Evaluation of malone-di-aldehide as an indicatorfor semen bacterial contamination

malek shahi, masoumeh; razavian, seyed mohammad hossein; Aghaee, seyed soheil

Volume 26, Issue 5 (Agu 2018) JSSU 2018, 26(5): 426-438 | Back to browse issues page

Mendeley

Zotero

RefWorks

malek shahi M, razavian S M H, Aghaee S S. Evaluation of malone-di-aldehide as an indicatorfor semen bacterial contamination . JSSU 2018; 26 (5) :426-438
URL: http://jssu.ssu.ac.ir/article-1-4442-en.html

Evaluation of malone-di-aldehide as an indicatorfor semen bacterial contamination

Masoumeh Malek shahi

, Seyed mohammad hossein Razavian ^*

, Seyed soheil Aghaee

Abstract: (3201 Views)

Introdution: The statistics of infertility among couples is about 15%. 60% of infertility cases are related to male factors, and the share of infertility due to genital tract infection is 20%. In bacteriospermia, the presence of bacteria and leukocytes produce active forms of oxygen and damage to sperm cells. In the present study, determination of frequency and diversity of bacteria involved in urinary tract infection, determination of malondialdehyde in sperm and seminal plasma as an index of oxidative damage and its relation with microbial contamination and effect of oxidative stress and microbial contamination on the quality of semen was evaluated in infertile men.
Methods: This trans-sectional study performed on 53 patients referred to the Infertility Treatment Center of Jahad Daneshgahi. After sampling, semen parameters according to WHO (1999) protocol and bacterial contamination by culture and differential tests and also malondialdehyde concentration in sperms and seminal plasma via fluorimetric method were determined and results analyzed by ANOVA and T test in SPSS16 software.
Results: Based on the results, 92% of the patients had bacterial contamination, including Staphylococcus aureus (65%), Staphylococcus epidermidis (24.5%), Proteus mirabilis (2.4%), Klebsiella (4.8%) and Enterococcus (4.8%). Bacteriospermia had not only a significant effect on the quality of semen, especially sperm motility (p<0.01), but significantly increased (p<0.01) the malondialdehyde concentration in patients' cells and seminal plasma.
Conclusion: Infection affects semen quality and decreases the chance of a successful ART. Malondialdehyde can be used as a quick and accurate indicator for evaluating bacterial contamination in infertile men.

Keywords: Infertility, Bacteriospermia, Reactive oxygen spices, Malondialdehyde.

Full-Text [PDF 1081 kb] (843 Downloads)

Type of Study: Original article | Subject: Biochemistry
Received: 2018/01/8 | Accepted: 2018/02/10 | Published: 2019/04/8

References

1. 1- Guyton AC. Human Physiology and Mechanisms of Diseases. 5th edition. W.B. Saunders Publishers 1992; 690-99.

2. 2- World Health Organization (WHO). Laboratory manual for the examination of human semen and semen-cervical mucus interaction. 4th edition. Cambridge Uni Press 1999; 4-23.

3. 3- Ganong W F, Review of Medical Physiology, 22nd Edition. McGraw Hill Company 2005; 912-943.

4. 4- Gagnon C, Lamirande E. Extrinsic factors affecting sperm motility: Immunological and infectious factors and reactive oxygen species in male sterility and motility disorders. Springer. New York 1999; 37-44.

5. 5- Hull M G, Glazener C M, Conway D I, Foster P A, Hinton R A, Coulson C. Population study of causes treatment and outcome of infertility. Br Med J 1995; 291(6510):1693-97.

6. 6- Christiansen P. Infection in the male reproductive tract, Impact, diagnosis and treatment in relation to male reproductive infertility. Int J Androl 1993; 16: 1-13.

7. 7- Gomez CI, Stenback WA, James AN, Criswell BS, Williams RP. Attachment of Neisseria gonorrhoea to human sperm, Microscopical study of the effect of trypsin and iron. Br J Vener Dis 1979; 55(4): 245-55.

8. 8- Keck C, Gerber S C, Clod A, Wilhelm C, Breckwoldt M. Seminal tract infection; impact on male fertility and treatment options. Hum Repord update 1998; 4(6): 891-903.

9. 9- Virecoulon F, Wallet F, Fruchart-Flamenbaum A, Rigot J M, Peers M C, Mitchell V, et al. Bacterial flora of the low male genital tract in patients consulting for infertility. Andrologia 2005; 37(5): 160-65.

10. 10- Rodin DM, Larone D, Goldstein M. Relationship between semen cultures, leukospermia, and semen analysis in men undergoing fertility evaluation. Fertil Steril 2003; 79(3): 1555-58.

11. 11- Mancini A, Festa R, Silvestrini A, Nicolotti N, Di Donna V, et al. Hormonal regulation of total antioxidant capacity in seminal plasma. J Androl 2009; 30: 534-40.

12. 12- Lanzafame FM, Vignera SL, Vicari E, Calogero AE. Oxidative stress and medical antioxidant treatment in male infertility. Reprod Biomed 2009; 19: 638-59.

13. 13- Desai N E, Sabanegh J R, Kim T, Agarwal A. Free radical theory of aging: Implications in male infertility. Urology 2009; 75: 14-19.

14. 14- Pons-Rejraji H, Sion B, Saez F, Brugnon F, Janny L. Role of Reactive Oxygen Species (ROS) on human spermatozoa and male infertility. Gynecol Obstet Fertil 2009; 37: 529-635.

15. 15- Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol 2009; 16(5): 449-57.

16. 16- Bozhedomov VA, Gromenko DS, Ushakova IV, Toroptseva MV, Galimov SN, Alekcandrova LA, et al. Oxidative stress of spermatozoa in pathogenesis of male infertility. Urologia 2009; 2: 51-6.

