Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016

mirzaee, mohsen; ,

Volume 24, Issue 11 (Feb 2017) JSSU 2017, 24(11): 913-923 | Back to browse issues page

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mirzaee M. Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016. JSSU 2017; 24 (11) :913-923
URL: http://jssu.ssu.ac.ir/article-1-3572-en.html

Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections from selective hospitals of Tehran, Boroujerd and Sanandej City in 2015-2016

Mohsen Mirzaee ^*

Abstract: (5520 Views)

Introduction: Urinary tract infection (UTI) caused by E coli is one of the most common diseases in community. Colonization of E. coli and its attachment to the uroepithelium are mediated by adhesions such as P, Fim and S fimbriae. The present study was aimed to evaluate the prevalence of fimbrial virulence genes in Escherichia coli (E. coli) isolates from the patients with urinary tract infection in Tehran, Boroujerd and Sanandaj cities, Iran.

Methods: In this descriptive cross sectional study, 150 clinical isolates of uropathogenic E.coli were collected from the patients with urinary tract infection. All bacterial isolates were identified by standard biochemical laboratory methods; the fim, pap and sfa genes were detected using the PCR and Multiplex-PCR methods.

Results: One hundred forty-five (96.66%) isolates were positive for fim gene, one hundred forty (93.33%) isolates were positive for pap gene and seven (4.66) isolates were positive for sfa gene. Whole of the isolates were possessed at least one of the three virulence genes. Six (4%) isolates were positive for all genes.

Conclusion: The findings of this study showed the high frequency of fim and P fimbriae among uropathogenic E.coli isolates from the patients with urinary tract infection. Because of the higher prevalence of UTI in the presence of these genes, detection of the genes in urine samples may help in more suspicious and rapid management of UTI.

Keywords: Escherichia coli, pap, fim, sfa genes, Urinary tract infection

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Type of Study: Original article | Subject: Microbiology
Received: 2016/01/14 | Accepted: 2017/01/7 | Published: 2017/04/16

References

1. Sarowar S, Hu OJ, Werneburg GT, Thanassi DG, Li H. The Escherichia coli P and type 1 pilus assembly chaperones PapD and FimC are monomeric in solution. J Bacteriol 2016; 198(17): 2360-69.

2. Wojnicz D, Tichaczek-Goska D, Korzekwa K, Kicia M, Hendrich AB. Study of the impact of cranberry extract on the virulence factors and biofilm formation by Enterococcus faecalis strains isolated from urinary tract infections. Int J Food Sci Nutr 2016; 67(8): 1005-16.

3. Flores-Mireles AL, Walker JN, Caparon M, Hultgren SJ. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature Rev Microbiol 2015; 13(5): 269-84.

4. K. Ejrnaes. Bacterial characteristic of importance for recurrent urinary tract infection caused by Escherichia coli. Dan Med Bull. 2011;58.

5. Mirzaee M, Owlia P, Mansouri S. Distribution of CTX-M β-lactamase genes among Escherichia coli strains isolated from patients in Iran. Lab Med 2009; 40(12): 724-7.

6. U. Jodal, S. Ahlstedt, B. Carlson, al. e. s in childhood urinary tract infection: a preliminary study. Int Arch Allergy Appl Immunol 1974; 47: 46-537.

7. Yang X, Sha K, Xu G, Tian H, Wang X, Chen S, Wang Y, et al. Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility. Int J Mol Sci 2016; 17(7): 979.

8. TA. Schalger. Urinary tract infections in children younger than 5 years of age. Pediatric Drugs 2001; 3(3): 27-219.

9. EM. Antao, LH. Wieler, C. Ewers. Adhesive threads of extraintestinal pathogenic Escherichia coli. Gut Pathog 2009; 1(1): 29.

10. JB. Kaper, JP. Nataro, HL. Mobley. Pathogenic Escherichia coli. Nat Rev Microbiol 2004; 2(2): 140.

11. JR. Johnson, AL. Stell.Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 2000; 181(1): 72-261.

12. MC. Lane, AN. Simms, HL. Mobley. Complex interplay between type 1 fimbrial expression and flagellum-mediated motility of uropathogenic Escherichia coli. J Bact 2007; 189: 5523-33.

13. Sarkar S, Roberts LW, Phan MD, Tan L, Lo AW, Peters KM, Paterson DL, Upton M, Ulett GC, Beatson SA, Totsika M. Comprehensive analysis of type 1 fimbriae regulation in fimB‐null strains from the multidrug resistant Escherichia coli ST131 clone. Mol Microbiol 2016; 101(6): 1069-87.

