Volume 26, Issue 5 (Agu 2018)                   JSSU 2018, 26(5): 399-409 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pourhavashemi S, Bamzadeh Z, Rouhi L, Zia-Jahromi N. Survey the effect of produced metabolites by native Pseudomonas sp. UW4 on p53 gene expression in SK-BR-3 breast cancer cell line . JSSU. 2018; 26 (5) :399-409
URL: http://jssu.ssu.ac.ir/article-1-3949-en.html
Abstract:   (330 Views)
Introdution: Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native Pseudomonas sp. UW4 metabolite to find new compound.
Methods: This experimental study was performed in Shahrekord Islamic Azad University from April 2015 to August 2015. First SK-BR-3 human breast cancer cells with different concentrations (5, 10 and 20 mg/ml) were treated with produced metabolite by native Pseudomonas sp. UW4 for 24 and 48 hours. Cell viability was assessed by MTS assay. Then, p53 expression was detected by RT-Real Time PCR. For statistical analysis, SPSS16 software and one way ANOVA test were used.
Results: Treatment with produced metabolite by the native Pseudomonas sp. UW4 showed the decreases the viability of cells in a time and dose dependent manner, the most effective concentration of this substance was 20 mg/ml and 48 h after treatment. Also, an increase in p53 gene expression, significantly in 10 and  20 mg/ml after 24 h and 5, 10 and 20 mg/ml after 48h was observed (P<0.05).
Conclusion: Produced metabolite by native Pseudomonas sp. UW4 could be used for treatment of SK-BR-3 breast cancer.
Full-Text [PDF 1103 kb]   (147 Downloads)    
Type of Study: Original article | Subject: Biology
Received: 2016/11/2 | Accepted: 2017/02/18 | Published: 2018/10/22

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2018 All Rights Reserved | SSU_Journals

Designed & Developed by : Yektaweb