Comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression after a period of high fat diet in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats

Rahmaty, Saeed; Gaeini, Abass Ali; Choobineh, Siross; Dolatshahi, Mojtaba

Volume 26, Issue 2 (May 2018) JSSU 2018, 26(2): 111-125 | Back to browse issues page

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Rahmaty S, Gaeini A A, Choobineh S, Dolatshahi M. Comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression after a period of high fat diet in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats. JSSU 2018; 26 (2) :111-125
URL: http://jssu.ssu.ac.ir/article-1-4606-en.html

Comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression after a period of high fat diet in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats

Saeed Rahmaty ^*

, Abass Ali Gaeini

, Siross Choobineh

, Mojtaba Dolatshahi

Abstract: (3743 Views)

Introdution: Obesity is a systemic disorder that disruption the regulation of the effective proteins; in fat metabolism (Desnurtin, FNDC5 it leads to resistance to insulin and Diabetes. The aim of this study was comparing the 6 weeks of high-intensity interval and continuous training with standard diet on desnutrin and FNDC5 genes expression in subcutaneous adipose tissue and the quadriceps muscle tissue of obese male rats.
Methods: This study was fundamental, and its method was experimental. 40 Wistar rats were compared after 12 weeks of high-fat diet (n=30) and standard diet (n=10) in order to ensure obesity. Then, 30 obese rats were divided randomly into 3 groups: control (n=10), continuous training (n=10), and high-intensity interval training groups (n=10). Continues and high-intensity interval exercise trainings were conducted according to the principle of overload for 6 weeks and 6 sessions per week. Extraction and gene expression level desnutrin in subcutaneous adipose tissue, desnutrin, and FNDC5 in quadriceps muscle tissue was assessed using real time-PCR method. The one-way ANOVA test and Tukey's post hoc test were used to compare inter group changes.
Results: The study showed that the difference between high-intensity interval and continuous training on desnutrin gene expression subcutaneous adipose tissue was significant (P=0.011), but there was no significant difference in desnutrin (P=0.275) and FNDC5 genes expression (P=0.981) between them in the quadriceps muscle tissue.
Conclusion: For people who suffer from obesity, and for counteracting with the destructive effects of obesity on fat metabolism factors, including desnutrin and FNDC5, continuous training can be an effective method. However, for weight loss, high intensity interval training is the best method

Keywords: Desnutrin, FNDC5, High-intensity interval training, Continuous training.

Full-Text [PDF 1075 kb] (1554 Downloads)

Received: 2018/06/20 | Accepted: 2018/06/20 | Published: 2018/06/20

References

1. 1-Bweir S, Al-Jarrah M, Almalty AM, Maayah M, Smirnova IV, Novikova L, et al. Resistance exercise training lowers HbA1c more than aerobic training in adults with type 2 diabetes. Diabetol Metab Syndr 2009; 1(1): 27.

2. 1- Knapp M, Gorski J. The skeletal and heart muscle triacylglycerol lipolysis revisited. J physiol pharmacol: an official J Polish Physiological Soc 2017; 68(1): 3-11.

3. 2- Villena JA, Roy S, Sarkadi-Nagy E, Kim KH, Sul HS. Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: ectopic expression of desnutrin increases triglyceride hydrolysis. J biol chem 2004; 279(45): 47066-75.

4. 3- Ahmadian M, Abbott MJ, Tang T, Hudak CS, Kim Y, Bruss M, et al. Desnutrin/ATGL is regulated by AMPK and is required for a brown adipose phenotype. Cell metabolism 2011; 13(6): 739-48.

5. 4- Larsen S, Danielsen JH, Sondergard SD, Sogaard D, Vigelsoe A, Dybboe R, et al. The effect of high-intensity training on mitochondrial fat oxidation in skeletal muscle and subcutaneous adipose tissue. Scand J Med Sci Sport 2015; 25(1): 59-69.

6. 5- Morton TL, Galior K, McGrath C, Wu X, Uzer G, Uzer GB, et al. Exercise Increases and Browns Muscle Lipid in High-Fat Diet-Fed Mice. Front endocrinol 2016; 7: 80.

