R Davoodi, S Esmailzadeh Bahabadi, Sh Najafi, M Mazaheri,
Volume 23, Issue 5 (8-2015)
Abstract
Introduction: Breast cancer is regarded as one of the most common female cancers in the world. Many efforts have been made to treat the breast cancer, among which surgery, radiation, and chemotherapy can be mentioned. There is an increasing interest regarding using herbal preparations as a promising source of new anti-cancer drugs because of their safety profile and efficient pharmaceutical potentials. Since citrullus colocynthis proposes some cytotoxic effects against cancer, the present study aimed to explore the effect of hydro alcoholic extract of C. colocynthis fruit on the viability and expression level of Caspase-3 in MCF-7 cell line.
Methods: In this laboratory study, the MCF-7 breast cancer cells were treated by different concentrations of Citrullus Colocynthis ethanol extract for 24, 48 and 72 h. The extract effect on cell viability was assessed by trypan blue staining. The RNA extraction was performed, and after the construction of cDNA, expression of Caspase 3 was analyzed via Real-Time PCR.
Results: The obtained results revealed a noticeable dose and time dependent reduction in viability of C. colocynthis in the treatment group compared to the control samples. Moreover Real-Time PCR results demonstrated that the expression of Caspase3 gene at 48 and 72 hours after extract treatment significantly increased compared to the control cells.
Conclusion: It can be concluded that citrullus colocynthis fruit extract can destroy cancer MCF-7 cells, which is resulted from an increase in caspase 3 gene expression.
Kh Onsory, N Vahabi Barzi, Z Haji Mehdi Nouri, M Mousavi,
Volume 23, Issue 7 (10-2015)
Abstract
Background: A series of biochemical reactions are involved in the endogenous production of estrogens. Their final and rate-limiting step is catalyzed by aromatase belonging to the class XIX of cytochrome P450. CYP19 is a key enzyme for estrogen synthesis in males. It catalyzes the irreversible conversion of androstenedione and testosterone to estrone and estradiol-17&beta, respectively. Aromatase P450 is present in the endoplasmic reticulum of estrogen-reproducing cells in which it is expressed. The effects of the resulting estrogens are mediated through the estrogen receptor. One of the most important polymorphism, is a C to T variation in exon 7 resulting in an Arg264Cys amino acid exchange, has been shown to be very common in Asia. The purpose of this study was to determine the association of CYP19 gene polymorphism with the prostate cancer risk among the studied population. Methods: PCR-RFLP analysis of CYP19 gene was on 100 prostate cancer patients and an equal number of matching controls. The data was analyzed using the computer software SPSS for windows (version 19). Results: The frequency of CT genotype was higher in patients (37%) as compared to controls (21.2%) and this incidence was statistically significant (OR, 2.10 95 % CI, 1.02-4.34 P=0.044). Stratification of patients according to the risk factors, resulted in a slightly improved OR in individuals carrying CT compared to CC genotype (OR, 2.35 95% CI, 1.11-4.96 P=0.024). The TT genotype was not significantly associated with prostate cancer risk (OR, 0.63 95% CI, 0.16-2.50 P=0.519). Conclusion: It seems that CT genotype is more associated with cancer prostate compare with other genotypes. It appears to be an increased risk of prostate cancer associated with the Arg264Cys substitution in the CYP19 gene.
M Kolahi , Mr Tabandeh , S Saremy , Sa Hosseini , M Hashemitabar ,
Volume 24, Issue 3 (6-2016)
Abstract
Introduction: Polyphenolic compounds have anti proliferative and induced apoptotic features on cancer cells. p-Coumaric acid can be abundantly found in fruits, vegetables, plant production and honey. . Breast cancer is the most frequently diagnosed cancer among women in the world. This study aimed to investigate the effect and mechanism of p- coumaric acid on apoptosis of MCF-7 breast cancer cells.
