Evaluation the effect of hydro-alcoholic extract of GlycyrrhizaGlabra rhizome on the isolated colon contractions of male rats

Ghayedi , N; Bahaoddini , A; Khoshnam , SE; Gholampour, F; Khosravi, AR; Moein, MR

Volume 24, Issue 7 (Oct 2016) JSSU 2016, 24(7): 576-586 | Back to browse issues page

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Ghayedi N, Bahaoddini A, Khoshnam S, Gholampour F, Khosravi A, Moein M. Evaluation the effect of hydro-alcoholic extract of GlycyrrhizaGlabra rhizome on the isolated colon contractions of male rats. JSSU 2016; 24 (7) :576-586
URL: http://jssu.ssu.ac.ir/article-1-3355-en.html

Evaluation the effect of hydro-alcoholic extract of GlycyrrhizaGlabra rhizome on the isolated colon contractions of male rats

N Ghayedi

, A Bahaoddini

, SE Khoshnam ^*

, F Gholampour

, AR Khosravi

, MR Moein

Abstract: (6379 Views)

Introduction:The licorice (Glycyrrhizaglabra) rhizome has been widely used in traditional medicine for treatment of gastrointestinal diseases such as gastric ulcer and relieve intestinal spasms. In the present study, the effect of hydro-alcoholic extract of licoricerhizome on mechanical activity of isolated colon of male rats has been studied.

Methods: Adult male rats were anesthetized by ethyl ether, their abdomen opened, and colon tissues were removed and divided into 1 cm segments. The segments were connected to a force transducer longitudinally and inserted to an organ bathe contained oxygenated Tyrode solution (37 °C, pH=7.4). Their mechanical activity of ileum was recorded by power lab AD instrument in basal condition, and after administration of L-NAME (10^-4M), acetylcholine (4×10^-5M) and Atropine (10^-5M) drugs in the presence and absence of licorice rhizome extract were recorded (0.036mg/ml). Also, the mechanical activity of control group segments were recorded at the same condition with extract solvent (ethanol %70).

Results: A significant decrease in mechanical activity of the isolated colon occurred after administration of hydro-alcoholic extract of licorice rhizome compared to the control group (p≤0.05). Also, a significant decrease was seen in mechanical activity occurredin the co-administration of extract and acetylcholine compared to the control group. The mechanical activity of tissue was not significantly changed in the presence of Atropine and extract between experimental and control groups. The mechanical activity of ileum tissue was not significantly changed in the co-administration of L-NAME and extract between experimental and control groups.

Conclusion: We can conclude that hydro-alcoholic extract of licorice has modifying effect on colon motility, and this activity may be occurred independently in the nitrergic and cholinergic systems.

Keywords: GlycyrrhizaGlabra, Colon, Nitric Oxide, Cholinergic

Full-Text [PDF 767 kb] (2524 Downloads)

Type of Study: Original article | Subject: Physiology
Received: 2015/08/12 | Accepted: 2016/06/5 | Published: 2016/11/22

References

1. Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nature reviews Drug discovery 2005; 4(3): 206-20.

2. Khoshnam SE, Farzaneh M, valipour M, Bahaoddini A, valipour A. Review of the phytochemical, pharmacological and physiological properties of Licorice (Glycyrrhizaglabra). J Clinic Excellence 2015; 4(1): 71-56.

4. Khoshnam SE, Bahaoddini A, Vatanparast J, Golampour F, Khosravi A. The Effect of Hydro-alcoholic Extract of Glycyrrhizaglabra on Electrocardiogram and Its Interaction with Cholinergic System of Male Wistar Rats. Armaghanedanesh 2015; 20 (4): 287-297.

5. Khayyal MT, El-Ghazaly MA, Kenawy SA, Seif-El-Nasr M, Mahran LG, Kafafi Y, et al. Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination. Arzneimittel-forschung 2000; 51(7): 545-53.

7. 5- Amani M, Hosein RS-GNM, Kashani AM. Optimal Extraction of Glycyrrhetinic Acid Froml icorice Root. J Food Tech 2005; 3(4): 576-80.

8. Saxena S. Glycyrrhiza glabra: medicine over the millennium. Nat Prod Rad 2005; 4(5): 358-67.

9. Li W, Asada Y, Yoshikawa T. Flavonoid constituents from Glycyrrhiza glabra hairy root cultures. Phytochemist 2000; 55(5): 447-56.

10. Khayyal MT, El-Ghazaly MA, Kenawy SA, Seif-El-Nasr M, Mahran LG, Kafafi Y, et al. Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination. Arzneimittel-forschung. 2000; 51(7): 545-53.

11. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S, Nomura T. Anti-Helicobacter pylori flavonoids from licorice extract. Life Sci 2002; 71(12): 1449-63.

12. Aly AM, Al-Alousi L, Salem HA. Licorice: a possible anti-inflammatory and anti-ulcer drug. AAPS Pharm Sci Tech. 2005; 6(1): 74-82.

