دوره 13، شماره 4 - ( پاییز 1384 )                   جلد 13 شماره 4 صفحات 58-63 | برگشت به فهرست نسخه ها

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yaftiyan M, Salehnia M, Pourfatollah A. Effect of Co-Culturing of Mice Liver Cells and Embryonic Carcinomatous Stem Cells on the Rate of Differentiation to Hematopoietic Cells. JSSU. 2005; 13 (4) :58-63
URL: http://jssu.ssu.ac.ir/article-1-703-fa.html
یافتیان مرتضی، صالح نیا مژده، پور فتح اله علی اکبر. تاثیر هم کشتی سلول های کبد جنین موش و سلول های بنیادی کارسینومای جنینی در القاء تمایز آنها به کلنی های خونساز. مجله علمي پژوهشي دانشگاه علوم پزشكي شهید صدوقی يزد. 1384; 13 (4) :58-63

URL: http://jssu.ssu.ac.ir/article-1-703-fa.html


چکیده:   (5711 مشاهده)
Introduction: Considering the importance of co-culture in differentiation of embryonic stem cells, the aim of this study was evaluation of the effect of co-culturing fetal liver stroma cells with P19 cells on the line of differentiation. Materials and Methods: For this purpose, P19 cells were cultured directly in semisolid medium. These cells proliferated and primarily differentiated to colonies know as embryoid bodies (EBs) after 8-12 days. The Ebs cells were trypsinized and dissociated to single or double cells. Then these cells were co-cultured on the mouse fetal liver feeder layer in the absence of exogenous factors. After 14-18 days, the colonies were studied morphologically by benzidine and giemsa staining and also counted under invert microscope. Results: The percentages of benzidine positive (or erythroid) and negative colonies were 94% and 6% respectively and also the cells of colonies were studied by Giemsa staining. Results showed that they were myeloid or lymphoid type cells. Thus, the results show that in the presence of mouse fetal liver feeder layer, the number of erythroid colonies was increased. Conclusions: Therefore, this technique may be effective for differentiation of stem cells from different sources into hematopoietic cells and can be used in future for human cell therapy.
متن کامل [PDF 183 kb]   (2000 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: 1388/11/5 | انتشار: 1384/7/23

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
CAPTCHA

ارسال پیام به نویسنده مسئول


کلیه حقوق این وب سایت متعلق به ماهنامه علمی پ‍ژوهشی دانشگاه علوم پزشکی شهید صدوقی یزد می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2021 All Rights Reserved | SSU_Journals

Designed & Developed by : Yektaweb