17. 17- Tremellen K. Oxidative stress and male infertility: a clinical perspective. Hum Reprod Update 2008; 14(3): 243-58.

18. 18- Tempest H G, Homa ST, Routledge EJ, Garner A, Zhai XP, Griffin DK. Plants used in Chinese medicine for the treatment of male infertility possess antioxidant and anti-oestrogenic activity. Syst Biol Reprod Med 2008; 54(4-5): 185-95.

19. 19- Gallardo JM. Evaluation of antioxidant system in normal semen. Rev Invest Clin 2007; 59: 42-7.

20. 20- Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79(4): 829-43.

21. 21- Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2004; 2: 12.

22. 22- Tavilani H, Doosti D, Saeidi H, Hosseinipanah M, Hasani H. Relationship of sperm parameters with lipid peroxidation in asthenozoospermic and normozoospermic males. MJIRI 2006; 19(4): 339-44.

23. 23- Shang XJ, K Li, Z Q Ye, YG Chen, X Yu. Analysis of lipid peroxidative levels in seminal plasma of infertile men by highperformance liquid chromatography. Arch Androl 2004; 50: 411-16.

24. 24- Antoine L, Karine L, Jérôme G, Anne-Marie P. Values of sperm thiobarbituric acid-reactive substance in fertile men. Clinica Chimica Acta 2002; 325: 113-15.

25. 25- Kumar R, Venkatesh S, Kumar M, Tanwar M, Shasmsi MB, Kumar R, et al. Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men. Indian J Biochem Biophys 2009; 46(2): 172-77.

26. 26- Fraczek M, Szkutnik D, Sanocka D, Kurpisz M. Peroxidation components of sperm lipid membranes in male infertility. Ginekol Pol 2001; 72(2): 73-9.

27. 27- Yagi K. Assay for blood plasma or serum. Methods Enzymol 1984; 105: 328-31.

28. 28- Emokpae M A, udaia P O, Sadigh N M. Contribution of Bacteria Infection to Male Infertility in Nigerians. JHAS 2009; 8(1): 124-32.

29. 29- Cottle E, Mcmorrow J, Lennon B, Fawsy M, Cafferkey M, Harrison RF. Microbial contamination in an IVF-embryo transfer system. Fertil Steril 1996; 66(5): 776-80.

30. 30- Naessens A, Foulon W, Debruckre P, Devroey P, Lauwers S. Recovery of microorganisms in semen and relationship to smen evaluation. Fertil Steril 1980; 45: 101-5.

31. 31- Khalili MB, Sharifi MK. The effect of bacterial infection on the quality of human’s spermatozoa. Iranian J pub Health 2001; 30(3-4): 119-22.

32. 32- Khalili MB, Khalili MA. Relation between asymptomic urtrit with bacteriospermia in simen of fertile and infertile men. J Fer Infer 2001; 81: 21-8.

33. 33- Hillier SL, Rabe LK, Muller CH, Zarutskie P, Kuzan FB, Stenchever MA. Relationship of bacteriologic characteristics to semen indices in men attending an infertility clinic. Obstet Gynecol 1990; 75(5): 800-4.

34. 34- Balmelli T, Stamm J, Dolina-Gludici M, Peduzzi R, Piffaretti-Yanez A, Balerna M. Bacterqides ureolyticus in men consulting for infertility. Andrologia 1994; 26(1): 35-8.

35. 35- Eggert-Kruse W, Rohr G, Ströck W, Pohl S, Schwalbach B, Runnebaum B. Anaerobes in ejaculates of subfertile men. Hum Reprod Update 1995; 1(5): 462-78.

36. 36- Momoh ARM, Okome GBO, Omorogbe F IO. Association of bacteria and Chlamydia with primary infertility in males. Nigerian Annals Natual Sci 2009; 8(2): 25-9.

37. 37- Tvrda E, Knazicka Z, Bardos L, Massanyi P, Lukac N. Impact of oxidative stress on male fertility. Acta Vet Hung 2011; 59(4): 465-84.

38. 38- Holmes K K, Brger R F, Alexander E R. Acute epididymitis: etiology and therapy. Arch Androl 1979; 3: 309-16.

39. 39- Zarghami N, Khosrowbeygi A. Evaluation of Lipid Peroxidation as an Indirect Measure of Oxidative Stress in Seminal Plasma. Iran J Reproductive Med 2004; 2(1): 34-9.

40. 40- Armstrong JS, Rajasekaran M, Chamulitrat W, Gatti P, Hellstrom WJ, Sikka SC. Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radic Biol Med 1999; 26(7-8): 869-80.

41. 41- Hosseinzadeh Colagar A, Karimi F, Jorsaraei G A. Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels. Iran Red Crescent Med J 2013; 15(9): 780-85.

42. 42- Park NC, Park HJ, Lee KM, Shin DG. Free radical scavenger effect of rebamipide in sperm processing and cryopreservation. Asian J Androl 2003; 5: 195-201.

43. 43- Al-Janabi A, Abdul J, Pemaraju S, Pruthi P, Pruthi V. The role of bacterial infections on male infertility in al-anbar province of IRAQ. Bacterial Infections and Male Infertility 2014; 3(2): 23-31.

44. 44- Akbari-Asbagh A, Mostafavi E, Hamdi K, Azmodeh O, Ghasemynejad A. Relation of Serum and Semen Malondialdehyde and Total Anti-Oxidants with Sperm Parameters in Infertile Men. American J Immunology 2010; 6(3): 43-9.

45. 45- Tavilani H, Goodarzi MT, Vaisi-raygani A, Salimi S, Hassanzadeh T. Activity of antioxidant enzymes in seminal plasma and their relationship with lipid peroxidation of spermatozoa. Int Braz J Urol 2008; 34(4): 485-91

48.