Volume 13, Issue 4 (Atumn 2005)
JSSU 2005, 13(4): 58-63 |
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Yaftiyan M, Salehnia M, Pourfatollah A. Effect of Co-Culturing of Mice Liver Cells and Embryonic Carcinomatous Stem Cells on the Rate of Differentiation to Hematopoietic Cells. JSSU 2005; 13 (4) :58-63
URL: http://jssu.ssu.ac.ir/article-1-703-en.html
URL: http://jssu.ssu.ac.ir/article-1-703-en.html
Abstract: (7035 Views)
Introduction: Considering the importance of co-culture in differentiation of embryonic stem cells, the aim of
this study was evaluation of the effect of co-culturing fetal liver stroma cells with P19 cells on the line of
differentiation.
Materials and Methods: For this purpose, P19 cells were cultured directly in semisolid medium. These cells
proliferated and primarily differentiated to colonies know as embryoid bodies (EBs) after 8-12 days.
The Ebs cells were trypsinized and dissociated to single or double cells.
Then these cells were co-cultured on the mouse fetal liver feeder layer in the absence of exogenous factors.
After 14-18 days, the colonies were studied morphologically by benzidine and giemsa staining and also
counted under invert microscope.
Results: The percentages of benzidine positive (or erythroid) and negative colonies were 94% and 6%
respectively and also the cells of colonies were studied by Giemsa staining. Results showed that they were
myeloid or lymphoid type cells.
Thus, the results show that in the presence of mouse fetal liver feeder layer, the number of erythroid colonies
was increased.
Conclusions: Therefore, this technique may be effective for differentiation of stem cells from different
sources into hematopoietic cells and can be used in future for human cell therapy.
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