Methods: In order to study appoptic effect of p- coumaric acid, MCF-7 breast cancer cells were treated with different concentrations of p- coumaric acid (10, 37, 70, 150 and 300 mM) for 24 h. Cell viability was determined using MTT assay. Apoptosis markers including phosphatidylserine exposure at the outer leaflet of the plasma membrane were measured using flow cytometery for Annexin V affinity.
Results: Cell viability of MCF-7 cells was decreased with increasing of p- coumaric acid concentration. Maximal effect of p- coumaric acid was observed in cells that treated with 300 mM for 24h (p< 0.05). Viability assay showed that the IC50 of p- coumaric acid in MCF-7 cells was about 40 mM. p- coumaric acid at dose of 300 mM significantly increased the late apoptotic cells with Annexin V+ and propium iodide (PI+) features after 24 h treatment.
Conclusion: The results of this study showed that p- coumaric acid had effective appoptic activity against MCF-7 cells. The results can be helpful in understanding the anticancer mechanism of p- coumaric acid and using it was suggested as an alternative or complementary drug in cancer chemotherapy.
Ns Hosseini , Mai Dehqani, S Mahmel Za Yazdi, F Amiri,
Volume 24, Issue 7 (10-2016)
Abstract
Introduction: Nowadays, over-ordering of blood is a common practice in preparation for elective surgical procedures that due to the blood products shortage and and the fact that high amount of blood is not used, this problem can be solved by changing the blood ordering schedule. The aim of this study was the examination of stored blood transfusion rate in elective surgical procedures.
Materials and Methods: This present cross-sectional study was undertaken on 296 patients who were chosen by the use of non-random method underwent elective surgeries C/ S, thyroidectomy, total hysterectomy, laminectomy, open cholecystectomy, Spilenectomy, total hip and total Knee between September 2013to march 2014. Information was extracted by check list researcher and patient’s records and it was analyzed by the means of descriptive statistics and statistical software 21 SPSS.
Results: A total of 296 patients underwent chosen elective surgery during the study period. A total of 912 blood units were cross-matched of which only 115 units were transfused. This means only 12.6% of units were transfused while 87.4% of blood units were not used. Also, no significant correlation was found between the patient's age, gender and his/her blood utilization.
Conclusion: According to the findings, routine bloods reservation in chosen elective surgeries are not necessary. In order to improve the quality of ordering, the use of "maximum surgical blood ordering schedule" is suggested, by applying this, it could fit the varying demands of bloods of hospital
J Firouzi, M Ebrahimi, F Sotoodehnejadnematalahi,
Volume 25, Issue 2 (5-2017)
Abstract
Introduction: The cancer stem cells are the small population of cells in tumor tissue with the ability of self-renew and differentiation into other tumor cells. Targeting these cells has great importance in the treatment of cancer and prevent cancer recurrence. Milk thistle is the plant of the Asteraceae with the scientific name of Silybum Marianum. However there is no report about the effect of Silibilin on mammospheres. So, the aim of this study was to evaluate the effect of silibinin on the ability of self-renewal, growth and stemness genes expression and markers of MCF-7 cell line derived mammospheres.
Methods: In this study, the viability of the MCF7 derived mammospheres were treated with silibinin for 72h using MTS test and 50% lethal dose (IC50) have been evaluated, respectively. Next, untreated and treated mammospheres with silibinin were investigated for the ability of invasion, colony and sphere formation, expression of stemness genes expression includes Oct4 and KLF by real time PCR.
Results: Our data showed that silibinin decreased the MCF-7 cells in the mamospheres with a dose dependent manner. Silibinin at dose of 150 µM (IC50) reduced 4.6 and 11.2 fold the ability of sphere and colony formation respectively. Also invasion and expression of stemness genes significantly decreased.
Conclusion: Due to reduction of growth, colony and sphere formation, as well as reduction of invasion and expression of stemness genes, Silibinin can be a good candidate for targeting of cancer stem cells.
