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Showing 2 results for سلطان دلال

Mm Soltan Dallal, S Vahedi, H Zeraati, R Bakhtiari, F Izadpoor, M Khalifeh Gholi, Z Rohani Rankohi, H Norooz Babaei, Tm Kaffashi, P Fazeli, A Kamkar,
Volume 15, Issue 1 (Spring 2007)
Abstract

Introduction:Despite advances in disease prevention and food materials technology, food – borne diseases are still a major problem in both developed and developing countries. Morever, meat plays a key role in transfer of bacteria, especially “Zoonotic” to humans. Therefore, we decided to investigate the outbreak of pathogenic bacteria such as Salmonella,Campylobacter, Yersinia and Aeromonas in red meat and chicken offered as packed and unpacked in areas under the authority of Tehran university of medical sciences . Methods: 630 samples including 315 raw chicken meat and 315 raw red meat samples were collected and tested for a period of one year from July, 2004 to August,2005. Samples were collected from shops selling packed meat and chicken as well as shops selling unpacked meat and chicken in different parts of the south of Tehran The methods used for the laboratory investigation were based on Iranian National Standard Procedure No. 2394. Results: Of the 630 samples of chicken and meat, 183 samples (29 %) were contaminated. 49.2 percent of the contaminated samples were chicken meat and 8.9 percent were red meat. From the total, 71 samples were contaminated with salmonella (11.3 %), 68 samples with Campylobacter (10.8 %), 26 samples with Yersinia entrocolitica (4.1 %) and 18 samples with Aeromonas (2.9 %). In red meat samples, microbial contamination was observed in 4.9 % of packed and 10.3 percent of unpacked samples. Contamination rate of chicken samples was higher including 59.3 % of packed and 45.7 % of unpacked chicken samples. The observed difference between the remitting samples of packed and unpacked chicken was statistically significant. (P< 0.05) Conclusion: Our results indicated that although the centers selling packed and unpacked red meat from south of Teheran showed different microbial contamination rate, the differences were statistically insignificant. (P> 0.05)
A Akbari , Mr Pourmand , F Fard Sanei , N Mardani , Mm Soltan Dallal ,
Volume 17, Issue 4 (atumn 2009)
Abstract

Introduction: Shiga toxin- producing Escherichia coli (STEC) is an emerging bacterial pathogen in developing countries that causes several diseases such as diarrhea, hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS), particularly in children. Aim of the research was detection of STEC in diarrheal specimens from under 5 year olds and study of the patterns of antibiotic resistance of these strains. Methods: In the study,300 fecal samples were collected from children with diarrhea referring to Ali Asghar Hospital. E.coli species were isolated by standard bacteriological and biochemical tests. Presence of shiga toxin genes (stx1/2) was investigated by PCR technique (Qiagen). Antibiogram test for strains containing the toxin gene was performed using 16 different antibiotic discs (MAST) by disc diffusion agar (Kirby-Bauer) method. Results: From 39 E.coli isolates, 9(23.1%) strains were detected by PCR to contain stx1/2 gene. One strain was resistant to all 16 antibiotics. All the STEC strains were sensitive to meropenem (MRP), imipenem (IMI), gentamycin (GEN) and nitrofurantoin (NI). 4(44.44%) strains showed multi-drug resistant pattern. All these 4strains were resistant to cotrimoxazole(SxT). Also, 6(66.66%) strains were resistant to at least one antibiotic. Conclusion: In Iran, shiga toxin- producing Escherichia coli (STEC) may be a commonly bacterial pathogen causing diarrhea, particularly in children. Therefore, we should use new techniques for investigation of these strains. Increase in number of emerging and new strains that could be resistant to classic antibiotics such as cotrimoxazole may be foreseen. It is suggested that antibiotics prescription programs in treatment of diarrhea causing E.coli strains be updated.

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