Effects of Cantharellus Cibarius Hydro-Alcoholic Extract on NT3, NGF Gene Expression after Sciatic Nerve Compression in Rats

Ajami , AR; Tehranipour , M; Nezhad Shahrokh Abadi , KH; Zokaee , M

Volume 24, Issue 8 (Nov 2016) JSSU 2016, 24(8): 679-689 | Back to browse issues page

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Ajami A, Tehranipour M, Nezhad Shahrokh Abadi K, Zokaee M. Effects of Cantharellus Cibarius Hydro-Alcoholic Extract on NT3, NGF Gene Expression after Sciatic Nerve Compression in Rats. JSSU 2016; 24 (8) :679-689
URL: http://jssu.ssu.ac.ir/article-1-3803-en.html

Effects of Cantharellus Cibarius Hydro-Alcoholic Extract on NT3, NGF Gene Expression after Sciatic Nerve Compression in Rats

AR Ajami

, M Tehranipour ^*

, KH Nezhad Shahrokh Abadi

, M Zokaee

Abstract: (5955 Views)

Introduction: The expression of neurotrophic factors, which cause increasing of the survival and regeneration of neurons, is changed in response to nerve injury. Cantharellus cibarius has antioxidant and anti-inflammatory effects. So, the aim of present study was to determine the effects of hydro-alcoholic extract of Cantharellus cibarius on expression level of NT3 gene after sciatic nerve compression in rats.

Methods: First the hydro-alcoholic extract of Cantharellus cibarius was prepared by the Soxhlet method. In this study, 36 Wistar male rats , 250-300 gr, were randomly divided into 3 groups consisted of 12 rats in each group. They were control, compression (1, 7, 14 and 28 days) and experimental (1, 7, 14 and 28 days) groups. Experimental groups were treated by 75 mg / kg of hydro-alcoholic extract of Cantharellus cibarius and to induce the stress in control group, saline serum was injected. In compression and experimental groups, the sciatic nerve of right leg was compressed for 60 seconds. The first injection of extract in the experimental group was performed intraperitoneally and immediately after the compression and the second one was injected 7 days later. Then, the sampling was performed of lumbar spinal cord on 1, 7, 14 and 28 days in compression and experimental groups and the total RNA was extracted from the spinal cord segments, cDNA was synthesized and after that the alteration of gene expression of NT3 and NGF samples was studied real time PCR method and Data were analyzed by Tukey test and SPSS 16 softwere.

Results: The expression of NT3 and NGF showed significant increase in compression group compared to the control group, (p<0.05). Also, it is shown significant increase of expression of NT3 and NGF in the experimental group compared to the compression group, (p<0.05).

Conclusion: According to these findings, hydro alcoholic extract of Cantharellus cibarius has an anti oxidant and anti-inflammatory effects that increase the regeneration process expression of NT3 and NGF in the experimental group was increase compared with the compression group.

Keywords: Cantharellus Cibarius, Regeneration, Degeneration, NT3 Gene, NGF Gene

Full-Text [PDF 832 kb] (1665 Downloads)

Type of Study: Original article | Subject: Physiology
Received: 2016/07/5 | Accepted: 2016/10/22 | Published: 2017/02/1

References

1. Behnam-Rasouli M, Nikravesh MR, Mhadavi-Shahri N, Tehranipour M. Post-operative time effects after sciatic nerve crush on the number of alpha motoneurons, using a sterological counting method (disector). Iran Biomed J 2000; 4(1): 45-9.

2. DeVivo M. Epidemiology of Spinal Cord Injury. In: Lin V, editor. Spinal cord medicine: principles and practice. New York: Demos Medical Publishing; 2003. p. 79-86.

3. Nathan C. “Points of control in inflammation.” Nature 2002; 420(6917): 846-52.

4. Cook-Mills JM, Deem TL. “Active participation of endothelial cells in inflammation.” J Leukocyte Biology 2005; 77(4): 487-95.

5. Abdel moneim AE. The neuroprotective effectc of purslane(portulaca oleracea) on rotenon-induced biochemical changes and apoptosis in brain of rat. CNS Neuro Disord Drug Targets 2013; 12(6): 830-41.

6. Gu JF, Zheng ZY, Yuan JR, Zhao BJ, Wang CF, Zhang L, et al. Comparison on hypoglycemic and antioxidant activites of the fresh and drive Portulaca olearacea L. in insulin resistant HepG2 cells and streptozotocin-induced c57BL/6J diabetic mice. J Ethnnopharmacol 2015; 161: 214-23.

7. Cicchetti E, Chaintreau A. Comparison of extraction techniques and modeling of accelerated solvent extraction for the authentication of natural vanilla flavors. J Sep Sci 2009; 32(11): 1957-64.

8. Tehranipour M, Ghamyari T. The effects of root aquatic extract of Salvia staminea on neuronal density of alpha motoneurons in spinal cord anterior horn after sciatic nerve compression in rats. J Biol Sci 2010; 10(1): 48-52.

9. Moro C, Palacios I, Lozano M, D’Arrigo M, Guillamón E, Villares A, et al. “Anti-inflammatory activity of methanolic extracts from edible mushrooms in LPS activated RAW 264.7 macrophages.” Food Chemistry 2012; 130(2): 350-55.

10. Ferrer I, Planas AM. Signaling of cell death and cell survival following focal cerebral ischemia: life and death struggle in the penumbra. J Neuropathol Exp Neurol 2003; 62(4): 329-39.

11. Barros L, Venturini BA, Baptista P, Estevinho LM, Ferreira ICFR. Chemical composition and biological properties of Portuguese wild mushroom: A comprehensive study. J Agric Food Chem 2008; 56(10): 3856-862.

12. Pan H, Hu XZ, Jacobowitz DM, Chen C, McDonough J, Van Shura K, Lyman M, Marini AM. Alpha-linolenic acid is a potent neuroprotective agent against soman-induced neuropathology. Neurotoxicology 2012; 33(5): 1219-29.

13. Jamalpoor Z, Asgari AR, Nourani MR. Skeletal muscle tissue engineering: Present and future. J Milit Med. 2012; 14(2): 77-84.

14. Linshan Fu, Doreswamy V, Prakash R. The biochemical pathways of central nervous system neural degeneration in niacin deficiency. Neural Regen Res 2014; 9(16): 1509-513.

15. Sumathi T, Christinal J. Neuroprotective effect of Portulaca oleracea Ethanolic Extract Ameliorates Methylmercury Induced congnitive Dysfunction and Oxidative stress in cerebellum and cortex of Rat Brain. Bio Elem Res 2015; 172(1): 155-65.

16. Kasthuri BM, Ammu KR, Nagaraja H. Protective Mechanisms of Flavonoids in Parkinson’s Disease. Oxid Med Cell Longev 2015.