Frida Seyedmir , Kamal Mirzaie, Morteza Bitaraf Sani,
Volume 25, Issue 4 (7-2017)
Abstract
Abstract
Introduction:
Decision tree is the data mining tools to collect, accurate prediction and sift information from massive amounts of data that are used widely in the field of computational biology and bioinformatics. In bioinformatics can be predict on diseases, including breast cancer. The use of genomic data including single nucleotide polymorphisms is a very important factor in predicting the risk of diseases. The number of seven important SNP among hundreds of thousands genetic markers were identified as factors associated with breast cancer. The objective of this study is to evaluate the training data on decision tree predictor error of the risk of breast cancer by using single nucleotide polymorphism genotype.
Methods:
The risk of breast cancer were calculated associated with the use of SNP formula:xj = fo * In human, The decision tree can be used To predict the probability of disease using single nucleotide polymorphisms .Seven SNP with different odds ratio associated with breast cancer considered and coding and design of decision tree model, C4.5, by Csharp2013 programming language were done. In the decision tree created with the coding, the four important associated SNP was considered. The decision tree error in two case of coding and using WEKA were assessment and percentage of decision tree accuracy in prediction of breast cancer were calculated. The number of trained samples was obtained with systematic sampling. With coding, two scenarios as well as software WEKA, three scenarios with different sets of data and the number of different learning and testing, were evaluated.
Results:
In both scenarios of coding, by increasing the training percentage from 66/66 to 86/42, the error reduced from 55/56 to 9/09. Also by running of WEKA on three scenarios with different sets of data, the number of different education, and different tests by increasing records number from 81 to 2187, the error rate decreased from 48/15 to 13/46. Also in the majority of scenarios, prevalence of the disease, had no effect on errors in the WEKA and code.
Conclusion:
The results suggest that with increased training, and thus the accuracy of prediction error decision tree to reduce the risk of breast cancer increases with the use of decision trees. In Biological data, decision trees error is high even with a 66/66% training. On the other hand by increasing the number of SNP from 4 to 7 decision tree, decision tree error dramatically decreased at 70/1% training. In general we can say that with increased training and increasing the number of SNP in the decision tree, the prediction accuracy increased and errors reduced. In the CODING and WEKA, percentage of disease prevalence had no significant effect on errors,” Because of selecting set of training and testing by systemic method “.
Mostafa Eghbalian, Sasan Amiri, Ghodratollah Roshanaei, Hossain Esfahani, Mohsen Ahmadi, Negar Assadi Sajadi,
Volume 25, Issue 11 (2-2018)
Abstract
Introduction: Childhood cansers are the second leading cause of death among children. Since the incidence and prevalence of malignancies are different in different regions and there are several cultural and environmental factors involved, the aim of this study was investigating epidemiology of common malignancies in children in Hamedan Province during the years of 1386-1395.
Methods: In this cross-sectional study, the population study was the children with malignancies referred to Besat's Hospital in Hamadan Province. The collected data were analyzed using SPSS 20 software. Descriptive indices and Chi-square test were used in this study.
Results: All available records showed that the malignancy was more prevalent in boys in freqency (57/7%). Most acute lymphoblastic leukemia (ALL) patients were 1-9 years, most acute myeloid leukemia (AML) patients were older than 9 years and most other malignancies were 1-4 years and over 9 years. The number of blood platelets (PLT) and the white blood cells (WBC) of the majority of patients in other malignancies, respectively, were greater than 100,000 and less than 50,000. For acute lymphoblastic leukemia and acute myeloid leukemia patients, the number of PLT and WBC were less than 100,000 and less than 50,000, respectively. The most clinical symptoms for acute lymphoblastic leukemia patients were fever and pale, fever and bleeding for acute myeloid leukemia AML patients, and finally masses and fever for patients with other malignancies.
Conclusion: Although the results of the family cancer were consistent with expected results concluded of similar studies, but the incidence of other malignancies in females was different (51/1%) with the results of some other studies.