13. Chen G, Zhu L, Liu Y, Zhou Q, Chen H, Yang J. Isoliquiritigenin, a flavonoid from licorice, plays a dual role in regulating gastrointestinal motility in vitro and in vivo. Phytotherapy Res 2009; 23(4): 498-506.

14. Huang W-F, Ouyang S, Li S-Y, Lin Y-F, Ouyang H, Zhang H, et al. Effect of quercetin on colon contractility and L-type Ca (2+) channels in colon smooth muscle of guinea-pig. Sheng li xue bao:[Acta physiologica Sinica] 2009; 61(6): 567-76.

15. Yamaguchi T, Kamada K, Dayton C, Gaskin FS, Yusof M, Yoshikawa T, et al. Role of eNOS-derived NO in the postischemic anti-inflammatory effects of antecedent ethanol ingestion in murine small intestine. Am J Physiol-Heart Circulatory Physiol 2007; 292(3): 1435-42.

16. 14- Sigurjonsdottir H, Franzson L, Manhem K, Ragnarsson J, Sigurdsson G, Wallerstedt S. Liquorice-induced rise in blood pressure: a linear dose-response relationship. J Human Hypertension 2001; 15(8): 549-52.

18. 15- Matsui M, Motomura D, Fujikawa T, Jiang J, Takahashi S-i, Manabe T, et al. Mice lacking M2 and M3 muscarinic acetylcholine receptors are devoid of cholinergic smooth muscle contractions but still viable. The J Neuroscience 2002; 22(24): 10627-32.

19. 16- Iino S, Nojyo Y. Muscarinic M 2 acetylcholine receptor distribution in the guinea-pig gastrointestinal tract. Neuroscience 2006; 138(2): 549-59.

20. 17- Chen G, Zhu L, Liu Y, Zhou Q, Chen H, Yang J. Isoliquiritigenin, a flavonoid from licorice, plays a dual role in regulating gastrointestinal motility in vitro and in vivo. Phytotherapy Res 2009; 23: 498-506.

21. 18- Thomford NE, Dzobo K, Chopera D, Wonkam A, Skelton M, Blackhurst D, et al. Pharmacogenomics implications of using herbal medicinal plants on african populations in health transition. Pharmaceuticals 2015; 8(3): 637-63.

22. 19- Khoshnam SE, Bahaoddini A. The effect of hydro-alcoholic extract of Glycyrrhiza glabra on the cardiovascular system of male rats with normal blood pressure and its interaction with cholinergic and adrenergic systems. Physiol Pharmacol 2013; 17(3): 349-58.

23. 20- Khoshnazar SM, Bahaoddini HN. Effect of Alcoholic Extract of Licorice (Glycyrrhiza glabra l.) Rhizome on Isolated Duodenum Motility in Male Rats and its Interference with Cholinergic, Nitrergic, and Adrenergic Systems. Bull Env Pharmacol Life Sci 2013; 2(12): 173-7.

24. 21- Gharib S, Bahaoddini A, Vatanparast J, Moein M. Effect of alcoholic extract of ginger (Zingiber Officinale Roscoe) on mechanical activity of isolated jejunum of male rat. Physiol Pharmacol 2014; 18(4): 406-15.

25. 22- Bahaoddini A, Ketabi MA, Gholampour F, Mirkhanni H. Effect of prolonged exposure to low-frequency electromagnetic fields on the interaction of nitrergic and cholinergic systems in the isolated rat trachea. Physiology Pharmacol 2011; 12(3): 55-63.

26. 23- Di Carlo G, Autore G, Izzo A. A, Maiolino P, Mascolo N, Viola p, Diurno M. V, Capasso F. (1993). Inhibition of intestinal motility and secretion by flavonoids in mice and rats: structure-activity relationship. J Pharm Pharmacol 1993, 45: 1054-1059.

27. 24- Sato Y, He J-X, Nagai H, Tani T, Akao T. Isoliquiritigenin, one of the antispasmodic principles

28. of Glycyrrhiza ularensis roots, acts in the lower part of intestine. Biologic Pharmaceutic Bulletin 2007; 30(1): 145-9.

30. 25- mohamad kazem Gharib-Naseri mA, zahra Gharib-Naseri. Antispasmodic Effect of hydroalcoholic leaf extract of licorice ileum contraction in rat. Shahrekord J Med Sci 2008; 9(3): 1-9.

31. 26- Liu B, Yang J, Wen Q, Ying Li. Isoliquirtigenin: a flavonoid from licorice, relaxes guinea-pig tracheal smooth muscle invitro and invivo: Role of cGMP/PKG pathway. Eur J Pharmacol 2008; 587: 257-266.

32. 27- Nagai H, Yamamoto Y, Sato Y, Akao T, Tani T. Pharmaceutical evaluation of cultivated Glycyrrhiza uralensis roots in comparison of their antispasmodic activity and glycycoumarin contents with those of licorice. Biologic Pharmaceutic Bulletin 2006; 29 (12): 2442-45.