Volume 25, Issue 9 (Nov 2017)                   JSSU 2017, 25(9): 728-735 | Back to browse issues page

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Mottaghi M, Safinejad K, Mohammad Asghari H. Evaluation of Mutagenicity and Carcinogenicity of Opium Using the Ames Test . JSSU. 2017; 25 (9) :728-735
URL: http://jssu.ssu.ac.ir/article-1-4265-en.html
Abstract:   (707 Views)
Introduction: Cancer is one of the most common diseases in the world. Due to the carcinogenic combinations of drug, there is a risk of mutagenic substances in consumers. The aim of this study was to investigate the mutagenicity of opium by the Ames test.
Methods: Firstly, the purity of the Salmonella typhimurium strain TA100 was confirmed in terms of purity of mutagenic properties. Then, samples of opium were prepared separately in different volumes and by various repetitions and were added to the culture medium containing TA100 fresh night culture, after that subsequently were compared with negative control (distilled water+ TA100) samples. In that case, colonies were evaluated in terms of mutagenicity in three stages: without the buffer microsomal rat liver (S9) -without pre incubation, with S9– with pre incubation and with S9- without pre incubation. The colonies were evaluated by counting revertant TA100 colonies number. The results were analyzed using ANOVA and Schefe's post hoc test to determine and prioritize the difference in mean of groups based on opium dilution.
Results: Results of data analysis and counting of revertant coloniesshowed that opium had mutagenic effect in over 4% dilution. There were significant differences in dilution and repetition of without S9- without pre incubation, with S9- with pre incubation and with S9 without pre incubation compared to the negative controls.
Conclusion: The results of this research showed that opium is fully and on all volumes has mutagenic effect. Therefore, we recommend taking these drugs should be avoided.
Full-Text [PDF 669 kb]   (183 Downloads)    
Type of Study: Original article | Subject: Microbiology
Received: 2017/07/24 | Accepted: 2017/10/14 | Published: 2018/01/8

2. Wessner DR, Maiorano PC, Kenyon J, Pillsbury R, Campbell AM. (Spot- over- lay Ames test of potential mutagens, See in- formation in: http://www.zoo.utoronto.ca/able 2000;1-16.
3. Bertman G. Katzong. Basic and clinical pharmacology. Mc Graw- HillCompanies Inc 2009; 6th ed. P: 531-68.
4. Kaplan, Sadock. Comprehensive text book of psychiatry. Eighth ed. Philadelphia. Vol:2. USA: lippincot Williams, Wilkins 2010; 1265-91.
5. Shahramian I, Kohan F, Moradi A. Opioids in pediatric. Opioid history, first Ed, Golban press 2004:1-3, 80.
6. Shirazi EM. Efionieh Thesis.1nd Ed. Tehran: Almaa 2009; 134,142,146,149. [Persian]
7. Hallinan R, Byrne A, Agho K, McMahon C, Tynan P, Attia J. Erectile dysfunction in men receiving methadone and buprenorphine maintenance treatment. J sexual med 2008; 5: 684-92.
8. Maron DM, Ames BN. Revised methods for the Salmonella mutagenicity test. Mut. Res 1983; 113: 173- 212.
9. Emtiazjo M. Mutagenic and carcinogenic effects of three additives compounds to crude oil of Persian Gulf by salmonella mutagenicity test [dissertation].Tehran Uni; 2007.
10. The study of mutagenic and carcinogenic effect of heavy and light polyethylene using Salmonella typhimurium TA100, TA97, TA104 and microsomes. Hakim Res J 2004; 24(1): 45-51. [Persian]
11. Bathini M, Goto S, Tian H, Ando F, Fukuhara M, Watanabe I. Mutagenicity of 1,3-Butadiene, 1,4- Pentadiene -3- ol, Isoprene, 2,4- Hexadiene, cis and trans- piprylene, Envi- ron. Health Perspect 2002; 3: 73-78.
12. Dorothy M, Ames BN. Revised meth-ods for the salmonella mutagenicity test. Mu-tat Res 1983; 113:173- 216.
13. Mortelmans K, Zeiger E. The Ames salmonella/microsome mutagenicity assay Mutat Res 2000; 455: 29-60.
14. Mccann J, Choi E, Yamasaki E, Ames BN. Detection of carcinogens in the Salmo-nella/ microsometest. Assay of 300 chemicals. Proc NatL Acad Sci USA 1975; 72: 5135-39.
15. Animal Care and Ethics committee, Islamic Azad University, Boroujerd Branch, Iran, 2012. Available from: http://www.iaub.ac.ir/English/ANIMALCAREANDETHICS.pdf
16. Berg K. Braun C. Krug I. Schrenk D. Evaluation of the cytotoxic and mutagenic potential of three ginkgolic acids. Toxicology 2015; 327, 47–52.
17. Berg K, Bischoff R, Stegmüller S, Cartus A, Schrenk D. Comparative investigation of the mutagenicity of propenylic and allylic asarone isomers in the Ames fluctuation assay. Mutagenesis 2016; 31:443–51.
18. Ames BN. Method for detecting carcinogens and mutagens with the Salmonella/ mammalin microsome mutagenicity test. Mutat Res 1976; 31; 347-49.http://tuberose.com/Teflon.html(2006).
20. Wakabayashi K, Watanabe T, Ohe T. Mutagens in surface water: a review. Muta-sion Res 2004; 567(2-3): 109-49.
21. Mehrabian S, Majd A, Kheiri A, Joniubi PA. Study of the antimutagenic effects of different extracts of Aloe vera leaf gel and latex using Ames test. Arak Med Uni J 2012; 15(2):100-6.
22. Ferraz MC, de Oliveira JL, de Oliveira Junior JR, Cogo JC, Dos Santos MG, Franco LM, et al. The triterpenoid betulin protects against the neuromuscular effects of bothrops jararacussu snake venom in vivo. Evid Based Complement Alternat Med 2015; 2015: 939523.
23. Krol SK, Kielbus M, Rivero-Muller A, Stepulak A. Comprehensive review on betulin as a potent anticancer agent. Biomed Res Int 2015; 2015: 584189.
24. Yoshida EH, Tribuiani N, Sabadim G, Neto Moreno DA, Varanda EA, and Franco YO. Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test. Adv Pharm Bull 2016; 6(3): 443–47.

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