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Introduction: Blood glucose is high in diabetic patients. It is taken from blood by two separate pathways: Insulin-dependent pathway of phosphoinositide 3 kinase (PI3K) and insulin-independent pathway AMPK (AMP-Activated protein kinase). The first pathway is impaired in type 2 diabetic patients, but the second pathway is active. On the other hand, curcuma longa extract containing a high percentage curcumin has Anti-diabetic effects, but its exact mechanism is not clear. Therefore, this study intends to investigate the effect of curcumin on the activation of the critical pathway AMPK in C2C12 cells. Methods: C2C12 cells, after growth and differentiation to muscle myoblast, were exposed to 40 µM concentrations of curcumin (test group) and 0.1%DMSO (control) for one hour. Then cells were collected, lysed and p-AMPK and p-ACC proteins were identified by Western blotting technique. Data were normalized with the β-Actin internal control and each band was calculated as arbitrary number compared with the control. Significant results were determined by using SPSS statistical software and student t-test. Results: Phosphorylation of enzymes, that indicates their activation, significantly increased for p-AMPK (23±1.6) and for p-ACC (31.7±2) in presence of curcumin Compared to the control (3±0.5 1.7±0.4) (p-value <0.05). Conclusion: The results demonstrated that anti-diabetic effects of curcumin could be done through the activation of AMPK / ACC.

Keywords: AMPK (AMP-activated protein kinase); C2C12; Curcumin

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