Masoud Zare Shehneh, Seyed Mehdi Kalantar, Mohammad Hasan Sheikhha, Amir Asri Kojabad, Bibi Fatemeh Haghiralsadat,
Volume 27, Issue 1 (5-2019)
Abstract
Introdution: Nanotechnology introduced new methods to chemotherapy drugs delivery into cancer cells to reduce the side effect of drugs it increases the quality of cancer treatment and reduces the side effects of chemotherapy. . In this study, different lipid formulations of nucliposomes containing curcumin was prepared by thin-layer method and evaluated for chemical-physical evaluation on MCF-7 cell line.
Methods: This study was a descriptive-analytic. Various formulations of liposomes prepared from Span 60, SPC and cholesterol. Curcumin was loaded in the liposomes. Final liposome formulation selected and PEGylated. The Optimal formulation characterized by DLS, FT-IRT, Fe-SEM and SEM. Statistical analysis was performed with Prism6 software.
Results: The average size of liposomes, encapsulation efficacy of liposomal loaded curcumin and Zeta potential was about 63.9 nm, -41mV and %95.58, respectively. Liposomes exhibited the slowest release rate in normal and turmeric cell culture condition. To compare the function of free curcumin in similar concentrations, liposomal curcumin formulation was %63 toxic to MCF7 Cell line.
Conclusion: The present study shows that an optimally optimized nanoliposome with curcumin having appropriate physicochemical properties, improving drug toxicity in cancerous cell lines and increasing drug stability can be a good vector for drug delivery to breast cancer cells.
Conclusion: The present study shows that an optimally optimized nanoliposome with curcumin having appropriate physicochemical properties, improving drug toxicity in cancerous cell lines and increasing drug stability can be a good vector for drug delivery to breast cancer cells.
Shiva Mosadegh Manshadi, Fatemeh Nadali, Mohammad Reza Shams Ardekani,
Volume 29, Issue 3 (5-2021)
Abstract
Introduction: Prunus armeniaca is one member of the Rosaceae family. Antitumor activity of this family on the colon, prostate, bladder, cervix, lung and breast cancers has been proven in previous research, which is attributed to a natural compound called amygdalin. Therefore, in this study, we investigated the effect of apricot kernel extract on acute leukemia that has not been studied before.
Methods: In this experimental study, Aqueous, ethyl acetate, and hydro alcoholic of the Armeniacae semen extracts were prepared. The NALM-6 and KG-1 cell lines were treated with different doses of extracts; cell viability was investigated with MTT test and using double staining fluorescent, cell apoptosis was detected. Cell cycle progression was analyzed, using a BD Cycle TEST PLUS DNA Kit.
Results: The results showed that different extracts of Prunus armeniaca induced dose-dependent inhibition in both Kg1 and Nalm6 cell lines. According to the results of MTT test, the strongest IC50 in Kg1 and Nalm6 cell lines was related to the 48h effect of ethyl acetate extract of Prunus armeniaca. Fluorescent microscopy analysis showed apoptosis. The ethyl acetate extract application in both cell lines increased the number of cells in the G0 / G1 phase and decreased the number of cells in the G2 / M phase.
Conclusion: It seems that ethylacetat extract of Prunus armeniaca can reduce the proliferation of Kg1 and Nalm6 cell lines, probably by regulates cell cycle progression.
Maryam Delfan, Fatemeh Ramzi,
Volume 29, Issue 4 (7-2021)
Abstract
Introduction: TNF-α/NF-kB signaling pathway plays an important role in promoting the inflammatory process and growth of breast cancer. Accordingly, the aim of the present study was to investigate the synergistic combination effect of endurance exercise with curcumin on breast cancer progression through TNF-α/NF-kB signaling axes in female BALB/c mice with breast cancer.
