue 3 (Jun 2016)
Abstract

Introduction: Vitamin D deficiency (VDD) causes same diseases such as osteoporosis, osteomalacia, and fractures, and also it is shown that Vitamin D deficiency could lead to some cardiovascular disease, diabetes and many types of cancer. Therefore, this study aimed to determine the frequency of vitamin D deficiency among referring to laboratories in Eyvan city during 2015 and 2016.

Methods: A cross-sectional study was conducted in 2014 and 2015 on patients who were referred to the laboratories at Eyvan city. Serum levels of 25OHD were determined using a ELISA assay. The data were analyzed SPSS 17 software using descriptive and analytical tests.

Results: Out of 2 919 participants, 2053 patients were women. The average age was 36.25±17.1 (range 1-92) yrs. The overall prevalence of vitamin D deficiency in the patients admitted to the medical laboratories was estimated that approximately 62%. The prevalence severe and moderate vitamin D deficiency was calculated 10.4 and 51.5, respectively. The prevalence of vitamin D deficiency among the ages 1-6, 7-18, 19-60 and over 60 years was estimated 45%, 59%, 64% and 58%, respectively. The mean vitamin D concentration was 36.25±18.79 ng/ml. The relationship between deficiency of vitamin D with age and gender was statistically significant (P=000).

Conclusion: Vitamin D deficiency prevalence is high, so the intervention plan seems essential to avoid complications of vitamin D deficiency such as medication treatment and establishing nationally-mandated food fortification programs to enhance the intake of vitamin D.


The Effect of an Eight Week Combined Exercise Training on Oxidative Stress and Lipid Peroxidation in Postmenopausal Women with Type 2 Diabetes
A Azamian Jazi, R Shokouhi,
Volume 24, Issue 8 (Nov 2016)
Abstract

Introduction: The basic levels of oxidative stress and lipid peroxidation is higher than normal in patients with type 2 diabetes. Therefore, due to the positive effects of exercise training (especially combined exercise training) on oxidative stress and lipid peroxidation in healthy people, the purpose of this study was to investigate the effect of an 8 week combined exercise training (Aerobic and Resistance) on oxidative stress and lipid peroxidation in postmenopausal women with type 2 diabetes.

Methods: In this semi-experimental study, 27 postmenopausal women with type 2 diabetes (age 55.30±4.1
years old, BMI 26.47±3.08 kg/m2) from Yazd city were purposefully selected and randomly divided into experimental (n=13) and control (n=14) groups. The experimental group participated in an eight week combined exercise training (three session per week), but during this period, the control group did not participate  in any exercise training program. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), fasting blood sugar (FBS) and body fat percent were measured before and after the eight-week combined exercise training. Data were analyzed using paired and independent t-test. Statistical significance was set at P < 0.05.

Results: Serum SOD and CAT levels significantly increased (p = 0.000 and p = 0.001 respectively) and the MDA levels decreased (P = 0.036) in the experimental group after 8 weeks of combined exercise training. Also, FBS (P=0.000) and body fat percent (P=0.005) decreased in the experimental group compared with the control group.

Conclusions: It seems that oxidative stress and lipid peroxidation status in postmenopausal women with type 2 diabetes in the experimental group improve after eight weeks of combined exercise training, compared with the control group.


Effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats
Mohammad Hassan Dashti Khavidaki, Akbar Azamian Jazi , Ebrahim Banitalebi, Mohammad Faramarzi ,
Volume 26, Issue 1 (Apr 2018)
Abstract
IntrodutionThe purpose of this study was investigating the effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats.
Methods: 24 male Wistar rats were randomly assigned to three groups of eight, including diabetic group with endurance exercise (D-E), diabetic (D) and healthy control groups (Con). After induction of diabetic rats by injection streptozotocin was administered intraperitoneal , endurance exercise was applied for eight weeks, three sessions pre week in diabetic rats. Exercise intensity was equal to a speed of 21-25 m / min. The relative expression of CGI-58 and ATGL protein was measured with western blot technique and serum insulin and glucose levels were measured with a specialized kit. One-way ANOVA test was performed using SPSS-20 software and at a significance level less than 5%.
Results: Results showed that ATGL and CGI-58 values were significantly different between the three groups (p <0.001). ATGL difference between the groups of diabetic group with endurance exercise with control (p = 0.001) and diabetic (p = 0.001)was significant. CGI-58 difference between the groups of diabetic group with endurance exercise with control (p = 0.001) and diabetic (p = 0.002) was significant. In addition, serum glucose and insulin levels decreased significantly after eight weeks of training (p <0.05).
Conclusion: It seems that CGI-58 play a vital role in activating lipolysis by ATGL and increasing in CGI-58 leads to an increase in ATGL and ultimately leads to increased levels of intramuscular triglyceride oxidation and improved insulin resistance.
Antitumor Activity and Immunomodulatory Effects of Ficus carica Latex
Hamideh Azami, Saeed Malek-Hosseini, Mayam Mojahed Taghi, Mohammadrasul Zareinejad, Zahra Amirghofran,
Volume 28, Issue 12 (2-2021)
Abstract
Introduction: There are limited studies on the anti-cancer and immunomodulatory effects of the fig fruit latex. In this study, we aimed to investigate the effect of fig fruit latex on several cancer cell lines as well as its effect on lymphocytes proliferation and cytokines secretion.
Methods: After preparing a methanolic extract from fig latex, its effect on various cancer cell lines including Fen (bladder cancer), K562 (myeloid leukemia), Hela (cervix carcinoma), Jurkat (lymphoid leukemia) and Raji (lymphoma) was examined by MTT colorimetric assay. For evaluating the effects of extract on lymphocyte proliferation and viability, BrdU assay and flow cytometry staining were used. Cytokine secretion was measured by ELISA assay.
Results: The extract showed the strongest activity against K562 cell line (IC50, 234 µg/ml) and the least activity against Hela cells (IC50 >1000 µg/ml). On evaluation of the immunomodulatory effect of fig by BrdU assay, a reduction in lymphocytes proliferation by increasing the concentration of the extract was observed; proliferation index from 1.2±0.06 at 0.1 µg/ml of the extract reached to 0.13±0.2 at 800 µg/ml. In flow cytometry analysis, a significant cytotoxic effect at concentrations ≥400 µg/ml was observed. The extract at 100 and 200µg/ml had the ability to reduce secretion of interferon (IFN)-γ and interleukin (IL)-4 cytokines.
Conclusion: Fig latex extract showed cytotoxicity on different cells particularly K562 leukemia cells which implied its anticancer activity. This extract at lower concentrations reduced lymphocytes proliferation and cytokine production which showed its immunoinhibitory effects and suggested its possible beneficial in treatment of immune-mediated diseases.

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