Introduction: Toothpaste should have the most plaque removal efficacy with the least abrasiveness. The aim of this study was to evaluate enamel abrasion induced by four toothpaste types. Methods: In this in vitro experimental study, 24 dental samples were divided into four groups of six. The initial surface roughness was measured with the roughness measuring device. Regarding abrasion test with Daroghar3, Nasim, Crest7 and Crest sensitivity toothpastes, samples were located in V8cross brushing machine. After washing and drying, the secondary surface roughness was again measured with the roughness measuring device. Average wear of samples was analyzed by means of ONEWAY ANOVA. Results: The study results indicated no significant difference among the four types of toothpaste (p=0.855). The toothpastes were ranked in regard with average roughness changes as fallows: Nasim, Crest 7, Crest sensitivity and Daroghar3. Conclusion: The toothpastes investigated in this study did not reveal any difference in terms of wear index.