Volume 26, Issue 12 (Mar 2018)
JSSU 2018, 26(12): 1027-1037 |
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Haghiralsadat B F, Amoabediny G, Naderinezhad S, Morteza-Ratki F, Zandieh Doulabi B. Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma. JSSU 2018; 26 (12) :1027-1037
URL: http://jssu.ssu.ac.ir/article-1-4067-en.html
URL: http://jssu.ssu.ac.ir/article-1-4067-en.html
Bibi Fatemeh Haghiralsadat 
, Ghasem Amoabediny * , Samira Naderinezhad , Farzane Morteza-Ratki , Behrouz Zandieh Doulabi
, Ghasem Amoabediny * , Samira Naderinezhad , Farzane Morteza-Ratki , Behrouz Zandieh Doulabi
Abstract: (3453 Views)
Introdution: Cationic liposomes have been presented for gene delivery as an alternative vector instead of viral vectors. A major challenge associated with siRNA delivery is the instability of liposomes, which is still a serious problem. The aim of this study was to provide an appropriate formulation to overcome this instability.
Methods: In the present study (Scientific-Fundamental, Experimental-Laboratory Study), liposomal formulation containing soy phosphatidylcholine, cationic DOTAP, cholesterol and polyethylene glycole was synthesized by thin-film hydration method and the siRNA were loaded on liposomes through incubation. In the following; the optimization of siRNA loading was on the agenda. Then the parameters related to size, zeta potential, polydispersity index and lon-term stability of siRNA-liposomes complex were reported. The Data were analyzed by GraphPad Prism version 7 Software. All data were repeated three times and reported as mean±standard deviation.
Results: In this study we were able to produce siRNA lipoplex with high loading efficiency of siRNA. The produced nanoparticles did not agglomerate and were stable at 4 oC for 3 months. This nanosystem could successfully deliver siRNA to normal bone cells. Studies have shown that the blank system (no gene) had no toxicity.
Conclusion: The prepared PEGylated liposomes have a great potential for delivery of siRNA to bone cells
Methods: In the present study (Scientific-Fundamental, Experimental-Laboratory Study), liposomal formulation containing soy phosphatidylcholine, cationic DOTAP, cholesterol and polyethylene glycole was synthesized by thin-film hydration method and the siRNA were loaded on liposomes through incubation. In the following; the optimization of siRNA loading was on the agenda. Then the parameters related to size, zeta potential, polydispersity index and lon-term stability of siRNA-liposomes complex were reported. The Data were analyzed by GraphPad Prism version 7 Software. All data were repeated three times and reported as mean±standard deviation.
Results: In this study we were able to produce siRNA lipoplex with high loading efficiency of siRNA. The produced nanoparticles did not agglomerate and were stable at 4 oC for 3 months. This nanosystem could successfully deliver siRNA to normal bone cells. Studies have shown that the blank system (no gene) had no toxicity.
Conclusion: The prepared PEGylated liposomes have a great potential for delivery of siRNA to bone cells
Keywords: SiRNA lipoplex, Physico-chemical characterization, Intracellular uptake, Cellular viability.
Type of Study: Original article |
Subject:
Pharmacology
Received: 2017/01/15 | Accepted: 2017/07/9 | Published: 2019/04/16
Received: 2017/01/15 | Accepted: 2017/07/9 | Published: 2019/04/16
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