The Survey of Contamination of Platelet Product with  Aerobic Bacteria in Isfahan Blood Transfusion Center

Baghban , F; Baghi Baghban , F; Bamzadeh , Z; Akbari , N; Khosravi Bakhtiari , M

Volume 24, Issue 6 (sep 2016) JSSU 2016, 24(6): 441-448 | Back to browse issues page

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Baghban F, Baghi Baghban F, Bamzadeh Z, Akbari N, Khosravi Bakhtiari M. The Survey of Contamination of Platelet Product with Aerobic Bacteria in Isfahan Blood Transfusion Center. JSSU 2016; 24 (6) :441-448
URL: http://jssu.ssu.ac.ir/article-1-3696-en.html

The Survey of Contamination of Platelet Product with Aerobic Bacteria in Isfahan Blood Transfusion Center

F Baghban ^*

, F Baghi Baghban

, Z Bamzadeh

, N Akbari

, M Khosravi Bakhtiari

Abstract: (5698 Views)

Introduction: Although nowadays the risk of transmission of bacterial pathogens through blood transfusion has been decreased, but there is the possibility of transmission of these factors by injection of these kind of products. The purpose of this survey was determination of contamination of platelet products with aerobic bacteria in Isfahan Blood Transfusion Center.

Methods: In the spring and summer of 2014, 2000 platelet product samples were examined randomly in 5 months for aerobic bacterial contamination. First, samples were cultured in fluid thioglycollate medium. The bacteria that were grown in this medium were identified by Gram staining and biochemical tests. Then, DNA was extracted from isolated bacteria and PCR was done for 16S rRNA gene. After that the PCR products were sequenced and the bacteria were recognized at the level of species.

Results: At this research, 4 contaminated samples were identified. Isolated bacteria were including: Klebsiella pneumoniae 1 case, Staphylococcus aureus 1 case, Staphylococcus epidermidis 1 case and Staphylococcus haemolyticus 1 case. After sequencing of 16S rRNA gene, the homology was observed 97%, 83%, 99%, and 90% at theses bacteria, respectively.

Discussion: According to the results of this research, platelet products may be contaminated with aerobic bacteria. Therefore, providing appropriate conditions in transfusion centers and other therapeutic centers for doing screening tests on platelet products to identifying bacterial contaminations before using of these products seems to be necessary.

Keywords: Platelet Products, Bacterial Contamination, PCR

Full-Text [PDF 479 kb] (2398 Downloads)

Type of Study: Original article | Subject: Microbiology
Received: 2016/04/20 | Accepted: 2016/08/6 | Published: 2016/10/9

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