7. 6- Erickson HP. Irisin and FNDC5 in retrospect: An exercise hormone or a transmembrane receptor? Adipocyte 2013; 2(4): 289-93.

8. 7- Teufel A, Malik N, Mukhopadhyay M, Westphal H. Frcp1 and Frcp2, two novel fibronectin type III repeat containing genes. Gene 2002; 297(1-2): 79-83.

9. 8- Lecker SH ZA, Cao P, Arena R, Allsup K, Daniels KM, Joseph J, et al. Expression of the irisin precursor fndc5 in skeletal muscle correlates with aerobic exercise performance in patients with heart failure. Circ Heart Fail 2012; 5(6): 812-8.

10. 9- Panati K, Suneetha Y, Narala VR. Irisin/FNDC5-An updated review. Eur Rev for med pharmacol Sci 2016; 20(4): 689-97.

11. 10- Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012; 481(7382): 463-8.

12. 11- Seale P, Conroe HM, Estall J, Kajimura S, Frontini A, Ishibashi J, et al. Prdm16 determines the thermogenic program of subcutaneous white adipose tissue in mice. J clinical invest 2011; 121(1): 96-105.

13. 12- Shanshan Gao FL, Huimin Li, Yibing Huang, Yu Liu, Yuxin Chen. Effects and Molecular Mechanism of GSTIrisin on Lipolysis and Autocrine Function in T3-L1 Adipocytes. J pone0147480 2016; 11.

14. 13- Tumminello N. Strength Training for Fat Loss. 2014. pp. 1-5.

15. 14- Hoshino D, Yoshida Y, Kitaoka Y, Hatta H, Bonen A. High-intensity interval training increases intrinsic rates of mitochondrial fatty acid oxidation in rat red and white skeletal muscle. Appl Physiol Nutr Metab 2013; 38(3): 326-33.

16. 15- Zhang H, Tong TK, Qiu W, Zhang X, Zhou S, Liu Y, et al. Comparable Effects of High-Intensity Interval Training and Prolonged Continuous Exercise Training on Abdominal Visceral Fat Reduction in Obese Young Women. J Diabetes Res 2017; 2017: 5071740.

17. 16- Malcolm eaton cg, julianne barry, adeel dafdar, david bishop, jonathan p. little impact of a single bout of high-intensity interval exercise and short-term interval training on interleukin-6,FNDC5, and METRNL mRNA experssion in human skeletal muscle. J sport Health Sci 2016: 1-6.

18. 17- Bae JY, Woo J, Roh HT, Lee YH, Ko K, Kang S, et al. The effects of detraining and training on adipose tissue lipid droplet in obese mice after chronic high-fat diet. Lipids Health Dis 2017; 16(1): 13.

19. 18- Han YJ. Effects of aerobic exercise on the expressions of skeletal muscle fiber type-related genes and FNDC-5 in high-fat diet-induced obese rats. Interl J Applied Sport Sci 2017; 29(1): 42-53.

20. 19- Fabbiano S, Suarez-Zamorano N, Rigo D, Veyrat-Durebex C, Stevanovic Dokic A, Colin DJ, et al. Caloric Restriction Leads to Browning of White Adipose Tissue through Type 2 Immune Signaling. Cell metabol 2016; 24(3): 434-46.

21. 20- Menshikova EV, Ritov VB, Dube JJ, Amati F, Stefanovic-Racic M, Toledo FGS, et al. Calorie Restriction-induced Weight Loss and Exercise Have Differential Effects on Skeletal Muscle Mitochondria Despite Similar Effects on Insulin Sensitivity. J gerontology Series A, Biolog Sci medical Sci 2017; 73(1): 81-7.

22. 21- Alsted TJ, Nybo L, Schweiger M, Fledelius C, Jacobsen P, Zimmermann R, et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training. Am J physiol Endocrinol Metabol 2009; 296(3): 445-53.