14. KW. Dodson, JS. Pinkner, T. Rose. Structural basis of the intraction of the pyelonephritic E.coli adhesion to its human kidney receptor Cell 2001; 105: 43-733.

15. H. Momtaz, A. Karimian, M. Maddani, F. SafapourDehkordi, R. Ranjbar, M. Sarshar, et al. Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties. Ann Clin Microbiol Antimicrob 2013; 12:8.

16. WE. Stamm, SR. Norby. Urinary tract infections: disease panorama and challenges. Stamm WE, Norrby SR Urinary tract infections: disease panorama and challenges J Infect Dis 2001;183:Suppl 1:S1-S4. 2001.

17. A. Karimian, H. Momtaz, M. Madani. Detection of uropathogenic E. coli virulence factors in patients with UTI in Iran. Islamic Azad University, Shahrekord, Iran 2012; 6(39): 6811-6.

18. SD. Manning, L. Zhang, B. Foxman, A. Spindler, P. Tallman, CF. Marrs. Prevalence of known P-fimbrial G alleles in Escherichia coli and identification of a new adhesin class. Clin Diagn Lab Immunol. 2001;8:637-40.

19. M. Bazar. Urinary tract infection. In: Mechanisms of microbeal disease. 2th ed New York Williams and Wilkins 1993: 735-43.

20. M. Riberio, Tand. Y, D. SL. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Inst Med trop Sao Paulo 2008; 50(5): 255-60.

21. P. O'Hanley, D. Low, I. Romero, D. Lark, K. Vosti, S. Falkow, et al. Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli. N Engl J Med. 1985;313(7):20-414.

22. CR. Usein, M. Damian, D. Tatu chitaiu, Capusa. C, R. Faqaras, D. Tudorache. of virulence genes in E. coli strains isolated from Romanian adult UTI cases. Molecular epidemiolgy laboratory, cantracuzinoinstitiute Bucharest, Romanian. J Cell Mol Med 2001; 5(3): 303-10.

23. Kaczmarek A., Budzynska A., Gospodarek E. Prevalence of genes encoding virulence factors among E.coli with K1 antigen & non-K1 E.coli strains. Nicolaus Copernicus University of Tourn J Med Microbiol 2012;321-326.

24. Moreno E, Andreu A, Perez T, Sabate M, Johnson JR, Prats G. Relationship between Escherichia coli strains causing urinary tract infection in women and the dominant faecal flora of the same hosts. Epidemiol and infec 2006; 134(05): 1015-23.

25. Riccabona M. Urinary tract infections in children. Current Opinions in Urology. 2003 Jan; 13(1): 59-62.

26. Matulay JT, Mlynarczyk CM, Cooper KL. Urinary Tract Infections in Women: Pathogenesis, Diagnosis, and Management. Curr Bladder Dysfunct Rep 2016; 11(1): 53-60.

27. Norgen M., Baga M., Tennent JM., Normark S.. Nucleotide sequence, regulation and functional analysis of the papC gene required for cell surface localization of Pap pili of uropathogenic Escherichia coli. Mol Microbiol 1987; 1(2): 78-169.

28. Gander RM., Tomas VL., Forland M.. Mannoseresistant hemagglutination and P receptor recognation of uropathogenic Escherichia coli isolated from adult patients. J Infect Dis 1985 1985;151(3):13-508.

29. Mehnert-key SA. Diagnosis and management of uncomplicated urinary tract infections. Am-erican family ohysician. 2005;72(3):6-451.

30. Dormanesh B., Mirnejad R., KDodaverdi dariyan E., Momtaz H., Yahaghi E., Safapour Dehkordi F., et al. Characterization and study the antibiotic resistance of Uropathogenic Escherichia coli isolated from pediatrics with pyelonephritis and cystitis in iran. Iran J Med Microbiol 2013; 7(2): 27-39.

31. Farshad S, Emamghorashi F, Amin Shahidi M. Epidemiologic evaluation of virulence genes, pap، sfa، cnf-1، hlyin E. colistrains isolated from children with urinary tract infection. Iran J Med Microbiol 2009; 2(3- 4) 31-37

32. Arisoy M., Aysev D., Ekim M., Özel D., Kose SK., Özsoy ED., et al. Detection of virulence factors of Escherichia coli from children by multiplex polymerase chain reaction. Int J Clin Pract 2006; 60(2): 3-170.

33. Johnson JR., Kuskowski MA., GajewskiA. al. e. Extended virulence genotypes and phylogenetic background of Escherichia coli isolates from patients with cystitis, pyelonephritis, or prostatitis. J Infect Dis 2005; 19:46-50.