J Hossein Zadeh, Mb Khalili, Ms Golvardi, M Sadeh, Mh Sadr-Bafghi, Ps Mohseni- Meybodi,
Volume 16, Issue 1 (Spring 2008)
Abstract
Introduction: Many post-surgical wound infections in hospitals cause morbidity and morality of patients and these are usually transmitted via hands of surgical personnel. The aim of the present study was to detect and antibiogram the bacterial agents following scrubbing of hands of surgical personnel before operation.
Methods: Hands of 134 personnels of operation room were swabbed following scrubbing with antiseptic Betadine solution. Swab samples were inoculated on selective and differential media such as blood ager, McConky and manitol salt agar(MSA). Following incubation of media at 37c° for 24hr, bacterial species were identified using differential related tests. The isolated species were than antibiogramed and the results together with other data was analysed by SPSS software program.
Results: Of the total of 134 cases, 81(60.4%) were male and 53(39.6%) female. The mean scrub time for each person was (206.1+/-103.2) seconds 6 to 60 seconds base change. Increasing time of scrub was significantly correlated with decreasing rate of bacteria (P=0.003), (R=-0.254). Contamination was present in 129(96.3%) cases following scrubbing. Maximum contamination was observed in nails (92.5%). Average number of bacteria for each individual was between 0 and 159. 62.6% of isolated bacteria were non- staphylococci and 7.7% were S. aureus. Vancomycin and ceftizoxim were the most sensitive, while penicillin was the least sensitive antibiotic.
Conclusion: Results revealed that hand contamination was more than the expected standard level. Therefore, regarding the critical task of surgical personnel, training of all operation room staff is highly recommended to minimize the rate of contamination.
Mehdi Fatahi Bafghi, Hengameh Zandi, Maryam Sadeh,
Volume 32, Issue 8 (11-2024)
Abstract
Introduction: The genus Rhodococcus, first described by Zopf in 1891, comprises 56 species with validly published names. This bacterium belongs to the phylum Actinobacteria, class Actinobacteria, order Mycobacteriales, and family Nocardiaceae. Notable characteristics of this bacterial genus include: Gram-positive, partially acid-fast positive, catalase-positive, non-spore-forming, and non-motile. The use of molecular methods such as 16S rRNA gene sequencing, along with phenotypic methods, has been suggested for the identification of this bacterium. To date, nine species of the genus Rhodococcus have been reported from clinical specimens. Currently, microbroth dilution is the standard method for assessing the antibiotic susceptibility testing of Rhodococcus species. The pattern of antibiotic resistance in Rhodococcus species varies based on geographical region, and it is recommended that antibiotic susceptibility testing be performed for clinical isolates.