23. 22- Chen N, Li Q, Liu J, Jia S. Irisin, an exercise-induced myokine as a metabolic regulator: an updated narrative review. Diabetes/metabolism Res Rev 2016; 32(1): 51-9.

24. 23- Pekkala S, Wiklund PK, Hulmi JJ, Ahtiainen JP, Horttanainen M, Pollanen E, et al. Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health?. J phys 2013; 591(21): 5393-400.

25. 24- Storlien LH, James DE, Burleigh KM, Chisholm DJ, Kraegen EW. Fat feeding causes widespread in vivo insulin resistance, decreased energy expenditure, and obesity in rats. Am J phys 1986; 251(5): 576-83.

26. 25- Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiology Behav 2015; 147: 78-83. [Persion]

27. 26- Ogasawara J, Sakurai T, Kizaki T, Ishibashi Y, Izawa T, Sumitani Y, et al. Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes. PloS One 2012; 7(7): 40876.

28. 27- Yao-Borengasser A, Varma V, Coker RH, Ranganathan G, Phanavanh B, Rasouli N, et al. Adipose triglyceride lipase expression in human adipose tissue and muscle. Role in insulin resistance and response to training and pioglitazone. Metabolism 2011; 60(7): 1012-20.

29. 28- Kim SJ, Tang T, Abbott M, Viscarra JA, Wang Y, Sul HS. AMPK Phosphorylates Desnutrin/ATGL and Hormone-Sensitive Lipase To Regulate Lipolysis and Fatty Acid Oxidation within Adipose Tissue. Molecular cellular biol 2016; 36(14): 1961-76.

30. 29- Petridou A, Chatzinikolaou A, Avloniti A, Jamurtas A, Loules G, Papassotiriou I, et al. Increased Triacylglycerol Lipase Activity in Adipose Tissue of Lean and Obese Men During Endurance Exercise. J clin endocrinology metab 2017; 102(11): 3945-3952.

31. 30- Turnbull PC, Longo AB, Ramos SV, Roy BD, Ward WE, Peters SJ. Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles. Am J physio Regulatory, integrative comparative phys 2016; 310(2): 125-33.

32. 31- Louche K, Badin PM, Montastier E, Laurens C, Bourlier V, de Glisezinski I, et al. Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects. J clinic endocrinol metabol 2013; 98(12): 4863-71.

33. 32- Gaidhu MP, Anthony NM, Patel P, Hawke TJ, Ceddia RB. Dysregulation of lipolysis and lipid metabolism in visceral and subcutaneous adipocytes by high-fat diet: role of ATGL, HSL, and AMPK. American J phys Cell phys 2010; 298(4): 961-71.

34. 33- Shepherd SO, Cocks M, Tipton KD, Witard OC, Ranasinghe AM, Barker TA, et al. Resistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary males. Exp physiol 2014; 99(6): 894-908.

35. 34- Azuma K, Osawa Y, Tabata SH, Katsukawa F, Ishida H, Oguma Y, et al. Decrease in regional body fat after long-term high-intensity interval training. J Physic Fitness Sport Med 2017; 16: 103-10

36. 35- Huh JY, Panagiotou G, Mougios V, Brinkoetter M, Vamvini MT, Schneider BE, et al. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism: clinical experimental 2012; 61(12): 1725-38.

37. 36- Park KH, Zaichenko L, Brinkoetter M, Thakkar B, Sahin-Efe A, Joung KE, et al. Circulating irisin in relation to insulin resistance and the metabolic syndrome. J clinic endocrinology metabol 2013; 98(12): 4899-907.

38. 37- Bonfante IL, Chacon-Mikahil MP, Brunelli DT, Gaspari AF, Duft RG, Lopes WA, et al. Combined training, FNDC5/irisin levels and metabolic markers in obese men: A randomised controlled trial. Eur J sport sci 2017; 17(5): 629-37.

39. 38- Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, et al. The effects of acute and chronic exercise on PGC-1alpha, irisin and browning of subcutaneous adipose tissue in humans. FEBS J 2014; 281(3): 739-49.