Methods: The design of the present study was experimental. After transplanting 4T1 breast cancer into the right flank of 40 BALB/c mice, all mice were randomly divided into four groups, including endurance training (E), endurance training-curcumin (EC), curcumin (CC), and control (C). Then, E and EC groups (5 days per week) were performed for 5 weeks. Curcumin (6 times per week) was given by gavage to EC and CC groups for five weeks. The intratumoral expression of TNF-α and NF-kβ were measured using the qRT-PCR method. One-way analysis of variance and LSD post hoc test with SPSS version 16 were used for statistical analysis. P< 0.05 was considered statistically significant.
Results: A significant decline in cancer growth and the gene expression of NF-kβ and TNF-α was observed in E, CC, and EC compared to the C group (P =0.001). Furthermore, a significant decline in expression of both genes was observed in EC compared with E.
Conclusion: Endurance training combined with curcumin supplementation has a more effective role in reducing TNF-α and NF-κβ, and slowing the growth of breast cancer cells when compared with either of other interventions alone.
Atefeh Soltani, Narges Abbasi, Aliakbar Pourfathollah, Maryam Eskandari Mehrabadi,
Volume 29, Issue 11 (2-2022)
Abstract
Introduction: Hypertension is one of the most important preventable risk factors for cardiovascular disease. This observational study aimed to depict the role of regular blood donation on the systolic and diastolic blood pressure (SBP and DBP, respectively) in hypertensive patients in comparison with normotensive people.
Methods: This study was performed as a cross-sectional descriptive study on 546 blood donors of Vesal Blood Donation Center in Tehran who donated between 1 and 4 times in the period of 2016-2017. Donors were divided into two groups (hypertension and normal blood pressure) based on the systolic blood pressure (SBP) and diastolic blood pressure (DBP) at the time of the first donation. The trend of changes in blood pressure after each donation (second, third and fourth donation) was assessed using SPSS ver. 16 and One way-Anova statistical test.
Results: In the group of hypertensive patients,, after four blood donations, systolic and diastolic blood pressure showed a statistically significant decrease after three or four donations.
Systolic blood pressure in this group after the fourth donation decreased from the mean of 149.1 to 141.6 mm Hg (P = 0.03) and diastolic blood pressure decreased from 90.04 to 86.08 mm Hg (P = 0.04 also), respectively. Decreased blood pressure was also observed in the normal group, although this significant decrease was not reported (P> 0.05).
Conclusion: Our results corroborated a down-regulatory effect of regular blood donation on BP in hypertensive people. These findings are rather encouraging people toward voluntary blood donation and can be considered in therapy-related issues.
Hossein Karami, Mehrnoush Kosaryan, Mohammad Naderisorki, Fatemeh Esfandiari, Mobin Ghazaiean, Aily Aliasgharian, Hanie Fallah, Salameh Shakeri, Hadi Darvishi-Khezri,
Volume 31, Issue 6 (9-2023)
Abstract
Introduction: OsteoPenia and osteoPorosis are known to be one of the main comPlications of β-thalassemia major (β-TM). The aim of this study was to determine the factors associated with decreased bone density of these Patients.
Methods: Lumbar and femoral neck bone mineral density (BMD) in β-TM Patients characterized via Dual-energy X-ray absorPtiometry (DXA) to PinPoint the association of iron chelators, diabetes mellitus, serum ferritin above 3000 ng/mL, hemoglobin below 8 gr/dl, using hydroxyurea, liver siderosis and moderate to severe hePatic and cardiac siderosis with BMD. The information was collected from the thalassemia registry in 2019 and statistical analysis was Performed by STATA-13 software.
Results: Out of 1959 ParticiPants with β-thalassemia major registered in the registry, 139 ones (7.09%) had bone mineral density (53 males and 86 females). The Prevalence of abnormal bone density in the lumbar and femoral neck was (52.48 to 47.66) 57.5 and (40.18 to 57.52) 48.85 Percent, resPectively. After adjusting for the effects of Possible interventions, the odds ratio of abnormal bone density in the Patients consuming hydroxyurea and deferasirox were 0.24 (0.03 to 1.73) and 0.25 (0.03 to 1.69), resPectively. The highest odds were 8.21 (0.18 to 360.22), and 6.72 (0.60 to 74.83) for moderate to severe cardiac and hePatic dePosition, resPectively.
Conclusion: The Prevalence of osteoPenia and osteoPorosis is high among thalassemia major Patients. ConsumPtion of hydroxyurea and deferasirox is the most imPortant Protective factor and moderate to severe cardiac and hePatic siderosis are the most imPortant risk factors for abnormal bone density.
Bahare Fallah, Fatemeh Fathi, Ahmadreza Fallahfaragheh, Khadijeh Nasiriani, Akram Mehrabbeik,
Volume 31, Issue 12 (3-2024)
Abstract
Introduction: Breast cancer has been recognized as the most common malignancy in women during the last 10-15 years. Quality of life is the result of several physical and psychosocial effects. The result of this study was conducted to investigate the strategies of mental adjustment to cancer and its impact on the quality of life of breast cancer patients.
Methods: This descriptive-correlational study was conducted on 240 breast cancer patients using simple random sampling who were referred in Shahid Ramezanzadeh Radiotherapy Center, Yazd, Iran, from April to October 2021. Data collection tools were standard quality of life questionnaires (EORTC QLQ-C30) and Mini-Comparison of Mental Adaptation to Cancer (Mini-MAC). Data were analyzed using t-test, correlation and regression in SPSS version 16 software.
Results: The mean age of patients was 44.77(±11.00) years. There was a significant and negative correlation between quality of life and Helplessness hopelessness (r= -.795, p<0.001) and anxious preoccupation (r= -.705, p<0.001). Quality of life was significantly and positively correlated with fighting spirit (r= .368, p<0.001) and cognitive avoidance (r= .364, p<0.001) and Fatefulness(r= .155, p<0.05). In general, cancer coping strategies explained 59% of the quality of life variation in breast cancer patients.
Conclusion: In order to improve the quality of life of patients, it is necessary to teach them adaptive strategies to adjust with cancer, such as fighting spirit and cognitive avoidance.
Mehran Beyki Dehabadi, Vajihe Akbari,
Volume 32, Issue 3 (6-2024)
Abstract
Introduction: Using the immune system responses opens new horizons for cancer treatment. Immune checkpoints are receptors on the surface of cells that prevent the immune response against self-antigens. Cancer cells overexpress these receptors to avoid the immune system's response against them. CTLA-4 and PDL-1/PD1 are examples of immune checkpoints that are expressed on breast cancer cells. PDL-1 expression on breast cancer cells is associated with larger size, progression, more differentiated tumor, which is inversely related to the patient's survival rate. But by inhibiting this pathway with drugs such as Atezolizumab or Ipilimumab, it is possible to inhibit the escape mechanism of tumor cells from the immune system.
Conclusion: Medicines such as checkpoint inhibitors that activate the immune system to face tumors are a new way to help treat breast cancer. In cases where the usual treatment instructions do not have a suitable response, it is possible to improve the survival of patients by adding this family of drugs in the treatment process and considering the side effects as well as their effectiveness.
Alireza Khanahmad, Mohammad Hossein Khazaee-Nasirabadi,
Volume 32, Issue 4 (7-2024)
Abstract
Introduction: Exosomes, small microvesicles of endosomal origin, play a pivotal role in intercellular communication and various physiological and pathological processes. All cell types, especially cancer cells, release exosomes. These vesicles can induce tumor progression, metastasis, and drug resistance by altering the tumor microenvironment and transferring their content to target cells. Exosomes contain lipids, proteins, and genetic biomolecules such as DNA, mRNA, and miRNA with their contents varying depending on cell type and disease state. Despite their role in cancer progression, exosomes also show potential as therapeutic agents. They are effective carriers for targeted drug delivery due to their high biocompatibility and low immunogenicity. However, more extensive clinical trials are needed to evaluate the efficacy and safety of exosome-based therapies. Oncology researchers are encouraged to investigate exosome contents more precisely and design new therapeutic strategies utilizing these vesicles.
